Variants in DENND1A and LHCGR are associated with endometrioid adenocarcinoma

Objective

The aim of this study was to explore the polycystic ovary syndrome (PCOS) related single nucleotide polymorphisms (SNPs) rs13405728 (in gene LHCGR), rs13429458 (in gene THADA) and rs2479106 (in gene DENND1A) in women with endometrial carcinoma.

Methods

We conducted a case-control study comprising 96 Han Chinese women with endometrial carcinoma, and 192 healthy controls. SNPs rs13405728, rs13429458 and rs2479106 were genotyped by polymerase chain reaction (PCR) and direct sequencing. The effects of body mass index (BMI) and age were evaluated using an unconditional logistic regression model adjusted for potential confounders.

Results

The allele frequencies of SNPs rs2479106 and rs13405728 were significantly different (P < 0.05) between endometrial carcinoma group and control group, and the difference was especially significant in the subgroup of endometrioid adenocarcinoma. Genotyping analysis showed that allele G in rs2479106 and allele A in rs13405728 could confer risk to endometrioid adenocarcinoma.

Conclusions

Our results suggest that SNPs rs2479106 in gene DENND1A and rs13405728 in gene LHCGR are associated with endometrioid adenocarcinoma.     

多囊卵巢综合征患者白细胞计数的变化特点

目的 探讨多囊卵巢综合征(PCOS)患者外周血白细胞(WBC)计数与体质量指数(BMI)、总睾酮(T)、雌二醇(E2)和促黄体生成素(LH)的关系。方法 对1016例PCOS患者(PCOS组)和1016例正常女性(对照组)的人体测量研究结果和基础内分泌指标进行回顾性分析,比较两组外周血WBC总数及其分类中性粒细胞(N)和淋巴细胞(L)计数,并分析PCOS患者外周血WBC总数及其分类N、L计数与BMI、T、E2、LH的相关性。结果 PCOS患者的外周血WBC总数和L计数明显高于正常女性(P<0.05),WBC总数和L计数的升高与PCOS患者的BMI增加有关,与高水平E2和高水平LH不相关(P＞0.05),WBC总数的升高与T呈正相关性(r=0.073,P<0.05),L计数的升高与T不相关(P＞0.05)。结论 PCOS患者的WBC总数和淋巴细胞计数明显高于正常女性。     

大鼠视网膜干细胞的增殖和多向分化**

背景：国内对视网膜干细胞的体外分离培养及鉴定仍处于初步探索阶段。 目的：体外分离、培养及鉴定新生大鼠视网膜干细胞,探讨其多向分化潜能。 方法：用神经干细胞无血清培养方法分离和培养新生24 h的SD大鼠睫状体细胞,第6代视网膜干细胞经胎牛血清诱导分化,应用免疫细胞化学方法检测视网膜干细胞的分化特性。 结果与结论：体外培养的细胞球具有连续克隆能力,Nestin抗原阳性,BrdU 标记结果显示悬浮细胞团主要由分裂增殖的细胞组成,并表达胚胎发育早期视网膜内原始细胞的特异性抗原Chx-10;诱导分化后的细胞表达星形胶质细胞特异性标志物GFAP、神经元特异性标志物NSE、感光细胞标志物Opsin、双极细胞特异性抗原PKC和节细胞特异性抗原β-tubulin,实验初步证实培养的视网膜干细胞具有神经干细胞特性,能自我更新和增殖分化成为感光细胞类型的细胞。 关键词：视网膜干细胞;细胞培养;无血清;细胞分化;大鼠 doi:10.3969/j.issn.1673-8225.2012.06.015     

Effects of cumulus cells on vitreous cryopreservation of human mature oocytes and clinical pregnancy outcomes

The purpose of this study was to explore the effects of cumulus cells on vitreous cryopreservation of human mature oocytes and clinical pregnancy outcomes. The study was divided into group A (cumulus cells were removed from the oocytes before freezing) containing 24 participants and 193 oocytes and group B (cumulus cells were retained with the oocytes before freezing) containing 26 participants and 240 oocytes. Based on no significant differences in age, duration of infertility, infertile causes, and number of retrieved oocytes between both groups when oocytes were retrieved from infertile women, we found that the survival rate of post thaw oocytes (88% vs 58%), cleavage rate (80% vs 56%), and high-quality embryo rate (75% vs 59%) were significantly higher in group B than in group A. Under the conditions that there were no significant differences between the 2 groups in the general status of the participants undergoing embryo transfer, the embryo implantation rate (37% vs 15%) and the clinical pregnancy rate (50% vs 17%) were significantly higher in group B than in group A, all with Ps < .05. We conclude that the retention of cumulus cells can improve the developmental competence of vitrified-thawed human mature oocytes and clinical pregnancy outcomes.     

Male chromosomal polymorphisms reduce cumulative live birth rate for IVF couples

Purpose

Chromosomal polymorphisms are associated with infertility, but their effects on assisted reproductive outcomes are still quite conflicting, especially after IVF treatment. This study evaluated the role of chromosomal polymorphisms of different genders in IVF pregnancy outcomes.

Methods

Four hundred and twenty-five infertile couples undergoing IVF treatment were divided into three groups: 214 couples with normal chromosomes (group A, control group), 86 couples with female polymorphisms (group B), and 125 couples with male polymorphisms (group C). The pregnancy outcomes after the first and cumulative transfer cycles were analyzed, and the main outcome measures were live birth rate (LBR) after the first transfer cycle and cumulative LBR after a complete IVF cycle.

Results

Comparison of pregnancy outcomes after the first transfer cycle within group A, group B, and group C demonstrated a similar LBR as well as other rates of implantation, clinical pregnancy, early miscarriage, and ongoing pregnancy (P > 0.05). However, the analysis of cumulative pregnancy outcomes indicated that compared with group A, group C had a significantly lower LBR per cycle (80.4 vs 68.00%), for a rate ratio of 1.182 (95% CI 1.030 to 1.356, P = 0.01) and a significantly higher cumulative early miscarriage rate (EMR) among clinical pregnancies (7.2 vs 14.7%), for a rate ratio of 0.489 (95% CI 0.248 to 0.963, P = 0.035).

Conclusion

Couples with chromosomal polymorphisms in only male partners have poor pregnancy outcomes after IVF treatment manifesting as high cumulative EMR and low LBR after a complete cycle.

     

The low fetal fraction at the first trimester is associated with adverse pregnancy outcomes in IVF singleton pregnancies with single embryo transfer from frozen cycles

Journal of Assisted Reproduction and Genetics - To study the associations between fetal fraction at the first trimester and subsequent adverse pregnancy outcomes&nbsp;(APOs) in IVF singleton...