酵母样真菌感染的菌群临床分布与药敏分析

目的了解临床酵母样真菌的感染类型、分布及其对常用抗真菌药物的敏感性,为临床诊断治疗提供正确的用药依据。方法采用常规方法分离培养真菌,用法国生物梅里埃公司生产的ATB Expression微生物自动鉴定/药敏分析仪进行真菌鉴定和药敏分析。结果396株酵母样真菌中,白色假丝酵母菌检出率最高(72.3%),其次为热带假丝酵母菌(15.91%),光滑假丝酵母菌(6.82%)。酵母样真菌对5-氟胞嘧啶、两性菌素B、氟康唑、依曲康唑的敏感性分别为98.02%,98.43%,77.38%,77.76%。结论临床酵母样真菌感染以白色假丝酵母菌为主,对5-氟胞嘧啶、两性菌素B敏感性较高,对氟康唑、依曲康唑有不同程度的耐药,因此,药敏监测是非常必要的,临床医生应根据药敏结果合理使用抗真菌药物。     

Reframing climate change as a public health issue: an exploratory study of public reactions

Background  

Climate change is taking a toll on human health, and some leaders in the public health community have urged their colleagues to give voice to its health implications. Previous research has shown that Americans are only dimly aware of the health implications of climate change, yet the literature on issue framing suggests that providing a novel frame - such as human health - may be potentially useful in enhancing public engagement. We conducted an exploratory study in the United States of people's reactions to a public health-framed short essay on climate change.     

Changes of circulating TNF and its effects on Pseudomonas aeruginosa septic shock in rabbits

Itiswellknownthatcertaincytokinesplayapivotalroleinthedevelopmentoftraumaandshock.Tumornecrosisfactor(TNF)isapolypep-tiedcytokinesynthesizedinmonocytesandmacrophagesinresponsetolipopolysaccharide(LPS)stimulation-TherelationshipbetweenTNFandendotoxic-septicshockbecomesafocusoftheresearchofshockmediators[1'2].Alargeamountofexperimentalandclinicaldata[3-9JsuggestthatTNFplaysanimportantroleinthepathogenesisofendotoxic-septicshockbuttheprecisemechanismandtherelationshipofTNFtoshockseverityrem…     

A comparative study of using comet assay and gammaH2AX foci formation in the detection of N-methyl-N'-nitro-N-nitrosoguanidine-induced DNA damage.

Comet assay is a useful technique in the detection of DNA damages, particularly DNA strand breaks; and it has been utilized to show that a potent carcinogen N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), can induce such damages. Recently, gammaH2AX foci formation has been suggested as another sensitive way to detect DNA double strand breaks (DSBs). However, there is no systematic comparison being conducted to evaluate the consistency of these two methods. Using MNNG as a model chemical, the sensitivity of neutral comet assay and gammaH2AX foci formation in detecting MNNG-induced damage was studied. It was found that at concentrations of 0.1 and 1 microg/ml, both methods can detect MNNG-induced damage in human amnion FL cells. However, at 0.1 microg/ml, comet assay revealed more percentage of cells with DNA damage than gammaH2AX fluorescence revealed. On the other hand, while gammaH2AX foci were readily formed at very early times by 10 microg/ml MNNG treatment, neutral comet assay did not detect any significant DNA damage at the same time points. In addition, 10 microg/ml MNNG induced a distinct whole nuclei staining pattern of gammaH2AX, a type of DNA damage which was not detected by neutral comet assay but could be detected by alkaline comet assay. Therefore, gammaH2AX may be used as a sensitive indicator for DNA damage.     

Effects of hemorrhagic shock on endotoxemia and its molecular mechanism

AlthoughtherearegrowingevidencesindicatlugthatonlyweakendotoxemlaexistsintheearlystageafterhemorrhagicshockinhumanbeingsoranirnalsLI-3,antagonistsagainstendotoxinssuchasbacterlcldal/permeablllty--lucreproteinf'j,polymyxinBL4'andlactuloseL',exertsignificantprotectiveeffectsonthevictimsinshock.Thisseemsthatbacterlalendotoxinsplayanimportantroleinthedevelopmentofhemorrhagicshock.However,itremainsunclarifiedwhethertheroleofendotoxinsinshockisrelatedtotheincreaseofanIndividual'ssensitivitytoendot…     

CT-guided stereotactic injection of radionuclide in treatment of brain tumors.

140 patients with brain tumor were treated by CT-guided stereotactic injections of radionuclides, such as Aurum-198 (198 Au), Phosphorus-32 (32 P) and Yttrium-90 (90Y). Of these patients aged from 3 to 67 years (average 37), 64 were male and 76 female. Astrocytoma was found in 75 patients, craniopharyngioma in 46, metastatic carcinoma in 7, meningioma in 5, germinoma in 4 and pituitary adenoma in 3. The tumors were located in the deep part or functionally critical area of the brain. After 267 times of injection of colloidal isotopes, no major adverse effects or complications occurred. Follow-up for 6 to 48 months showed improvement in symptoms in 104 (74.3%) patients and CT scanning showed the diminished tumors.

     

氧氟沙星薄膜包衣片的制备及其相对生物利用度测定

本文采用国产原料试制氧氟沙星薄膜包衣片，并与日本第一制药株式会社生产的片剂进行了体外溶 出度、休内相对生物利用度比较。休内血清药物浓度采用HPLC法测定。自制片剂与进口片剂生物等效。     

Gd(一)台湾-客家型部分基因克隆化分离和鉴定

从两例萄糖一6一磷酸脱氢酶变异型——台湾客家型[Gd(-)Taiwan-Hakka]患者基因组DNA中,回收经EcORⅠ完全酶解的3～5 kb片段,与载体λgt10插入重组,用SMR10单菌种系统制备包装提取物,对重组的λgt10进行体外包装,构建成两个基因组基因文库。用~(32)P中标记的G6 PDcDNA成功地从文库中初选出12个阳性克隆,挑选其中4个阳性克隆再次杂交予以证实,提取其中2个克隆DNA,经限制酸酶切电泳及斑点杂交鉴定,分离到该变异型基因的插入片段,并将其克隆到M13 mP19噬菌体,完成Gd(-)Taiwan-Hakka型部价基因克隆化分离与鉴定,为DNA序列分析寻找突变部位打下基础。     

医疗卫生单位的后勤社会化管理与改革

相对于我国企业改革的发展,医疗卫生单位后勤管理与改革不仅起步较晚,而且力度不大,步伐不快。以往的医疗卫生单位后勤往往面面俱到,造成了严重的经济负担和社会资源的浪费。因此,医疗卫生单位后勤改革势在必行。     

塑料镜片材料的发展动向

概述光学塑料的基本材料，讨论塑料镜片材料的主要品种及其应用，并着重分析影响塑料镜片材料性能的主要因素及其发展趋势。