冷适应大鼠肝脏线粒体的呼吸功能及蛋白质合成

从正常和冷冻大鼠肝脏中分离出线粒体。用氧电极法测定鼠肝线粒体呼吸功能,包括ADP:O比值(P/O值),呼吸控制率(RCR),以[~3H]-Leu参入为指标,观察线粒体内蛋白质合成;以SDS-聚丙烯酰胺凝胶电泳方法观察线粒体内膜蛋白质的变化。结果表明:冷适应2、4周时P/O值和RCR降低,6周时两指标恢复至正常水平。冷适应1,2,4周时线粒体蛋白质合成明显降低。冷适应2,3周时线粒体内膜蛋白质电泳图谱呈现明显变化,冷适应组线粒体内膜有新的蛋白质条带出现。     

低温对鼠肝亚线粒体膜流动性的影响

环境温度可以对生物体的能量代谢产生明显影响,植物膜流动性与温度有关,而且发现低温可以对水稻和玉米线粒体膜的流动性产生明显影响。本研究以荧光偏振度为指标,观察了暴露于寒冷环境(冷暴)数周后大鼠肝脏亚线粒体膜流动性的变化,旨在探讨环境温度影响生物膜结构与功能的机理。1 材料和方法 实验选用SD.纯系大鼠68只,雄性,120～150 g,80～100d龄。配对分为正常组和低温组。 大鼠断头放血取肝脏,用线粒体分离液(250mmol/L蔗糖,10 mmol/L Tris,1 mmol/L EDTA     

甲胎蛋白对肝癌细胞生长的影响 Ⅰ.人AFP对小鼠H_(22)腹水型肝癌细胞RNA合成的促进作用

用亲和层析法从3-5月龄引产胎儿肌肉组织中提纯AFP。体外用~3H-UR参入的方法,从转录水平观察AFP对小鼠H_(22)腹水型肝癌细胞生长的影响。结果表明,人AFP能显著地直接促进该肿瘤细胞RNA的合成;人血白蛋白和去除AFP后胎儿肌肉匀浆液等对照均无显著作用;AFP抗血清能显著地清除AFP的这种促进作用。用小鼠S_(180)乳腺肉瘤细胞作为对照表明,AFP促肿瘤生长无严格的组织特异性。     

A study on liver mitochondria respiration and protein synthesis in cold adapted rats

Mitochondria were isolated from normal and cold adapted rat livers.The respiratory func-tion of mitochondria in rat livers,including ADP:O ratio(P/O)and the respiratory control ratio(RCR),was determined by oxygen electrode method,The protein synthesis in mitochondria wasstudied by observing the incorporation of[~3H]-Leucine into mitochondria.Polyacrylamide gelelectrophoresis was carried out to detect the changes of the inner membrane proteins.It was shownthat the P/O and RCR decreased in cold adapted rats in the 2nd and 4th weeks and returned tothe control level in the 6th week,the protein synthesis of mitochondria decreased significantly incold adapted rats in 1,2 and 4 weeks;the electrophoretic pattern of the inner membrane proteinsin mitochondria from cold adapted rat livers revealed some new bands.