| 冷适应大鼠肝脏线粒体的呼吸功能及蛋白质合成 |
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| 引用本文： | 何开跃,杨泽田.冷适应大鼠肝脏线粒体的呼吸功能及蛋白质合成[J].第四军医大学学报,1988(6). |
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| 作者姓名： | 何开跃 杨泽田 |
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| 作者单位： | 第四军医大学 403—4室
(何开跃)，第四军医大学 403—4室(杨泽田) |
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| 摘 要： | 从正常和冷冻大鼠肝脏中分离出线粒体。用氧电极法测定鼠肝线粒体呼吸功能,包括ADP:O比值(P/O值),呼吸控制率(RCR),以~3H]-Leu参入为指标,观察线粒体内蛋白质合成;以SDS-聚丙烯酰胺凝胶电泳方法观察线粒体内膜蛋白质的变化。结果表明:冷适应2、4周时P/O值和RCR降低,6周时两指标恢复至正常水平。冷适应1,2,4周时线粒体蛋白质合成明显降低。冷适应2,3周时线粒体内膜蛋白质电泳图谱呈现明显变化,冷适应组线粒体内膜有新的蛋白质条带出现。
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| 关 键 词： | 冷 适应 生理 线粒体 肝 氧化磷酸化 膜蛋白 大鼠 |
A Study on Liver Mitochondria Respiration and Its Protein Synthesis in Cold Adapted Rats |
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| He Kaiyao and Yang Zetian Labcratcry -.A Study on Liver Mitochondria Respiration and Its Protein Synthesis in Cold Adapted Rats[J].Journal of the Fourth Military Medical University,1988(6). |
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| Authors: | He Kaiyao and Yang Zetian Labcratcry - |
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| Institution: | He Kaiyao and Yang Zetian Labcratcry 403-4 |
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| Abstract: | t Mitochondria were isolated from the normal and cold adapted rat livers. The respiratory function of mitochondria in rat livers, including ADP: O ratios (P/O) and the respiratory control ratio (RCR), was determined with oxygen electrode. The protein synthesis in mitochondria was studied by observing the incorporation of 3H-Leucine into mitochondria. Polyacrylamide gel electrophoresis was carried out for detecting the change of the inner membrane protein. It was shown that the P/O and RCR decreased in cold adapted rats in the 2nd and 4th weeks and recovered to the control level in the 6th week;the protein synthesis of mitochondria decreased significantly in cold adapted rats in 1,2 and 4 weeks; the electrophoresis pattern of the inner membrane proteins in mitochondria from cold adapted rat livers revealed some new bands. |
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| Keywords: | cold adaptation mitochondria live oxidative phosphorylation respiratory control ratio incorporation of [3H]-Leu membrane proteins |
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