排序方式: 共有6条查询结果,搜索用时 31 毫秒
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人红细胞单链抗体基因的克隆及其序列分析 总被引:4,自引:1,他引:3
目的:获得抗人红细胞的单链抗体基因。方法:以分泌抗人红细胞单抗(HRC)的杂交瘤细胞总RNA为模板,分别扩增出轻链可变区(VL)和重链可变区(VH)基因,用连接肽将其连接成具VL-linker-VH结构的单链抗体基因。结果:DNA序列分析证实,获得的单链抗体基因的轻,重链分别属于鼠Igkappa轻链第Ⅲ亚组和Ig重链第Ⅱc亚组,结论:构建了抗人红细胞单链抗体的载体,提供了与其它基因连接,为建立病人 相似文献
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Jiang Yutu蒋豫图 Li Zhongduo李钟铎and Song Guangchang 宋光昌Institute o于Microbiolog''y EpidemioZogy Academy of Mil itar''y Medical Sciences Beijing 《中华医学杂志(英文版)》1981,94(4):221-228
Sera from 193 cases of epidemic hemorrhagic
fever renal syndrome (EHFRS) and 173 normal
persons and patients with other diseases in
endemic and nonendemic areas were examined
for immunofluorescent (IF) antibodies to Korean
hemorrhagic fever (KHF) antigen by indirect
immunofluorescent antibody (lFA) test. 76 0f
78 (97.4470) paired sera from EHFRS patients
were KHF' antibody positive in l or both samples.
Of 109 acute stage sera samples 81 (74.31To) were
KHF IgM antibody positive whereas of 162 con-
valescent sera samples 156 (96.2970) were KHF
IgG antibody positive, indicating that the EHFRS
agent is immunologically related, if not identical
with KHF.
The earliest date of IF IgM antibody ap-
pearance was l day after onset of illness a.nd
IF IgG antibodies could be found in the patient
convalescent serum 16 years after the acute at-
tack. There was no immunologic relationship
between the EHFRS agent and Japanese B en-
cephalitis (JBE) or influenza A3 viruses. 相似文献
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利用细胞融合技术先后建立了抗人γ链和抗人μ链杂交瘤细胞株,制备出腹水单克隆抗体。用荧光素(FITC)和辣根过氧化物酶(HRP)分别标记作为第二抗体用于免疫荧光染色、酶免疫细胞化学染色、酶联免疫吸附试验(ELISA)和Western印迹等方法,对综合征出血热、肝炎、艾滋病等病人血清进行测定,取得良好效果。试验证明,这两个单克隆抗体特异性好、亲和力强、敏感性高,适用于多种实验诊断方法,是微生物免疫诊断 相似文献
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人源汉坦病毒H8205株S基因NP编码区的序列测定及其NP强抗 … 总被引:1,自引:1,他引:0
目的:确定人源汉坦病毒H8205株在汉坦病毒属中的分类地位及找出核衣壳蛋白(nucleocapsid,NP)强抗原性的分子生物学基础。方法:采用半套式RT-PCR扩增H8205S基因核衣壳蛋白(NP)编码区,克隆于载体pBV220中并进行序列测定。测序结果用PCGENE分析。结果:H8205株S基因NP编码区为1290bp(含两端引物序列)与76-118株核苷酸的同源性为83.0%,氨基到的同源性 相似文献
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