中药木鳖子中脂肪酸的气相色谱-质谱联用分析

目的：测定中药木鳖子中的脂肪酸成分。方法：以石油醚为溶剂，采用索氏提取法提取其脂肪酸。经甲酯化处理后，用GC／MS联用仪对其脂肪酸组成进行了分析和鉴定。结果：共分离出14种脂肪酸，占脂肪酸总量的89．23％。结论：木鳖子脂肪酸中不饱和脂肪酸占41．91％，且大部分是具生物活性物质。     

紫外线蓝光照射加树脂吸附净化人工肝分离血浆

非生物人工肝常用的血浆灌流是通过树脂吸附或离子交换吸附除去血浆中的胆红素,其胆红素清除率仅40％左右。这可能是因为胆红素与白蛋白结合比较紧密,无法被有效吸附清除。用于治疗新生儿黄疸的蓝光照射可使不溶于水的未结合胆红素转变成水溶性胆绿素,促使胆红素排泄,     

碱化血浆微囊炭吸附法净化人工肝置换的血浆

目的探讨碱性化血浆对树脂吸附血浆胆红素的影响,以便净化回收人工肝置换的血浆。方法在体外将人工肝血浆置换法置换下来的血浆pH值调为10,再滤过微囊炭吸附柱吸附胆红素,再将滤过后的血浆pH调为7.4,即为净化血浆。结果碱化血浆经微囊炭吸附,可清除86.6%的IBil和82.5%的TBil,可回收95%白蛋白;未碱化血浆经微囊炭吸附,可清除76.5%的IBil和73.4%的TBil,可回收90%白蛋白。碱化血浆和未碱化血浆吸附后胆红素剩余量或清除率有明显降低或增加（P〈0.01或P〈0.05）。结论本文在体外建立的血浆（或碱化血浆）微囊炭吸附法都可有效清除人工肝置换的血浆中胆红素,以碱化血浆法为更好。     

紫外线蓝光照射和树脂吸附清除血浆胆红素

Objective To study the clearance of bilirubins from the plasma by ultraviolet-blue ray irradiation and resin adsorption. Methods The plasma from artifitial liver treatment were irradiated by the ultraviolet-blue ray and then filtrated the strongly basic anion exchange resin(D201) or adsorbent resin(D4006) to eliminate bilirubins. Results ① The mean level of total bilirubins(TBil), direct bilirubin( DBil) and indirect bilimbin( IBil) decreased significantly after the irradiation( P <0.01) ,and there was no significant decrease about albumin after the irradiation. ②For the group of irradiation then D201 adsorption, the clearance rates of TBil, DBil and IBil were 85.3% , 85.8% and 85.1% respectively; for the group of D201 adsorption without the irradiation, the rates were 58.3% ,50.8% and 61.7% respectively, and there was statistical difference between the two groups ( P < 0.05, P < 0.01). There were no difference between two groups in the recovery rates of total protein and albumin( P > 0.05). ③For the group of irradiation then D4001 adsorption, the clearance rates of TBil, DBil and IBil were 71.0%, 74.5% and 68.6% respectively; for the group of D4006 adsorption without the irradiation, the rates were 19.8%, 17.6% and 21.2% respectively, and there was statistical difference between the two groups ( P < 0.01). There were no difference between two groups in the recovery rates of total protein and albumin( P > 0.05). ④For the groups of irradiation followed by the resin adsorption, the clearance rate of IBil by D4006 (68.6%) was lower than that by D201 (85.1%)(P<0.05), but the recovery of albumin by D4006 was more than that by D201 (P < 0.05). Conclusions The ultraviolet-blue ray irradiation on plasma could directly decrease bilirubin concentrations and the irradiation followed by the resin adsorptions could decrease bilirubin concentrations further with a little influence to the recovery of albumin.     

碱化蛋白溶液和碱化血浆树脂吸附清除胆红素的效果比较

我们曾将用盐析法回收的血浆蛋白溶液碱化,可明显增加活性炭吸附清除胆红素的量~([1]).在此基础上,我们对碱化蛋白溶液和碱化血浆树脂吸附清除胆红素的效果进行了比较,现报道如下.     

病毒性肝炎患者血清TNF-α,IL-6与肝纤维化指标关系的探讨

目的　探讨病毒性肝炎患者血清TNF -α,IL - 6及Ⅲ型胶原N端肽 (PⅢNP)、Ⅳ型胶原 (Col -Ⅳ )和透明质酸 (HA)与肝纤维化的关系。方法　采用ELISA检测患者血清TNF -α、IL - 6、PⅢNP、Col-Ⅳ的含量 ;RIA检测HA的含量。结果　病毒性肝炎患者血清中TNF -α和IL - 6含量密切相关 (r=0 .6 5 8,P<0 .0 1) ,各肝炎组明显高于正常对照组 (P <0 .0 1)。TNF -α与IL - 6 ,PⅢNP ,Col -Ⅳ和HA呈显著正相关 ;IL - 6与TNF -α,PⅢNP ,Col-IV和HA呈显著正相关。除慢肝轻度组外 ,各肝炎组PⅢNP、Col -Ⅳ、HA含量均显著高于正常组 (P <0 .0 1) ,且慢肝重度 >中度 >轻度 (P <0 .0 1)。结论　病毒性肝炎患者血清TNF -α和IL - 6的含量能反映肝脏的炎症及纤维化程度 ,可作为判断病情及预后的参考指标。证实PⅢNP ,Col-Ⅳ和HA均为反映肝脏炎症及纤维化的良好指标。     

紫外线蓝光照射和树脂吸附清除血浆胆红素

Objective To study the clearance of bilirubins from the plasma by ultraviolet-blue ray irradiation and resin adsorption. Methods The plasma from artifitial liver treatment were irradiated by the ultraviolet-blue ray and then filtrated the strongly basic anion exchange resin(D201) or adsorbent resin(D4006) to eliminate bilirubins. Results ① The mean level of total bilirubins(TBil), direct bilirubin( DBil) and indirect bilimbin( IBil) decreased significantly after the irradiation( P <0.01) ,and there was no significant decrease about albumin after the irradiation. ②For the group of irradiation then D201 adsorption, the clearance rates of TBil, DBil and IBil were 85.3% , 85.8% and 85.1% respectively; for the group of D201 adsorption without the irradiation, the rates were 58.3% ,50.8% and 61.7% respectively, and there was statistical difference between the two groups ( P < 0.05, P < 0.01). There were no difference between two groups in the recovery rates of total protein and albumin( P > 0.05). ③For the group of irradiation then D4001 adsorption, the clearance rates of TBil, DBil and IBil were 71.0%, 74.5% and 68.6% respectively; for the group of D4006 adsorption without the irradiation, the rates were 19.8%, 17.6% and 21.2% respectively, and there was statistical difference between the two groups ( P < 0.01). There were no difference between two groups in the recovery rates of total protein and albumin( P > 0.05). ④For the groups of irradiation followed by the resin adsorption, the clearance rate of IBil by D4006 (68.6%) was lower than that by D201 (85.1%)(P<0.05), but the recovery of albumin by D4006 was more than that by D201 (P < 0.05). Conclusions The ultraviolet-blue ray irradiation on plasma could directly decrease bilirubin concentrations and the irradiation followed by the resin adsorptions could decrease bilirubin concentrations further with a little influence to the recovery of albumin.     

鲎试剂法检测香丹注射液中的细菌内毒素

目的 建立鲎试剂检测香丹注射液中的细菌内毒素的方法 .方法 用两个厂家的鲎试剂对四批样品进行干扰试验.结果 对样品进行1:9倍稀释,用0.25 Eu/mL鲎试剂进行试验可消除干扰.结论 可以应用鲎试剂法检测香丹注射液中的细菌内毒素.     

紫外线蓝光照射和树脂吸附清除血浆胆红素

目的 探讨紫外线蓝光混合光照射血浆联合树脂吸附对血浆蛋白及胆红素的影响,初步建立紫外线蓝光和(或)树脂吸附净化血浆方法.方法 将人工肝分离出的原血浆先用紫外线蓝光照射,再用D201型大孔强碱性阴离子交换树脂或D4006型非极性大孔吸附树脂吸附,根据采用的方法不同分为吸附组、照射组和照射吸附组,并与原血浆进行对照.观察在不同处理阶段(照射组、照射吸附组)及单纯吸附组蛋白和胆红素浓度变化.结果 ①照射组总胆红素(TBil)、直接胆红素(DBil)和间接胆红素(IBil)浓度明显低于原血浆组(P<0.01),血浆蛋白照射前后差异无统计学意义(P>0.05).②用D201树脂吸附时,照射吸附组胆红素(TBil、DBil和IBil)清除率分别为85.3%、85.8%和85.1%,吸附组分别为58.3%、50.8%和61.7%,两组的胆红素清除率和浓度差异有统计学意义(P<0.05,P<0.01);两组总蛋白、白蛋白差异无统计学意义(P>0.05).③用D4006树脂吸附时,照射吸附组胆红素(TBil、DBil和IBil)清除率分别为71.0%、74.5%和68.6%,吸附组分别为19.8%、17.6%和21.2%,两组胆红素清除率和浓度差异均有统计学意义(P<0.01);两组总蛋白、白蛋白差异无统计学意义(P>0.05).④在照射吸附组中,用D4006树脂时IBil清除率(68.6%)低于用D201树脂时IBil清除率(85.1%)(P<0.05),使用D4006树脂组血浆白蛋白高于使用D201树脂组(P<0.05).结论 紫外线蓝光照射血浆可直接降低胆红素浓度,明显提高阴离子交换树脂和吸附树脂胆红素清除率,对血浆蛋白回收影响较小,因此紫外线蓝光混合照射联合树脂吸附有望用于人工肝治疗.