论护士长的管理技巧对团队凝聚力的影响

阐述护士长的自身素质和管理技巧对团队凝聚力的影响及护士长应具备的管理技巧,认为提高护士长的自身素质和管理技巧是充分调动护理人员工作积极性从而提高护理质量的必要条件。     

体外不同比例再生肝细胞与结肠癌细胞的共培养

Objective To investigate the proliferation potential of colon cancer cells co-cultured with different proportions regenerating hepatocytes and role of the cell growth factors related to liver regeneration in vitro.Methods Active regenerating hepatocytes were obtained by collagenase perfusion in situ of rats models underwent 70% liver resection after 24 hours.Co-cultures with different proportions of hepatocytes to human colon cell line SW480 were performed respectively.The proportion of hepatocytes to human colon cell line SW480 Was 10:1 in group A,1:1 in group B.There was only human colon cell line SW480 in group C(control group).Proliferation capacity was assessed with the percentage by 3H-thy incorporation.Concentration of epidermal growth factor(EGF),insulin-like growth factor-1(IGF-1)and hepatocyte growth factor(HGF)was analyzed by ELISA.Results There was no significant difference among the 3 groups in 3H-thy incorporation percentage,and concentration of EGF,IGF-land HGF after 24 hours' culture(P>0.05).3H-thy incorporation percentage(15.9±1.4,13.2±1.5),and concerntration of EGF [(722.9±55.4)ng/L,(498.2+41.5)ng/L]and IGF-1[(755.2±35.7)ng/L,(538.1±37.5)ng/L]in Group A and B were higher than that in group C after 72 hours(P<0.05).Especially the indexes above in group A were higher than that in group B(P<0.05).there was no significant difference in concerntration of HGF among the 3 groups after 72 hours(P>O.05).Conclusions These results imply that the regenerating hepatocytes contribute to the hyperproliferative state of co-culturing colon cancer cells.The mechanism maybe have relationship with EGF and IGF-1 high expression in colon cancer cells caused by growth signals coming from hepatocytes.     

体外不同比例再生肝细胞与结肠癌细胞的共培养

Objective To investigate the proliferation potential of colon cancer cells co-cultured with different proportions regenerating hepatocytes and role of the cell growth factors related to liver regeneration in vitro.Methods Active regenerating hepatocytes were obtained by collagenase perfusion in situ of rats models underwent 70% liver resection after 24 hours.Co-cultures with different proportions of hepatocytes to human colon cell line SW480 were performed respectively.The proportion of hepatocytes to human colon cell line SW480 Was 10:1 in group A,1:1 in group B.There was only human colon cell line SW480 in group C(control group).Proliferation capacity was assessed with the percentage by 3H-thy incorporation.Concentration of epidermal growth factor(EGF),insulin-like growth factor-1(IGF-1)and hepatocyte growth factor(HGF)was analyzed by ELISA.Results There was no significant difference among the 3 groups in 3H-thy incorporation percentage,and concentration of EGF,IGF-land HGF after 24 hours' culture(P>0.05).3H-thy incorporation percentage(15.9±1.4,13.2±1.5),and concerntration of EGF [(722.9±55.4)ng/L,(498.2+41.5)ng/L]and IGF-1[(755.2±35.7)ng/L,(538.1±37.5)ng/L]in Group A and B were higher than that in group C after 72 hours(P<0.05).Especially the indexes above in group A were higher than that in group B(P<0.05).there was no significant difference in concerntration of HGF among the 3 groups after 72 hours(P>O.05).Conclusions These results imply that the regenerating hepatocytes contribute to the hyperproliferative state of co-culturing colon cancer cells.The mechanism maybe have relationship with EGF and IGF-1 high expression in colon cancer cells caused by growth signals coming from hepatocytes.     

VEGFR-32-Ig反义多肽的原核表达及生物活性鉴定

目的 将VEGFR-32-Ig的反义基因在体外进行原核表达，并将产物进行生物活性鉴定．方法 构建VEGFR-32-Ig反义基因的原核表达载体，经过原核表达、亲和层析及蛋白酶切后获得将VEGFR-32-Ig的反义多肽，用MTT法进行活性鉴定．结果 获得VEGFR-32-Ig的反义多肽，MTT法证明VEGFR-32-Ig的反义多肽具有较强生物学活性．结论 成功获得VEGFR-32-Ig的反义多肽，为研究和利用反义多肽提供了实验理论依据．     

PCR技术在赫坎按蚊复合体近缘种鉴别中的应用

目的应用聚合酶链反应（PCR）技术分析湖北省不同地区媒介按蚊的种型.方法采用传统的形态学方法和新建立的基因鉴别技术对现场捕获的按蚊分别进行形态特征鉴别和基因鉴别及比较.结果现场捕获181只按蚊,形态学确认176只为中华按蚊,而PCR鉴定172只为中华按蚊,另4只为八代按蚊;经形态学特征鉴别的5只嗜人按蚊中,PCR鉴别有4只为嗜人按蚊,另1只为八代按蚊.结论采用PCR基因鉴别技术能准确鉴别赫坎按蚊种团内中华按蚊、嗜人按蚊、八代按蚊等近缘种按蚊,较传统的按蚊形态学鉴别方法准确,适用于复合媒介地区的疟疾媒介调查和监测.     

视网膜母细胞瘤Rb50细胞株VEGFR-3表达的研究

目的:研究视网膜母细胞瘤细胞株VEGFR-3的表达,探讨视网膜母细胞瘤自身增殖的机制.方法:选择Rb50细胞株,通过S-P免疫组化法、RT-PCR法及PCR产物琼脂糖凝胶电泳等方法,研究Rb50细胞株VEGFR-3的表达情况.结果:S-P免疫组化法呈阳性结果,表明Rb50细胞株有VEGFR-3的表达,RT-PCR产物琼脂糖凝胶电泳表明Rb50细胞株有较高水平VEGFR-3 mRNA的存在.结论:视网膜母细胞瘤细胞株有VEGFR-3的表达,表明VEGFR信息通路对视网膜母细胞瘤生长、浸润的影响,除了与促进肿瘤血管及淋巴管新生,改善肿瘤组织微环境相关外,还与促进肿瘤细胞自身增殖有关.     

日本血吸虫病鼠肝卵冰冻切片免疫荧光法检测日本血吸虫病研究

为提高IFA法诊断血吸虫病的敏感性、特异性及实用价值,1986年我们对IFA法原有的抗原进行改进,采用日本血吸虫病鼠肝卵冰冻切片为IFA抗原。对58例基本消灭日本血吸虫病地区镜检孵化均阳性的病人血清,39例非疫区健康人血清进行IFA检测。同时对该抗原与原有成虫冰冻切片、肝卵石蜡切片两种抗原进行阳性检出率、抗原制备工艺流程和经济成本等几方面的比较。并设COP为试验对照。     

湖北省狂犬病流行病学分析

目的了解湖北省狂犬病的流行病学特点,及时发现和报告疫情,有效控制狂犬病。方法收集国家疾病监测信息网络直报的资料,湖北省全年24h狂犬病疫情报告资料,使用Excel软件进行统计分析。结果2001~2006年全省共报告狂犬病769例,发病率波动在0.032/10万~0.38/10万之间;秋冬季病例增多,发病人群以农民为主。结论应采取综合性狂犬病的预防措施,控制传染源,切断传播途径,暴露后的伤口应及时处理并及时接种狂犬疫苗和抗狂犬病血清。     

湖北省水痘流行病学分析

水痘（varicella，chickenpox）是由水痘带状疱疹病毒所引起的急性传染病，以较轻的全身症状和皮肤粘膜上分批出现的斑疹、丘疹、水疱和痂疹为特征，该病多见于小儿。为了解湖北省水痘的流行病特征，为预防控制水痘提供科学依据，对湖北省2005～2007年水痘疫情进行流行病学分析如下。     

靶向血管内皮生长因子-C的RNA干扰对裸鼠乳腺癌影响作用的实验观察

目的检测血管内皮生长因子-C（VEGF-C）的RNA干扰技术对裸鼠乳腺癌的影响作用，探讨RNA干扰对乳腺癌基因治疗中的有效性和相关机制。方法36只NOD-SCID小鼠随机分为空载体组12只，阴性短发夹RNA（ShRNA）对照组12只及VEGF-C ShRNA转染组12只。构建携带有绿色荧光蛋白报告基因的VEGF-C ShRNA质粒载体，并导入乳腺癌细胞MCF-7裸鼠皮下移植瘤，尾静脉注射VEGF-C ShRNA表达载体，观察其对乳腺癌生长的影响作用；酶联免疫吸附剂测定（ELISA）方法检测VEGF-C在肿瘤组织中的蛋白浓度；免疫组织化学分析肿瘤组织VEGFR-3阳性脉管密度。结果全身系统性给予VEGF-C ShRNA后，空载体组、阴性ShRNA对照组及VEGF-C ShRNA转染组肿瘤体积分别为（250±4）mm^3、（230±26）mm^3（P=0．2）、（97±25）mm^3（P〈0．05）；瘤组织中VEGF-C浓度在空载体组、阴性ShRNA对照组及VEGF-C ShRNA转染组分别为（496±7）pg／mg、（362±95）pg／mg（P=0．2）和（115±55）pg／mg（P〈0．05）；免疫组化分析VEGF-C ShRNA转染组肿瘤组织中VEGFR-3阳性脉管生成减少（P〈0．05）。结论VEGF-C的RNA干扰明显抑制了VEGFR-3阳性脉管的形成，抑制肿瘤的生长，有望通过RNA干扰技术实现乳腺癌的基因治疗。