两种术式治疗直肠前突的临床分析

目的治疗直肠前突的2种术式:经阴道修补直肠前突、经肛门切开黏膜间断缝合修补直肠前突并内外括约肌部分切断术进行疗效分析。方法将我科先后收治36例直肠前突患者随机分为2组,其中A组17例,采用经肛门切开粘膜间断缝合修补直肠前突并内外括约肌部分切断术;B组19例采用经阴道修补直肠前突术,对临床资料进行分析总结。结果两种术式疗效有统计学意义(P<0.05)。结论经肛门切开粘膜间断缝合修补直肠前突并内外括约肌部分切断术治疗直肠前突近远期疗效好。     

消痔灵双层注射直肠黏膜瘢痕固定肛门紧缩术治疗直肠脱垂16例

我科采用消痔灵双层注射直肠黏膜瘢痕固定肛门紧缩术治疗完全性直肠脱垂16例。取得满意疗效,报告如下。     

血液透析中腹痛原因的探讨

血液透析是终末期肾病的常见治疗方法之一。在血液透析过程中,并发症的发生相当常见,且十分复杂。在相关杂志中,对血液透析中腹痛并发症的报道少见,但其发生率在临床中仍占一定比重。为更好的防止并发症的发生,经过对本院2429例血液透析的回顾分析,共11次在透析过程中发生急性腹痛,发生率为0.45%。现报告如下。1　对象和方法1998年3月至1999年10月在本院进行血液透析的终末期肾病的病人共58例,其中有6例在透析过程中发生急性腹痛共11次。男性2人,女性4人。年龄30～70岁(平均年龄52.09岁)。以上病人均每周透析2次,每次透析4h,透析平均时间为1…     

骨髓间充质干细胞向软骨及骨分化：Wnt5a/PCP信号通路作用的研究与进展

BACKGROUND: Wnt5a is able to inhibit canonical Wnt signaling and activate non-canonical Wnt signaling pathway. In recent years, it has been found that non-classical Wnt5a/PCP signaling pathway mediated by Wnt5a plays an important role in the process of bone marrow mesenchymal stem cell proliferation and differentiation, but the underlying mechanism is unclear. OBJECTIVE: To summarize the progress in downstream effector molecules related to Wnt5a/PCP signaling pathway, and its roles in the chondrogenic and osteogenic differentiation of bone marrow mesenchymal stem cells. METHODS: A computer-based online search of CqVip, CNKI and PubMed databases between January 2000 and February 2016 was performed using the Chinese keywords of “BMSCs, Wnt signaling pathways, chondrogenic differentiation, osteogenic differentiation” and English keywords of “BMSCs, chondrogenic differentiation, osteogenic differentiation, Wnt, Fzd, Ror2, RhoA, ROCK, JNK”, respectively. Literatures related to bone marrow mesenchymal stem cell chondrogenic and osteogenic differentiation were selected. Finally, 43 eligible articles were included for analysis through excluding the old and repeated research. RESULTS AND CONCLUSION:Wnt5a, a representative protein in non-canonical Wnt signaling pathway, paticipates in the cytoskeleton, cell migration and cell polarization and other activities by mediating its downstream signaling molecules such as Fzd, Ror, RhoA, ROCK, JNK, thereby regulating its proliferation and differentiation. But it is unclear how Wnt5a/PCP participates in the bone marrow mesenchymal stem cell chondrogenic and osteogenic differentiation and how the downstream effector molecules interact or function independently, which requires further studies. 中国组织工程研究杂志出版内容重点：组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松;组织工程     

38例小儿急性氟乙酰胺中毒分析

当今灭鼠药在家庭中广泛应用,造成儿童误服致意外中毒,这是城乡儿童常见的急症之一。我院于１９９７～１９９９年共收治３８例氟乙酰胺中毒病例,现报道如下。 临床资料１　一般资料　本组病例中,男２４例,女１４例。年龄２岁以下４例,～４岁１６例,～６岁１２例,大于６岁６例。最小年龄１８个月,最大年龄１３岁。农村３１例,城镇７例。中毒途径均为误服。２中毒至入院时间　＜３０分钟４例,～１小时１０例,～３小时１６例,～５小时６例,５小时以上２例。３　临床表现　恶心呕吐３５例,阵发性抽搐３２例,头痛头昏２２例,腹…     

大鼠骨髓间充质干细胞最适培养基的筛选

  目的  探讨不同培养基对大鼠骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)生长的影响,筛选对大鼠BMSCs生长较适宜的培养基。  方法  采用全骨髓差速贴壁分离法从SD大鼠股骨和胫骨分离BMSCs,分别选用DMEM-LG、α-MEM、DMEM/F12三种培养基分离培养大鼠BMSCs。倒置相差显微镜下观察不同培养基对大鼠BMSCs形态均一化程度、克隆形成时间与第14天克隆形成数量、第1次传代时间、细胞增殖率以及细胞贴壁率等的影响;流式细胞术鉴定并观察不同培养基对大鼠BMSCs表面抗原表达的影响。  结果  与其他两组相比,体外DMEM-LG培养基培养的大鼠BMSCs形态均一、克隆形成时间和第1次传代时间短、克隆形成数量多达(14±2)个、细胞贴壁率高达(47.0±2.8)%;同时,BMSCs进入对数生长期仅需4 d,且平均增殖率最高,单位时间内平均扩增数量最多,3 d总扩增数量达(2.2~2.7)×105 mL-1;采用DMEM-LG、α-MEM、DMEM/F12三种培养基分离培养的细胞均为大鼠BMSCs,且不同培养基对大鼠BMSCs表面抗原表达无明显影响。  结论  DMEM-LG培养基较适合大鼠BMSCs的生长。     

Rac1参与调节IL-8诱导的内皮细胞迁移

研究Rac1是否参与调节IL-8诱导的内皮细胞迁移.采用Transwell小室迁移率分析法,考察不同Matrigel稀释比例以及IL-8不同作用时间对内皮细胞迁移的影响;同时用RT-PCR法检测Rac1 mRNA的表达.结果表明,Matrigel稀释比例为1:2时与比例为1:3、1:8时细胞迁移数量有显著性差异,而比例为1:4、1:5和1:6时与其他各组没有显著性差异;故选用Matrigel稀释比例1:4进行后期实验,随着IL-8作用时间的增加,内皮细胞迁移数量也逐渐增加;IL-8作用6h后,Rac1 mRNA的表达较其他各时间组稍强.以上结果提示,Rac1参与调节IL-8诱导的内皮细胞迁移,本实验为进一步研究Rac1在IL-8诱导内皮细胞迁移中的作用奠定基础.     

卵巢切除及雌二醇对大鼠腰椎松质骨微结构的作用及其生物力学性能的分析

应用micro-CT获得腰椎松质骨微结构的三维参数,分析卵巢切除术与雌二醇干预对大鼠松质骨微结构及整体骨生物力学性能的作用,初步讨论松质骨微结构的改变对生物力学性能的影响.6月龄未交配雌性SD大鼠30只,随机分成3组(每组10只):假手术对照组(Sham)、去卵巢组(OVX)和去卵巢 补充雌二醇组(EBT).术后相同条件饲养5个月,取第3腰椎进行生物力学压缩试验,第4腰椎行micro-CT扫描.结果表明,与相应的Sham组比较,OVX组的BV/TV、Tb.N均明显下降,Tb.Sp和SMI明显增高.EBT组的BV/TV、Tb.N和Tb.Th均大于OVX组,Tb.Th和SMI明显小于OVX组.骨力学性能检测显示OVX组腰椎松质骨E、Fmax和σmax均明显降低,而EBT组上述骨生物力学参数均明显改善.通过micro-CT获得的骨微结构参数并结合骨力学性能检测能为合理评价骨质疏松及抗骨质疏松药物药效研究提供较好的实验依据.     

实验动物与公共卫生安全的思考

开展实验动物研究是生命科学研究工作的基础和重要条件.是生物医学研究的重要手段,能直接影响许多领域课题研究成果的确立和课题质量水平的高低.同时,实验动物领域自身的发展和提高又会推动许多研究领域的发展.随着科学的发展,实验动物被广泛应用于生物、医药、化工、农业、畜牧、环保、军工、外贸、商检、宇航等领域,同时实验动物若出现意外也给公共卫生安全带来严重的隐患.因此,加强实验动物公共卫生安全管理至关重要.     

力学刺激调控骨髓间充质细胞向软骨的分化

BACKGROUND:Bone marrow mesenchymal cells are ideal seed cells for tissue engineering and cell therapy. We are seeking to improve the cartilage repair strategy based on bone marrow mesenchymal cells in vitro by use of natural mechanical transfer pathways. OBJECTIVE:To analyze the mechanical factors affecting the fate of bone marrow mesenchymal cells and utilize the mechanical strategy to induce chondrogenesis. METHODS:The first author retrieved the literature in CNKI, PubMed, Medline and Elsevier databases (1985-2015) with the key words of “mechanical regulation, bone marrow mesenchymal cells, bone marrow mesenchymal stem cells, chondrogenic differentiation, cartilage differentiation”. RESULTS AND CONCLUSION:Herein, we summarize some latest research results on the mechanical stimulus-induced chondrogenic differentiation of bone marrow mesenchymal cells, discuss the effects of different mechanical stimulations on the chondrogenic differentiation of bone marrow mesenchymal cells, as well as summarize the biological mechanism and signal pathways of the cells in response to mechanical stimulations in the process of chondrogenic differentiation. At last, this paper points out the future prospects of the key problems in this field, such as cartilage regeneration and repair strategy based on chondrogenic differentiation of bone marrow mesenchymal cells under mechanical stimulation.