中西医结合治疗老年溃疡性结肠炎的研究概况

目的老年溃疡性结肠炎患者逐渐成为溃疡性结肠炎患者中的第二大群体,国内外相关研究逐步展开,发病率的统计、临床特征的总结等方面趋于完善,本研究针对近5年老年溃疡性结肠炎的研究,以中西医结合治疗为出发点,探讨目前老年UC的治疗和研究现况。方法检索PubMed、Cochrane、CNKI等数据库中"老年溃疡性结肠炎(elderly/older-onset UC、UC in elderly patients/population)""中西医结合(Integrated Traditional Chinese Medicine&Western Medicine、Integrated/combined therapy)的随机临床试验,观察性研究、系统评价和/或荟萃分析及所选文章的参考文献、相关研究和评估中的参考文献。结果英文文献检索命中117条,筛选审查后选定27篇(含临床指南)。中文文献命中69条,筛查后选定18篇(含临床指南与专家共识)。所有纳入文献中,临床试验8篇(含2篇protocol),综述及观察性研究31篇。结论近5年国内对于老年UC研究并不多见,治疗方面临床试验以中成药联合西药为主,针对调节炎性因子及凝血功能。国外更多使用草药提取物联合用药治疗UC。     

针刺治疗阴虚型围绝经期综合征的临床观察

目的观察针刺治疗阴虚型围绝经期综合征的疗效。方法本研究采用随机对照的临床研究方法,观察针刺疗法治疗阴虚型围绝经期综合征的临床疗效。将阴虚型围绝经期综合征患者分为针刺组和更年安对照组,观察两组治疗前后的Kupperman指数,并进行疗效评价。结果针刺和更年安治疗均能改善阴虚型围绝经期综合征患者的临床症状,针刺组疗效优于更年安对照组。结论针刺治疗阴虚型围绝经期综合征疗效肯定。     

Introduction to HUA Xue-gui＇s Experience in Acupuncture Treatment of Tinnitus and Deafness

Doctor HUA Xue-gui, an associate chief physician in Shanghai Research Institute of Acupuncture and Meridian, has been engaged in clinical practice and scientific research of acupuncture for over 30 years. She has created a set of unique and complete acupuncture modalities for neurotic tinnitus and deafness. The authors are honored to have studied with Doctor HUA and have achieved a lot. Now, Doctor HUA＇s experience is summarized as follows.     

Influence of Moxibustion Serum on the Expression of Fas bcl-2 mRNA and Protein of EL-4 Lymphoma Cells

目的：观察小鼠艾灸血清体外对EL-4淋巴瘤细胞Fas、bcl-2mRNA及蛋白表达的影响。方法：采用小鼠艾灸血清培养EL-4淋巴瘤细胞24h后，应用原位杂交方法检测EL-4细胞Fas、bcl-2mRNA的表达，免疫细胞化学方法观察Fas、bcl-2蛋白表达。结果：与正常小鼠血清比较，艾灸血清培{f-EL-4细胞后，在bcl-2 mRNA及蛋白水平上，阳性细胞百分率均明显降低（P〈0．05），在FasmRNA及蛋白水平上，阳性细胞百分率均明显升高（P〈0．05）。结论：艾灸血清能够下调EL-4细胞bcl-2mRNA及蛋白的表达，上调FasmRNA及蛋白的表达。     

Effect of electro-acupuncture on matrix metalloproteinase in degenerated cervical intervertebral disc of rats

To observe the effect of electro-acupuncture on matrix metalloproteinase in degenerated cervical intervertebral disc rats.MethodsThe rat model of cervical intervertebral disc degeneration was induced by unbalanced dynamic and static forces,then the rats were randomly allocated to model group,electro-acupuncture groupⅠ(acupoints Dazhu(BL 11) and Tianzhu(BL 10) were electro-acupunctured),and electro-acupuncture group Ⅱ(acupoints Dazhu (BL 11),Tianzhu(BL 10)and Shenshu(BL 23) were electro-acupunctured),with the normal rats as control.then the HE staining method was adopted to observe the morphological change of cervical intervertebral disc and the immunohistochemical staining method was used to detect the marx metalloproteinases-1(MMP-1) and matrix metalloproteinases-3(MMP-3) in cervical intervertebral disc.ResultsThe morphological observation showed that after electro-acupuncture treatment,the degrees of cervical intervertebral disc degeneration in electro-acupuncture groupⅠand electro-acupuncture group Ⅱ were alleviated,and the electro-acupuncture group Ⅱ was superior to electro-acupuncture group Ⅰ.The expressions of MMP-1 and MMP-3 in degenerated cervical intervertebral disc were increased(P＜0.01),and after electro-acupuncture treatment,the expressions of MMP-1 and MMP-3 in degenerated cervical intervertebral disc were decreased,especially the electro-acupuncture group Ⅱpresented the better effect as compared with electro-acupuncture group Ⅰ(P＜0.05).ConclusionsElectro-acupuncture at acupoints Dazhu (BL 11) and Tianzhu (BL 10) had a certain therapeutic effect on cervical intervertebral disc degeneration,and in combination with acupoint Shenshu(BL 23),this therapeutic effect could be enhanced,its action mechanism might be that electro-acupuncture can reduce the expressions of MMP-l and MMP-3 in degenerated cervical intervertebral disc,inhibit the degradation of matrix in intervertebral disc.so as to relieve cervical intervertebral disc degeneration.     

Treatment of 102 Cases of Tourette＇s Syndrome with Acupuncture plus Herbal Medicine

102例抽动-秽语综合征患者分为肝肾阴虚型、痰湿阻滞型和脾胃虚弱型三型.采用针刺,耳穴贴压,拔罐和中药相结合的方法进行治疗.结果显效30例,有效61例,无效11例,总有效率89.2%.治疗过程中未见任何毒副作用.     

针刺对围绝经期大鼠卵巢颗粒细胞Fas、Bcl-2 mRNA表达的影响

目的从细胞凋亡角度研究针刺治疗围绝经期综合征的作用机制。方法将围绝经期大鼠随机分为围绝经期组、针刺组和药物组,并设青年对照组。围绝经期组大鼠不治疗,作为空白对照;针刺组大鼠针刺肾俞、足三里、三阴交治疗;药物组大鼠采用更年安药物治疗;青年对照组大鼠不治疗。应用原位杂交法检测卵巢颗粒细胞Fas、Bcl-2 mRNA表达,并进行图像分析。结果与青年对照组比较,围绝经期组大鼠卵巢颗粒细胞Fas mRNA表达明显升高(P<0.01),Bcl-2 mRNA表达明显降低(P<0.01);针刺治疗后围绝经期大鼠卵巢颗粒细胞Fas mRNA表达明显降低(P<0.01),Bcl-2 mRNA表达明显提高(P<0.01)。结论针刺能够下调围绝经期大鼠卵巢颗粒细胞Fas mRNA表达,上调Bcl-2 mRNA表达。     

耳穴贴压结合拔罐治疗女性更年期综合征28例

女性更年期综合征是妇女在绝经前后出现的一组症候群 ,临床主要表现为植物神经功能紊乱的症状 ,如潮热多汗、烦躁易怒、心悸失眠、头痛口干等。笔者采用耳穴贴压结合拔罐疗法治疗该病 2 8例 ,疗效满意 ,现总结如下。1　一般资料43例均为门诊病人 ,随机分为治疗组 2 8例和对照组 1 5例。年龄在 43岁～ 5 7岁之间 ;病程最长 6年 ,最短 1个月 ;绝经者 2 4例 ,未绝经者 1 9例。两组患者一般情况相似 ,具有可比性。2　诊断标准依照《实用中西医结合诊断治疗学》[1]中有关更年期综合征的诊断标准并结合改良的 Kupperman指数评定法 [2 ] ( Kupperm…     

Experimental research of electroacupuncture on ERK phosphorylation in rats of hepatofibrosis

目的：探讨电针肝俞、足三里对肝纤维化模型大鼠ERK蛋白激酶磷酸化的干预作用，从分子水平研究电针防治肝纤维化的作用机制。方法：将40只wistar雄性大鼠随机分为4组，正常组、模型组、电针组和药物组。采用30％的四氯化碳橄榄油皮下注射复合含10％乙醇的自来水饮用诱导大鼠肝纤维化模型，电针组选取肝俞、足三里进行电针刺激，药物组采用秋水仙碱灌胃治疗；采用天狼猩红染色方法观察大鼠肝组织胶原增生情况；应用Western—blotting方法检测肝组织细胞外信号调节激酶（ERK）和磷酸化ERK的表达。结果：与正常组比较，模型组肝组织胶原沉积明显增加，磷酸化ERK蛋白的表达量明显上升（P〈0．01）；与模型组比较，电针组和药物组大鼠肝组织胶原沉积明显减少，磷酸化ERK蛋白的表达量明显下降（P〈0．01）；电针组和药物组比较，上述指标差异均无统计学意义。结论：电针可能通过下调肝组织ERK蛋白的磷酸化，进而阻抑MAPK／ERK信号转导通路，抑制细胞外基质的合成，减少胶原沉积，从而起到防治肝纤维化的作用。     

Effect of Acupuncture-moxibustion on TNF-α, sTNFR-I and sTNFR-Ⅱ of Rats with Crohn＇s Disease

目的：观察针灸对克罗恩病大鼠血清TNF—α、sTNFR—I、sTNFR-II的影响。方法：采用TNBs制备大鼠克罗恩病模型，随机分为模型组、隔药灸组、电针组，并与正常大鼠作对照，采用HE染色观察结肠黏膜组织病理学变化，应用ELISA方法检测血清TNF-α、sTNFR-Ⅰ、sTNFR—Ⅱ的含量。结果：与正常组比较，CD大鼠血清TNF—α显著升高、sTNFR-Ⅰ和sTNFR-Ⅱ无显著变化。经隔药灸和电针治疗后血清TNF—α水平显著降低，sTNFR-Ⅰ和sTNFR-Ⅱ无显著变化。CD大鼠结肠组织的炎症病变和异常的组织结构在隔药灸、电针治疗后得到明显改善。结论：隔药灸与电针均能够显著降低血清TNF-α的含量，该作用可能是隔药灸与电针治疗CD的作用机制之一。