帕金森氏病和肌营养不良症（Erb型）中线粒体DNA的部分缺失

用ＰＣＲ技术对１２例神经肌肉性疾病患者的１６个标本进行了线粒体ＤＮＡ缺失情况的研究。发现２例帕金森氏病患者的肌肉组织以及其中１例的外周血和另１例肌营养不良（Ｅｒｂ型）患者的肌肉组织中线粒体ＤＮＡ至少有５２６ｂｐ的缺失。还对线粒体ＤＮＡ的部分缺失与疾病发生的关系进行了讨论。     

调味品中氯丙醇气相色谱法测定

毒理学研究表明，氯丙醇对人体的肝、肾、生殖系统、神经系统有一定损害，且有潜在的致癌性。目前调味品中氯丙醇（3-MCPD、1，3-DCP）严格控制限量，规定3-MCPD〈1mg／L、1，3-DCP〈50μg／L。本采用电子捕获检测器（GCECD），同时快速测定1，3-DCP和2，3-DCP。该方法前处理通过蒸馏、萃取、氮气吹扫，有效对样品提取和净化，方法干扰少，线性范围1，3-DCP和2，3-DCP均为0．05～25．0μg／ml；最低检出限为0．025μg／ml；回收率为93．1％～111％；重复性RSD〈10％；显示出良好的准确度和精密度，有较强的适用性。     

超高效液相色谱串联质谱法测定消毒产品中违禁抗真菌药物

摘要 目的 建立测定消毒产品中违禁添加抗真菌药物成分的方法。 方法 采用超高效液相色谱串联质谱法，对消毒剂中添加的9种违禁抗真菌药物成分做出测定方法的建立和验证。 结果 以1%甲酸乙腈溶液为流动相、用PSA去除杂质和外标法定量。酮康唑、氟胞嘧啶、氟康唑、灰黄霉素、联苯苄唑、硝酸益康唑、硝酸咪康唑、盐酸萘替芬和克霉唑9种抗真菌药物成分都确定了线性范围和最低检出限，线性相关系数(r2)均＞0.99；加标回收率为72.0%～106.4%，相对标准偏差为2.52%～6.15%。 结论 该方法适用于各种剂型消毒产品中多组分抗真菌药物的检测，为消毒产品的检测和监管提供了技术支持。     

转移性肾癌的临床治疗研究进展

近年来,随着靶向治疗的出现与发展,逐渐代替了以前单纯的干扰素α和白细胞介素-2的免疫治疗.目前已有7种靶向治疗试剂获得美国食品与药物管理局(FDA)的批准,用于临床晚期转移性肾癌的治疗.这7种药物分别为:舒尼替尼、索拉非尼、阿西替尼、帕唑帕尼、贝伐单抗、依维莫司和坦斯莫司.有些新药尚在试验阶段.这些药物的作用原理主要是抑制血管内皮生长因子与哺乳动物类雷帕霉素靶蛋白(mTOR)通路,从而达到治疗肿瘤生长的目的.因此,治疗效果也取决于是否药物能有效抑制肿瘤血管的形成或有效阻断mTOR通路.较比免疫治疗,靶向治疗的效果明显提高.本文就这些免疫治疗和靶向治疗药物作一综述,为临床治疗肾癌在选取药物方面提供参考.     

掩饰性治疗对成人骨性Ⅱ类错患者上气道及周围结构影响的研究

目的研究拔牙与非拔牙矫正对成人骨性Ⅱ类错气道及周围结构的影响,并分析可能的原因。方法选择36例成人骨性Ⅱ类错正畸结束病例,拔牙组20例,非拔牙组16例。用正颌外科模拟预测系统(CASSOS)软件测量患者治疗前后X线头颅侧位片,并对数据进行配对t检验。结果拔牙组与非拔牙组的腭后气道(Phw1-psp)与舌后气道(Phw2-Tb)在正畸前后均无显著变化(P>0.05)。非拔牙组的前颌间距离(Ant.ln Mx.Ht)与颌间面积(ln Mx Area)有所增加,差异有统计学意义。结论成人骨性Ⅱ类错畸形的掩饰性治疗,不论是否减数,在下前牙不过分内收的情况下,气道在矢状向的变化并不显著。     

逆转录—多聚酶链反应检测柯萨奇B组病毒

研究目的用分子生物学方法对柯萨奇Ｂ组病毒（ＣＢＶ）进行检测并探讨与临床症病的关系。研究方法对我院２９８例疑似ＣＢＶ感染的患儿，采用特异性引物进行逆转录－多聚酶链反应（ＲＴ－ＰＣＲ）检测ＣＢＶ。结果检出ＣＢＶ阳性１２７人，阳性率４２．６％。感染与年龄有关，夏秋季多见。８０例健康儿童中，阳性５人，占６．２５％。２０例ＲＴ－ＰＣＲ检测阳性标本用ＥＬＩＳＡ法检测，１７例阳性。结论ＲＴ－ＰＣＲ可快速、敏感、准确地检出标本中的ＣＢＶ。此病毒可侵犯多种器官，引起各种临床症病，有较强的传染性。     

毒鼠强浓度在人体内变化规律研究

Objective To study the variation of blood concentration of tetramine in human body with acute tetramine intoxication treated with three different protocols and the levels in human body after discharge from the hos-pital. Methods The blood concentration of tetramine was determined by gas chromatography-mass spectrometry (GC-MS). All 101 patients of acute tetramine intoxication were divided into 3 groups (routine comprehensive treat-ment group,hemoperfusion group,blood transfusion group) according to their blood concentration of tetramine and clinical symptoms. The patients were followed up to monitor the tetramine levels in a year. Results The level of tet-famine in blood was decreased from 33.0(1.7～115.0)μg/L to 18.0(0.3～47.6)μg/L in routine comprehensive treatment group. The total decrement was 45.5 %. The level was decreased from 108.0 (54.0～290.0)μg/L to 26.0 μg/L in hemoperfusion group. The total decrement was 75.9%. The decrement was 20.0%～45.0% after each he-moperfusion. The level was slightly high after 24 h of hemoperfusion. The total decrement in blood transfusion group was 33.5%～60.0%. The level was <0.3μg/L in all 25 out-patients 1 year after their intoxication. Conclusion Routine comprehensive treatment,hemoperfusion,blood transfusion are effective in the treatment of acute tetramine intoxication. The degradation of tetramine in human body is slow.     

毒鼠强浓度在人体内变化规律研究

Objective To study the variation of blood concentration of tetramine in human body with acute tetramine intoxication treated with three different protocols and the levels in human body after discharge from the hos-pital. Methods The blood concentration of tetramine was determined by gas chromatography-mass spectrometry (GC-MS). All 101 patients of acute tetramine intoxication were divided into 3 groups (routine comprehensive treat-ment group,hemoperfusion group,blood transfusion group) according to their blood concentration of tetramine and clinical symptoms. The patients were followed up to monitor the tetramine levels in a year. Results The level of tet-famine in blood was decreased from 33.0(1.7～115.0)μg/L to 18.0(0.3～47.6)μg/L in routine comprehensive treatment group. The total decrement was 45.5 %. The level was decreased from 108.0 (54.0～290.0)μg/L to 26.0 μg/L in hemoperfusion group. The total decrement was 75.9%. The decrement was 20.0%～45.0% after each he-moperfusion. The level was slightly high after 24 h of hemoperfusion. The total decrement in blood transfusion group was 33.5%～60.0%. The level was <0.3μg/L in all 25 out-patients 1 year after their intoxication. Conclusion Routine comprehensive treatment,hemoperfusion,blood transfusion are effective in the treatment of acute tetramine intoxication. The degradation of tetramine in human body is slow.