鸡贫血病毒VP 3基因的克隆及其体外凋亡诱导效应的研究

用PCR方法扩增了鸡贫血病毒标准株的vp3基因,并将其克隆于真核表达载体pcDNA3上,构建了重组体pcDNA-vp3.经酶切鉴定及测序分析表明,该片段和预期相符.在体外利用LipofectAMINETM介导的基因转染,将pc-DNA-vp3、pcDNA3分别转入肝癌细胞系HepG2和二倍体肝细胞系L-02中,转染后的RT-PCR结果证实vp3基因在细胞中得到了表达.同时利用筛选稳定表达细胞株的技术和原位细胞凋亡检测法,证明了鸡贫血病毒是以凋亡的方式诱导细胞死亡,并且只诱导癌细胞的凋亡,而不诱导正常或二倍体细胞死亡.表明鸡贫血病毒vp3基因很可能成为一种极具潜力的抗肿瘤生物制剂.     

以效益/费用比评估幽门螺杆菌根除方案

目的 对幽门螺杆菌根除方案进行综合评估,为临床医师提供选择参考。方法 对近5年来国内医学期刊报道的数十个幽门螺杆菌(HP)根除方案以HP根除率、疗程(以抗菌药物使用时间为准),不良反应发生率及其严重程度,服药方法等项作为效益(Utility,U)的主要评分指标,并计算抗HP治疗费用(Cost,C)。按U/C比值的大小判定优劣。结果 以方案19(庆大霉素16万U,tid;雷尼替丁0.3g,bid;阿莫西林0.75g,bid;胶态次枸橼酸铋1.0g,qid)和方案20(以四环素0.75g,qid,代替方案19中的阿莫西林,余同方案19)两个二日疗法的四联方案为最佳。结论 效益/费用为一较合理、实用的评估方法,据此筛选出疗程超短、高效、无严重不良反应而又价廉的二日疗法四联方案,但尚待更多病例验证。     

高血压患者脉压差与左心功能的关系

INTRODUCTIONRecentlymanyreportsrevealedpulsepressureiscloselycorrelatedwithoccurrenceanddeathofcoronarydisease犤１－４犦．Butitisnotclearwiththerelationshipbetweenpulsepressureandventricularfunctionofhypertensionpatients．Toinvestigateeffectofpulsepressureonventricularfunctionofhypertensionpatients，weexploredrelation－shipofpulsepressureandventricularfunction．MATERIALSANDMETHODSMaterials４２hypertensionpatientswerereceivedfrom１９９５to２０００toourhospitalanddiagnosedashypertens…     

Experimental study on the antitumor effect of chicken anemia virus vp3 gene against liver carcinomain vivo

Summary In order to testify the antitumor effect, especially its effect against liver carcinomain vivo, of VP3 protein, one kind of protein coded by chicken anemia virus, recombinants pcDNA-vp3 containing chicken anemia virus vp3 gene, and control vector pcDNA3 were mixed with murine liver carcinoma cell lines H22 respectively. The mixture was injected subcutaneously into Balb/C mice. Some days later, the mice were killed and the solid tumor weighed. The antitumor efficiency was evaluated. The manners of VP3 proteinin vivo, inducing tumor cell death were identified by using TUNEL assay. All the results suggested that the injection of pcDNA-vp3 and H22 mixture resulted in a significant reduction of tumor growth in mice when compared with the results of control groups. TUNEL assay revealed that VP3 induced apoptosisin vivo. All these indicated that CAV vp3 might be a potential new gene in reducing the growth rate of tumor cells in liver carcinoma or in other kind of solid tumorsin vivo. SHEN Zhifa, male, born in 1971 Lecturer     

肥胖对高血压病患者颈动脉粥样硬化的作用

目的肥胖是心脑血管疾病的危险因素,而关于肥胖与颈动脉粥样硬化的关系尚不清楚。方法通过多普勒超声检查高血压病患者颈动脉粥样硬化的方法,观察了82例高血压病患者体重指数与颈动脉粥样硬化斑块的发生率及内中膜厚度的相互关系。结果体重指数高的患者其颈动脉粥样硬化斑块的发生率及内中膜的厚度也高(P<005,P<001)。结论体重指数与颈动脉粥样硬化斑块和内中膜厚度相关,肥胖可能参与动脉粥样硬化的形成和发展。     

鸡贫血病毒VP3基因体内抑瘤效应的实验研究

目的 观察鸡贫血病毒（chicken anemia virus,CAV)VP3基因的体风抑瘤效应。方法 分别将PCDNA-VP3（含VP3基因的真核表达载体），PCDNA3（空载体）和小鼠腹水型肝癌细胞株H22混合后，接种于BALB/c小鼠皮下，几天后，处死动物，取肿瘤组织称重，比较试验组和对照组瘤重差异，确定VP3基因的抑瘤效应，TUNEL法鉴定VP3在体内诱导肿瘤细胞死亡的方式。结果 注射VP3基因的试验组肿瘤生长率明显低于注射空载体的对照组。TUNEL试验的结果也表明，鸡贫血病毒VP3蛋白在体内调亡方式诱导肿瘤细胞死亡。结论 预示VP3基因可能在抑制肝癌细胞生长方面有一定的疗效。     

关节镜术后用冰生理盐水持续冲洗在膝骨性关节炎治疗中的临床应用

目的探讨关节镜术后用冰生理盐水持续冲洗在膝骨性关节炎治疗中的疗效。方法回顾性分析我院2009年11月～2012年10月收治的178例运用关节镜术治疗膝骨性关节炎患者的临床资料。结果实验组的膝关节功能优良率、肿胀程度、自主活动度皆优于对照组（P〈0.05）。结论关节镜术后用冰生理盐水持续冲洗在膝骨性关节炎治疗过程中可取得很好的效果,值得临床推广。     

腰椎后路椎弓根钉内固定配合自体颗粒骨椎间打压植骨术治疗退行性腰椎病变

目的探讨腰椎后路椎弓根钉内固定配合自体颗粒骨椎间打压植骨术治疗退行性腰椎病变的临床疗效。方法对54例退行性腰椎病变患者采用腰椎后路椎弓根钉内固定配合自体颗粒骨椎间打压植骨术治疗,通过随访进行疗效评定。结果 54例随访6～30个月,平均14个月。术后所有患者植骨处均骨性愈合,复位无丢失,无内固定松动、断裂。临床疗效综合评价:优32例,良13例,可7例,差2例,优良率83.3%。结论腰椎后路椎弓根钉内固定配合自体颗粒骨椎间打压植骨术是治疗退行性腰椎病变的有效方法。     

Comparisons in finite element analysis of minimally invasive,locking,and non-locking plates systems used in treating calcaneal fractures of Sanders type Ⅱ and type Ⅲ

Background Calcaneal fractures are relatively common but complex,while the treatment of these fractures is still controversial.The objective of this study was to compare the biomechanical stability of minimally invasive,locking,and non-locking plating systems used for fixing Sanders type Ⅱ and type Ⅲ calcaneal fractures.Finite element analysis was used for comparing the three types of plating systems.This study helped in guiding the clinical management of calcaneal fractures.Methods The CT data acquired from a volunteer was used to construct a finite element model of calcaneus.The fracture lines were made according to Sanders type Ⅱ （A,B,C） and type Ⅲ （AB,AC,BC） and six calcaneal fractures models were constructed accordingly.Three fixation methods used in clinical treatment were simulated by design tools （minimally invasive plating with percutaneous screws,Y-size non-locking plating,and locking plating） and then fixed to these fracture models with the help of clinical principles.The single minimally invasive plating fixation was also tested to compare the function of percutaneous screws.Then,the neutral force was applied on established methods.The displacement and presses distribution condition in various plating models were analyzed and compared.Results The levels of displacement and compressive press on the bone around fixation or strains on plating were within the physiological limit,indicating that the three methods were suitable for application.Compared with the locked and unlocked plates,the minimally invasive plating caused a relatively lower displacement without the high stress in bone or fixation.The minimum stress in fixation was observed in locking plate as the distribution of stress was concentrated in the anterior part of locking plate.Conclusions The minimally invasive plating method could be a better alternative method in treating Sanders type Ⅱ and type Ⅲ calcaneal fractures.The observed stiffness of locking plate was not obviously stronger than unlocked plate.