REGULATING FUNCTION OF COENZYMIZATION AND DECOENZYMIZATION OF THE LACTATE DEHYDROGENASE ISOZYMES IN THE MOUSE TISSUES DURING HYPOXIA

Objective. To study the characteristics of changes of LDH enzyme patterns of mice under slight hypoxia.Methods. Mice treated with artificial hypoxia, various tissues were made for the test of LDH enzymatic activity by the specific staining technique. LDH (1 -5) relative percentage enzymatic activity (RPEA) were measured with CS-910 dual-wavelength thin layer chromatography scanner.Results. The RPEA of LDH isozymes of various tissues after slight hypoxia shifted to the isozymes LDH1 and LDH2, whose principal subunits are H subunits, and the RPEA of LDH,(H4), LDH2(H3M) increased, while RPEA of LDH5(M4) in various tissues decreased prominently except the cardiac muscle, and that of LDH4(HM3) decreased as well. After polyacrylamide gel electrophoresis (PAGE) of the hypoxia treated cardiac muscle specimen was made, activity subbands originated regularly in the isozyme patterns of LDH, with the regularity of LDH1 (0 subband), LDH2 (0-1 subbands), LDH3 (0-2 subbands), LDH4 (1-3 subbands), LDH5 (2-4 subbands).     

缺氧处理小白鼠乳酸脱氢酶同工酶的辅酶化和去辅酶化调控作用

研究生物缺氧应激反应调空模式小白鼠轻度缺的氧心肌乳酸脱氢酶谱变化特点。方法以小白鼠心肌为材料，人工缺氧，乳酸脱氢酶酶活性特异染色。ＣＳ－９１０型双波长薄层层析扫描仪定量测定ＬＤＨ相对百分含量。     

社区“以医助养”模式对老年人健康管理的影响观察

目的:社区采用以医助养模式对老年健康管理效果影响分析。方法:选取2016年10月-2017年10月本社区糖尿病和高血压132例老年患者,按随机数字表法分成两组,对照组予社区常规照顾,研究组予以医助养模式,分析两组服务依从性及自我效能、健康指标与社会支持情况。结果:研究组社区就诊、体检、健康教育等社区服务依从性依次为60例、39例、63例及53例比对照组多,比较差异具有统计学意义(P0.05);研究组自我效能(6.56±1.29)分及社会支持(41.62±3.69)分等健康管理指标比对照组高,比较差异具有统计学意义(P0.05)。结论:社区应用以医助养模式能提高老年人社区服务依从性,有效改善健康管理效果。     

介绍一种细胞色素C新的制备方法

<正> 细胞色素是包括多种含铁卟啉基团的电子传递蛋白的总称,只存在于需氧细胞中,以心肌和醇母细胞含量较高。细胞色素C(cyto-chrome C)是细胞色素的一种。是呼吸链中的一个重要成员。每分子细胞色素C含有一个血红素和一条多肽链。细胞色素的氨基酸顺序及含量都有一定差异。一般氨基酸残基数目在103～111之间;其等电点为10.7,含铁量为0.38％～0.43％,分子量为12000～13000。本文介绍一种新的细胞色素C的制备方法。提取:称取500g肌肉糜,放入500ml事先用硫酸(3mol/L硫酸25ml)酸化的