不同导联系统记录体表心室碎裂电位效果对比研究

本文分别应用标准１２导联系统，Ｆｒａｎｋ正交导联系统和改良Ｆｒａｎｋ正交导联系统记录健康人３６例、陈旧性心肌梗塞４２例的心室碎裂电位，通过分析这些导联系统中相互垂直或近似垂直的导联上ＶＦＰ的形态，数量关系，发现改良Ｆｒａｎｋ正导联系统记录ＶＦＰ较其它导联系统更科学，合理，且操作方便。     

大鼠海马CA1区酶组织化学研究

本文用组织化学方法,选择能反映神经组织糖代谢三个途径的关键酸及碱性磷酸酶,对正常SD系大鼠海马酶活性进行半定量研究.结果CA1区锥体层琥珀酸脱氢酶(SDH)及细胞色素氧化酶(CCO)呈轻度反应,葡萄糖-6一磷酸脱氢酶(G—6—PD)及乳酸脱氢酶(LDH)呈强阳性.腔隙分子层LDH、G—6—PD呈轻度活性,CCO、SDH呈强阳性.CA1区锥体层碱性磷酸酶(AKP)活性最弱.讨论了酶活性不同与记忆的关系及临床意义.     

Expressions of resistance and cross-resistance in teniposide-resistant L1210 cells

Summary Resistance to teniposide (VM-26) by VM-26 selected resistant L1210 cells in culture was attributed to alterations in the flux of VM-26 across the plasma membrane and to functions of homogeneously staining regions that appeared on one or more chromosomes. In the present study, electrophoresis of membrane-cytosol fractions of these resistant sublines demonstrated a protein band, M_r 22 kd, that was not evident in similar fractions of drugsensitive L1210 cells or three revertant sublines. The distribution of this protein among various cellular fractions could be altered by manipulation of the concentration of calcium ions. A representative subline, LIa5 M, was observed to have vesicles that reacted with Sudan black B stain, an indication of altered lipid metabolism. The LIa5 M subline was cross-resistant to etoposide, vincristine, doxorubicin, amsacrine, and actinomycin D. Concentrations of VM-26 that inhibited cell division to the same extent caused an accumulation of fewer cells in the G₂ stage of cell division in LIa5 M cultures than in L1210 cultures. These observations indicate that the LIa5 M subline expressed multiple drug resistance, as well as changes in the expression of cytotoxicity to VM-26.This investigation was supported by Research Project Grant CA 35319, by Cancer Center Support (CORE) Grant CA 21765 from the National Cancer Institute, and by the American Lebanese Syrian Associated Charities.Recipient of research support from a training grant, American Cancer Society Grant IN-85-06, to the University of Tennessee Center for the Health Sciences     

不同教学方法对大学生课堂学业情绪的影响

目的 了解教学方法对大学生学业情绪的影响情况.方法 对湖南某高校大一4个教学班168名被试,采用教育实验法探讨了活动教学法、讲授教学法和讨论教学法对大学生课堂学业情绪的影响情况.结果 大学生课堂学业情绪的得分在3种不同教学方法下存在显著差异(F=3.12～27.38,P＜0.05),学生在活动课体验到了更多的积极情绪和更少的消极情绪.结论 活动教学法有利于促进大学生课堂学业情绪的发展.     

HLA-Ⅰ类基因分子多态性与AIDS

人类对HIV的易感性及HIV/AIDS患者的疾病进展速度存在个体和种族差异,表现在多数HIV感染者在感染后2～10年内进展为AIDS;但有1%～5%的HIV感染者在感染后10年内疾病没有进展;更有一部分人,因为吸毒、性交等高危因素,长期频繁暴露于HIV,却没有被感染[1～3].近年来,遗传免疫学研究已经发现了一系列影响HIV/AIDS易感性的宿主遗传因素(图1),这些遗传特质主要涉及了2个环节:①编码辅助受体及趋化因子的基因,其多态性影响了HIV进入靶细胞;②编码产物参与机体针对HIV的获得性免疫反应的基因,其中尤其以HLA-Ⅰ类分子多态性对HIV/AIDS易感性的影响为研究热点(图1).本文将对近年来关于HLA-Ⅰ类分子对AIDS影响的研究进展作一总结.     

冠心病生存质量与中医证候轻重的相关性分析

目的研究分析冠心病患者的生存质量与中医证候轻重的相关性。方法应用已构建的冠心病中医证候疗效综合评价体系作为评价工具,冠心病患者入院后3d内与出院前3d填写生活质量表（SF-36）与中医证候临床观察表。以入出院生存质量差值与入出院证候轻重各指标差值进行比较。结果生存质量评分与证候综合疗效评价体系的各个层面均存在相关关系。在11个基本证候层面中,水湿证、气滞证、实热证与生存质量有显著的相关性;在实证与虚证层面,虚证与生存质量评分的相关性较为显著;在生存质量与证候总分之间,有显著的负相关关系（相关系数-0.939）。结论在冠心病中医综合疗效评价指标体系中,中医证候在各个层面与生存质量存在相关关系,且与证候总评价有较强相关性。     

Osteoporosis knowledge,self-efficacy,and health beliefs among Chinese individuals with HIV

Summary

The worldwide uptake of FRAX is described.

Introduction

The aim of this report was to determine the usage of FRAX worldwide over a 1-year period from 1 May 2012.

Methods

The number of FRAX calculations from each country was assessed over a 1-year period and expressed as calculations per million of the population aged 50 years or more. Countries were colour coded according to usage to populate a world map.

Results

Over the index year, there were estimated to be 2,391,639 calculations sourced from 173 counties. Uptake was high in North America, the Antipodes and most countries of Europe; intermediate in Latin America and the Middle East; and very low in Africa and much of South East Asia.

Conclusions

It is expected that the comparative data will encourage the development of new FRAX models and the uptake of FRAX into assessment guidelines.     

复方甘草酸苷胶囊治疗318例皮肤病临床观察

目的探讨复方甘草酸苷胶囊在皮肤科的临床应用效果。方法观察318例皮肤病患者口服复方甘草酸苷胶囊的临床疗效,一次2片,一日3次,饭后服用,14~21d为1个疗程,治疗期间停用其他内服和外用药物。结果复方甘草酸苷胶囊治疗斑秃有效率为86.00%,治疗皮炎有效率为90.00%,治疗湿疹有效率为94.00%,治疗荨麻疹有效率为82.00%,治疗玫瑰糠疹有效率90.00%,治疗皮肤瘙痒症有效率为86.00%,治疗过敏性紫癜有效率为93.33%,治疗银屑病有效率为93.75%,治疗痒疹有效率为83.33%,治疗扁平苔癣有效率为70.00%。结论复方甘草酸苷胶囊治疗318例皮肤病取得良好效果,费用低廉,适合基层医院推广运用。     

灰绿黄堇生物总碱抗炎镇痛作用的研究

目的研究灰绿黄堇生物总碱的药理活性及毒性,为进一步开发新药提供药理学依据。方法对实验鼠分别以二甲苯、琼脂致肿,观察灰绿黄堇的抗炎作用;采用扭体法观察灰绿黄堇的镇痛作用;用改良寇氏法计算半数致死量(LD50)及95%可信限。结果灰绿黄堇生物总碱制剂小鼠灌胃给药能抑制二甲苯所致耳肿胀和琼脂所致足肿胀,并能抑制酯酸所致小鼠扭体反应。其LD50为1.833g·kg-1,95%可信限为1.18~2.06g·kg-1。结论灰绿黄堇总生物碱具有抗炎和镇痛作用。     

震动眼震试验在眩晕诊断中的应用

目的:观测震动眼震(VIN)在眩晕诊断中的临床意义。方法:健康志愿者30例作为对照组,单侧前庭周围性疾病眩晕缓解期患者67例为实验组,分别进行震动试验(VT)和摇头试验(HST),观察眼震诱发情况。眩晕患者同时还进行冷热试验(CT)。结果:实验组VT中52例(77.6%)出现VIN,主要是水平眼震,其中44例VIN方向朝向健侧,梅尼埃病和前庭神经元炎患者分别有2例、6例VIN方向朝向患侧;引出摇头眼震(HSN)46例(68.7%),CT存在一侧半规管反应减退(UW)54例(80.6%)。对照组均无VIN和HSN。结论:VIN试验具有较高的敏感性,能够为评定前庭损伤侧别提供有益信息,安全性强、患者无痛苦、简便、易于推广,适合各级眩晕门诊的快速筛查。