内皮抑素在大鼠腹膜的表达及其与腹膜血管新生的关系

Objective To investigate the expression of endostatin (ES) in rat peritoneum and its association with peritoneal neoangiogensis. Methods Thirty-two male SD rats were randomly divided into 4 groups: normal control rats (C group), renal failure without PD rats (non-PD group), rats dialyzed with 1.5% PD solution (1.5% PD group) and 4.25% PD solution (4.25% PD group). After regular PD for 28 days, mRNA and protein expression of ES in peritoneal tissues of each group were detected by RT-PCR and immunohistochemistry respectively. Microvessel density (MVD) of peritoneal tissue was assessed using immunohistochemistry with CD34 monoclonal antibody. Results ES mRNA was expressed in each group, 0.47±0.05 in C group, 0.45±0.04 in non-PD group, 0.46±0.04 in 1.5%PD group, 0.47±0.03 in 4.25%PD group, and no significant differences were found among groups. Score of ES protein expression was O in C group, 2 in non-PD group, 4 in 1.5%PD group, and 9 in 4.25%PD group. MVD was 3.13±1.13 in C group, 5.13±1.14 in non-PD group, 9.00±1.51 in 1.5%PD group, 10.75±1.83 in 4.25%PD group, and significant differences were found among groups. Conclusion Uremia circumstance and non-physiological compatibility peritoneal dialysate can increase ES protein expression and MVD, which may participate in and have effects on the course of peritoneal neoangiogensis.     

血管内皮生长因子和内皮抑素在人腹膜组织的表达及其与腹膜毛细血管新生的关系

目的 检测血管内皮生长因子（VEGF）和血管内皮抑素（ES）在人腹膜组织表达,探讨两者与腹膜血管新生之间的关系。 方法 取健康对照者、尿毒症非透析患者以及腹透患者的腹膜标本,用反转录聚合酶链反应（RT-PCR）检测VEGF和ES mRNA的表达;组织免疫组化染色检测VEGF和ES蛋白质水平的表达;CD34染色计数腹膜组织毛细血管密度（MVD）。 结果 各组腹膜均有VEGF及ES表达;健康对照组、尿毒症非透析组、腹透组VEGF mRNA的相对表达量依次为0.47±0.01、0.62±0.02、0.74±0.02。VEGF免疫组化染色阳性区平均灰度值依次为95.673±2.01、117.126±2.07、140.184±2.25。ES免疫组化染色阳性区平均灰度值依次为94.902±2.38、113.380±2.33、145.489±3.05。尿毒症非透析组、腹透组VEGF mRNA和蛋白表达水平及ES蛋白表达水平表达均高于健康对照组,且腹透组升高更为明显,差异均具有统计学意义（均P < 0.05）。3组ES在mRNA水平表达量依次为0.42±0.02、0.43±0.03、0.43±0.02,各组表达差异无统计学意义（P > 0.05）。3组腹膜MVD依次为3.05±0.45、5.98±0.47、9.62±0.49,尿毒症非透析组、腹透组均高于健康对照组,且腹透组增高更为明显,差异均具有统计学意义（均P < 0.05）。 结论 腹膜透析患者腹膜组织VEGF mRNA和蛋白表达水平升高,ES蛋白表达水平也升高,这可能在长期透析所致腹膜组织新生毛细血管形成过程中发挥一定作用。     

内皮抑素在大鼠腹膜的表达及其与腹膜血管新生的关系

目的 研究大鼠腹膜血管内皮抑素（endostatin, ES）基因及蛋白表达与腹膜血管新生的关系。 方法 32只雄性SD大鼠,按随机数字表法分为正常组、肾衰竭非透析组（非腹透组）、1.5%腹膜透析组（1.5% PD组）、4.25%腹膜透析组（4.25% PD组）,每组8只。PD组经规律PD 28 d后,取各组大鼠新鲜腹膜组织,用RT-PCR检测ES mRNA表达;用组织免疫组化染色检测ES蛋白表达;以CD34染色观察腹膜组织毛细血管密度（MVD）。 结果 各组均有ES mRNA表达,正常组为0.47±0.05;非腹透组为0.45±0.04;1.5% PD组为0.46±0.04;4.25% PD组为0.47±0.03;各组表达差异无统计学意义。正常组ES蛋白表达积分0分;非腹透组积分2分;1.5%PD组积分4分;4.25%PD组呈高表达,积分9分。正常组MVD为3.13±1.13;非腹透组为5.13±1.14;1.5%PD组为9.00±1.51;4.25%PD组为10.75±1.83;组间差异均有统计学意义（P < 0.05）。 结论 尿毒症状态和非生理性腹透液刺激可使大鼠腹膜组织ES蛋白表达升高,其在长期透析所致腹膜组织毛细血管生成增多中可能发挥一定的作用。     

内皮抑素在大鼠腹膜的表达及其与腹膜血管新生的关系

Objective To investigate the expression of endostatin (ES) in rat peritoneum and its association with peritoneal neoangiogensis. Methods Thirty-two male SD rats were randomly divided into 4 groups: normal control rats (C group), renal failure without PD rats (non-PD group), rats dialyzed with 1.5% PD solution (1.5% PD group) and 4.25% PD solution (4.25% PD group). After regular PD for 28 days, mRNA and protein expression of ES in peritoneal tissues of each group were detected by RT-PCR and immunohistochemistry respectively. Microvessel density (MVD) of peritoneal tissue was assessed using immunohistochemistry with CD34 monoclonal antibody. Results ES mRNA was expressed in each group, 0.47±0.05 in C group, 0.45±0.04 in non-PD group, 0.46±0.04 in 1.5%PD group, 0.47±0.03 in 4.25%PD group, and no significant differences were found among groups. Score of ES protein expression was O in C group, 2 in non-PD group, 4 in 1.5%PD group, and 9 in 4.25%PD group. MVD was 3.13±1.13 in C group, 5.13±1.14 in non-PD group, 9.00±1.51 in 1.5%PD group, 10.75±1.83 in 4.25%PD group, and significant differences were found among groups. Conclusion Uremia circumstance and non-physiological compatibility peritoneal dialysate can increase ES protein expression and MVD, which may participate in and have effects on the course of peritoneal neoangiogensis.     

内皮抑素在大鼠腹膜的表达及其与腹膜血管新生的关系

Objective To investigate the expression of endostatin (ES) in rat peritoneum and its association with peritoneal neoangiogensis. Methods Thirty-two male SD rats were randomly divided into 4 groups: normal control rats (C group), renal failure without PD rats (non-PD group), rats dialyzed with 1.5% PD solution (1.5% PD group) and 4.25% PD solution (4.25% PD group). After regular PD for 28 days, mRNA and protein expression of ES in peritoneal tissues of each group were detected by RT-PCR and immunohistochemistry respectively. Microvessel density (MVD) of peritoneal tissue was assessed using immunohistochemistry with CD34 monoclonal antibody. Results ES mRNA was expressed in each group, 0.47±0.05 in C group, 0.45±0.04 in non-PD group, 0.46±0.04 in 1.5%PD group, 0.47±0.03 in 4.25%PD group, and no significant differences were found among groups. Score of ES protein expression was O in C group, 2 in non-PD group, 4 in 1.5%PD group, and 9 in 4.25%PD group. MVD was 3.13±1.13 in C group, 5.13±1.14 in non-PD group, 9.00±1.51 in 1.5%PD group, 10.75±1.83 in 4.25%PD group, and significant differences were found among groups. Conclusion Uremia circumstance and non-physiological compatibility peritoneal dialysate can increase ES protein expression and MVD, which may participate in and have effects on the course of peritoneal neoangiogensis.     

低氧诱导因子1α在白蛋白过负荷致肾小管上皮细胞转分化中的作用

长期蛋白尿可致肾脏纤维化,细胞外基质(ECM)的主要来源之一是转分化的肾小管上皮细胞(TEC)[1],因此上皮细胞-间充质细胞转分化(EMT)是蛋白尿患者肾脏纤维化的主要机制之一。低氧诱导因子1(HIF-1)可诱导多种细胞转分化[2]。本研究检测大鼠TEC(NRK-52E细胞)在含高浓度牛血清白蛋白(BSA)的培养基中是否发生转分化,以及shRNA- HIF -1α能否抑制BSA致NRK-52E细胞转分化的作用,以探讨HIF- 1α在蛋白尿致肾脏纤维化中的作用。     

对比剂导致急性肾损伤的预防策略

近半个世纪以来,对比剂(contrast media,CM)的研发应用使得介入放射学的广泛发展成为可能。期间对比剂几经改良,以减少其相关的不良并发症。但由于个体差异、药物代谢特性及认识不足等多种因素限制,并发症的发生仍不能完全避免,如肾毒性,已跃居医源性急性肾衰竭第3位病因[1],并     

维持性血液透析患者血清降钙素原表达水平及对感染的诊断价值

目的 探讨维持性血液透析(maintenance hemodialysis,MHD)患者血清降钙素原(procalcitonin,PCT)的水平及影响因素,评估PCT对MHD患者感染的诊断价值。方法 采用横断面研究,选取非感染的MHD患者500例,根据PCT水平将无感染患者进一步分层,采用组间比较、单因素及多因素Logistic回归法探讨MHD患者PCT升高的影响因素。另选取合并临床感染的MHD患者115例,采用受试者工作特征曲线(ROC)及曲线下面积(AUC)分析,明确PCT诊断MHD患者合并感染的最佳界值。结果 与非感染组相比,感染组MHD患者血清PCT明显升高(Z=-14.204;P<0.001)。Logistic回归分析显示：以导管为血管通路(OR=2.142,95%CI:1.282～3.579,P=0.004)、男性(OR=1.517,95%CI:1.036～2.221,P=0.032)、血红蛋白<110g/L(OR=1.792,95%CI:1.225～2.623,P=0.003)、血磷≥1.78mmol/L(OR=1.608,95%CI:1.079～2.379,...     

透析导管相关血流感染的危险因素及风险预测模型

目的:探讨留置带隧道和涤纶套的透析导管（tunnel-cuffed catheter,TCC）的血液透析（hemodialysis,HD）患者发生导管相关血流感染（catheter-related bloodstream infection,CRBSI）的危险因素,构建感染风险预测模型。方法:本研究为回顾性研究,选择2...