长沙市928名儿童微量元素调查分析

目的了解长沙市儿童血液中微量元素的含量及其相关因素,为有关部门制定卫生规划提供依据。方法收集2005年6月-2006年6月在本院检查的928例0~10岁儿童血样,用原子吸收光谱分析仪检测Ca、Mg、Fe、Zn、Cu和Pb元素的含量。结果各年龄组儿童全血中Ca、Mg、Fe、Zn、Cu含量呈偏态分布,铁、锌缺乏常见,部分儿童仍有缺钙现象。另外,总体铅高率为16.9%。结论该地区儿童微量元素营养状况处在一个较低的水平,提示儿童应注意饮食营养均衡,补充必需的微量元素,加强体育锻炼,对提高体质有一定的意义。     

急性腹泻重视程度及其相关影响因素调查

目的 对急性腹泻的重视程度进行调查,明确目前大众对腹泻的重视程度及其相关影响因素.方法 对入住我院的病 人及其家属共110人进行开放式调查,调查内容包括半年内是否有急性腹泻史、处理方式;同时对首选寻求正规医疗帮助的腹泻者(RM 组)与非首选正规医疗者(NRM组)的一般资料进行比较,寻找影响腹泻者对腹泻处理方式的可能因素.结果 在受调查的人群中半年内有急性腹泻发生率为87.3%(96人),共发生急性腹泻142次,其中首选RM、NRM的分别占28.9%、71.1%.RM组及NRM组一般资料中年龄、是否居民、个人收入比例组间差异有统计学意义.结论 大众半年内急性腹泻发生率极高,而大部分未寻求正规的诊疗,其中年龄、收入是决定人们处理方式的主要因素,故应加强基础医疗的投入,提高大众对小病的正规诊治意识.     

缺血修饰白蛋白在急性冠脉综合征早期诊断中的作用

[目的]检测探讨缺血修饰白蛋白（IMA）对急性冠脉综合征（ACS）的早期诊断价值。[方法]经确诊的ACS患者114例3 h内取血测定IMA、肌酸激酶（CK）、肌酸激酶同功酶（CK-MB）、肌钙蛋白I（cTnI）和肌红蛋白（MYO）,观察其变化水平并作比较。同时取100例健康体检者抽血测定IMA作为对照组,观察其变化水平。[结果]病例组IMA水平明显高于对照组（t=-33.56,P〈0.01）,ACS胸痛病人在胸痛发作就诊3 h内抽血检测IMA阳性率达到86.0%;明显高于其他4种心肌标志物（χ^2=442.775,P〈0.01）。[结论]IMA可对急性心肌缺血做出早期诊断,在ACS的早期诊断与排除诊断中比其他四个心肌指标更为敏感。     

茶多酚通过调节Akt通路诱导胰腺癌细胞凋亡

Objective To investigate the regulation of Akt signaling pathway involved in apeptosis of Panc-I cells induced by polyphenol ( - )-epigallocatechin-3-gallate. Methods Pane-1 cells were maintained in DMEM with 10% fetal calf serum in a 95% air,5% CO2 atmosphere,then incubated in different concentrations of EGCG (6.3,12.5,25.0,50.0 μmol/L). The growth arrest effect was determined by MTT assay, and apoptosis was detected by agarose gel electrophoresis. The levels of Akt (60×103 ) and Bad (23×103) were determined by Western blot. Results EGCG time and dose dependently repressed the growth of Panc-1 cells and induced apeptosis in Panc-1 cells ( P <0.05 ). The expression of p-Akt (set473) and p-Bad (ser136) was reduced in a dose-dependent way,whereas the pan-Akt and pan-Bad were unchanged (P < 0.05). Conclusion EGCG can induce apoptosis in pancreatic cancer cells in a dose-and time-dependent fashion by attenuating the blocking effects of Akt signaling pathway on Bad proteins.     

茶多酚通过调节Akt通路诱导胰腺癌细胞凋亡

Objective To investigate the regulation of Akt signaling pathway involved in apeptosis of Panc-I cells induced by polyphenol ( - )-epigallocatechin-3-gallate. Methods Pane-1 cells were maintained in DMEM with 10% fetal calf serum in a 95% air,5% CO2 atmosphere,then incubated in different concentrations of EGCG (6.3,12.5,25.0,50.0 μmol/L). The growth arrest effect was determined by MTT assay, and apoptosis was detected by agarose gel electrophoresis. The levels of Akt (60×103 ) and Bad (23×103) were determined by Western blot. Results EGCG time and dose dependently repressed the growth of Panc-1 cells and induced apeptosis in Panc-1 cells ( P <0.05 ). The expression of p-Akt (set473) and p-Bad (ser136) was reduced in a dose-dependent way,whereas the pan-Akt and pan-Bad were unchanged (P < 0.05). Conclusion EGCG can induce apoptosis in pancreatic cancer cells in a dose-and time-dependent fashion by attenuating the blocking effects of Akt signaling pathway on Bad proteins.     

HBV相关慢加急性肝衰竭患者血清M30和CCL20水平变化及意义

目的观察HBV相关慢加急性肝衰竭(HBV-ACLF)患者血清M30、CCL20的水平及其与短期(3个月)预后的关系,分析其临床意义。方法收集2014年10月-2017年1月在湖南省人民医院感染科诊治的HBV-ACLF患者51例(HBV-ACLF组);住院或门诊随访慢性乙型肝炎(CHB)患者30例(CHB组);健康体检者30例为对照组。HBV-ACLF患者随访3个月,根据随访结果分为存活组及死亡组。采用ELISA法检测各组基线及HBV-ACLF组患者治疗2周后外周血血清M30、CCL20水平。计量资料2组间比较采用t检验,多组间比较采用方差分析。结果治疗前HBV-ACLF组、CHB组和对照组M30、CCL20水平[(591.54±262.06)、(241.58±120.33)、(114.35±52.09)U/L;(386.51±55.12)、(177.11±27.51)、(33.95±11.62)pg/ml]3组间差异有统计学意义(F值分别为22.75、51.47,P值均<0.05)。治疗2周后HBV-ACLF死亡组患者M30水平显著高于存活组[(648.17±285.55)U/L vs(426.43±217.64)U/L,t=2.37,P<0.05],HBV-ACLF死亡组CCL20水平显著低于存活组[(232.28±39.28)pg/ml vs(352.29±52.43)pg/ml,t=-2.35,P<0.05]。治疗2周时与治疗前HBV-ACLF死亡组和存活组血清M30和CCL20水平的变化率[(0.08±0.04)vs(0.27±0.12);(0.39±0.06)vs(0.11±0.02)]差异均有统计学意义(t值分别为2.49、2.54,P值均<0.05)。结论血清M30、CCL20水平在HBV-ACLF患者中显著升高,能够较为准确的反映肝脏的细胞凋亡及炎症程度。治疗过程中CCL20水平明显下降的患者病死率更高,M30水平明显下降的患者有相对较好的预后,动态观察M30、CCL20水平变化可能对预测HBV-ACLF患者的短期预后有一定价值。     

DLG1基因多态性与炎症性肠病遗传易感性研究

目的：研究DLG1基因多态性与我国汉族人群炎症性肠病（ IBD）遗传易感性的相关性。方法：本研究共纳入345例IBD患者（溃疡性结肠炎160例,克罗恩病185例）和463例健康对照者,以及4例克罗恩病患者的15名家系成员。采用聚合酶链反应～碱基序列特异性引物法对DLG1基因第九外显子chr3＿196865242位点进行基因测序和多态性分析。结果：散发CD组GA基因型频率为18．9％,与健康对照组（11．7％）相比,差异有统计学意义,P＜0．05;散发UC组GA基因型频率为12．5％,与健康对照组（11．7％）相比,无统计学差异,P＞0．05;家系组成员GA基因型频率为60％,与健康对照组（11．7％）相比,差异有统计学意义,P＜0．05。结论：DLG1基因chr3＿196865242位点被证实存在多态性,该位点多态性可能与克罗恩病的易感性相关。     

86例胃癌行全胃切除术的远期疗效研究

目的探讨分析胃癌行全胃切除术的远期疗效。方法从2015年8月-2017年8月于我院收治的胃癌患者病例中,选取86例作为研究对象,结合不同手术方式,分成腹腔镜组和开腹组,分别行腹腔镜下全胃切除术、开腹全胃切除术,各为43例,对两组患者术中、术后相关指标、术后并发症情况以及远期疗效进行对比和分析。结果腹腔镜组患者的术中出血量、术后肛门排气时间明显优于开腹组,对比差异显著(P0.05);两组患者淋巴结清扫数目差异不具备统计学意义(P0.05);在术后并发症方面,开腹组和腹腔镜组对比差异并不显著(P0.05)。结论腹腔镜全胃切除术、开腹全胃切除术,对于治疗胃癌患者具有显著的帮助,可以有效预防并发症,给予远期临床疗效一定的保障,但是腹腔镜手术,可以免去过多损伤到机体。     

对肺結核肺切除后殘腔的探討

部分肺切除后往往遺留胸膜残腔,这种残腔若在手术后短期內不能为余肺所充滿,常誘发脓胸、枝气管胸膜瘻等併发症。为了探索肺結核肺切除后发生残腔的有关因素、預防及处理残腔的有效措施,我們将自1956年4月至1962年7月,266例肺叶切除术、肺段切除术及肺叶加肺段切除术进行分析。在266例中併行     

茶多酚通过调节Akt通路诱导胰腺癌细胞凋亡

Objective To investigate the regulation of Akt signaling pathway involved in apeptosis of Panc-I cells induced by polyphenol ( - )-epigallocatechin-3-gallate. Methods Pane-1 cells were maintained in DMEM with 10% fetal calf serum in a 95% air,5% CO2 atmosphere,then incubated in different concentrations of EGCG (6.3,12.5,25.0,50.0 μmol/L). The growth arrest effect was determined by MTT assay, and apoptosis was detected by agarose gel electrophoresis. The levels of Akt (60×103 ) and Bad (23×103) were determined by Western blot. Results EGCG time and dose dependently repressed the growth of Panc-1 cells and induced apeptosis in Panc-1 cells ( P <0.05 ). The expression of p-Akt (set473) and p-Bad (ser136) was reduced in a dose-dependent way,whereas the pan-Akt and pan-Bad were unchanged (P < 0.05). Conclusion EGCG can induce apoptosis in pancreatic cancer cells in a dose-and time-dependent fashion by attenuating the blocking effects of Akt signaling pathway on Bad proteins.