收缩期高血压病患者435例临床分析

对于收缩期高血压的研究,近年来引起了广泛的重视。本文旨在针对收缩期高血压的病因、患病率、危险因素、并发症及预后等问题进行探讨,以期摸索出其规律性,进而做好对本病的防治工作。     

急性胆囊炎腹腔镜胆囊切除180例体会

急性胆囊炎由于胆囊周围炎症粘连,组织充血水肿,三角区解剖不够清晰,增加手术风险.1999年至2009年我院共行急性胆囊炎LC 180例,现将治疗体会总结如下.     

急性胆囊炎320例腹腔镜手术时机的评价与操作体会

目的　对急性胆囊炎腹腔镜手术时机进行评价 ,并总结手术操作体会。方法　将 32 0例急性胆囊炎患者分 3组施行LC。结果　早期手术组 16 4例 ,中转手术 8例 (0 .5 % ) ;限期手术组 5 1例 ,中转手术 7例 (13.7% ) ;择期手术组 10 5例 ,中转手术 5例 (0 .5 % )。平均手术时间分别为早期组4 6min、限期组 89min及择期组 35min ,平均住院时间分别为 5 .4d、13.6d及 8.7d。本组患者均痊愈出院。结论　急性胆囊炎在发病早期是行LC的理想时机 ;Calot三角的良好显露、正确处理胆囊管和胆囊动脉是LC成功的关键 ;正确对待中转手术和掌握中转手术的指征是LC的安全保证措施     

生物反应器中的力学刺激促进组织工程软骨再生

BACKGROUND: Cartilage tissue engineering has been widely used to achieve cartilage regeneration in vitro and repair cartilage defects. Tissue-engineered cartilage mainly consists of chondrocytes, cartilage scaffold and in vitro environment. OBJECTIVE: To mimic the environment of articular cartilage development in vivo, in order to increase the bionic features of tissue-engineered cartilage scaffold and effectiveness of cartilage repair. METHODS: Knee joint chondrocytes were isolated from New Zealand white rabbits, 2 months old, and expanded in vitro. The chondrocytes at passage 2 were seeded onto a scaffold of articular cartilage extracellular matrix in the concentration of 1×106/L to prepare cell-scaffold composites. Cell-scaffold composites were cultivated in an Instron bioreactor with mechanical compression (1 Hz, 3 hours per day, 10% compression) as experimental group for 7, 14, 24, 28 days or cultured statically for 1 day as control group. RESULTS AND CONCLUSION: Morphological observations demonstrated that the thickness, elastic modulus and maximum load of the composite in the experimental group were significantly higher than those in the control group, which were positively related to time (P < 0.05). Histological staining showed the proliferation of chondrocytes, formation of cartilage lacuna and synthesis of proteoglycan in the experimental group through hematoxylin-eosin staining and safranin-O staining, which were increased gradually with mechanical stimulation time. These results were consistent with the findings of proteoglycan kit. Real-time quantitative PCR revealed that mRNA expressions of collagen type I and collagen type II were significantly higher in the experimental group than the control group (P < 0.05). The experimental group showed the highest mRNA expression of collagen type I and collagen type II at 21 and 28 days of mechanical stimulation, respectively (P < 0.05). With the mechanical stimulation of bioreactor, the cell-scaffold composite can produce more extracellular matrix, such as collagen and proteoglycan, strengthen the mechanical properties to be more coincident with the in vivo environment of cartilage development, and increase the bionic features. With the progress of tissue engineering, the clinical bioregeneration of damaged cartilage will be achieved.   中国组织工程研究杂志出版内容重点：组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松;组织工程     

氟脲嘧啶治疗急性胰腺炎52例临床分析

目的：评价氟脲嘧啶治疗急性胰腺炎的疗效。方法：对52例急性胰腺炎除了采用常规治疗措施外，应用氟脲嘧啶（5－Fu)500mg静脉滴注，每日一次。结果：52例用药4－11天（平均用药7．6天），血尿淀粉酶在4－10天后降至正常。症状在用药后3－5天缓解。治愈46例（占83％），死亡6例（占11．5％）。其中有11例中转手术。结论：氟脲嘧啶治疗急性胰腺炎是通过抑制胰蛋白酶的活性，效果良好，且胰药浓芳可达血药浓度的三倍。同时，氟脲嘧啶价格低廉，适合临床推广作常规治疗药物。     

血脂康对冠心病纤维蛋白原、血粘度和胰岛素抵抗的影响

目的;观察国产血脂康在调脂治疗的同时对冠心病患者纤维蛋白原、血粘度和胰岛素抵抗的影响。方法:54例冠心病伴血清总胆固醇(TC)>5.2mmol/L或低密度脂蛋白胆固醇(LDL-C)>3.12mmol/L患者口服血脂康(0,6,2次/d),治疗8周,观察治疗前、后TC、LDL\IC、高密度脂蛋白胆固醇(HDL-C)、甘油三酯(TG)及纤维蛋白原、血粘度和胰岛素敏感性指数(ISI)变化,另选择20例正常健康者作为对照组。结果:血脂康治疗8周后,TC、LDL-C和TG水平分别下降24.6％、34.1％和17.5％,HDL-C水平升高13.4％(P均<0.05或<0.01);纤维蛋白原和不同切变率的血粘度均显著降低(P<0.05或<0.01)。血脂康治疗前胰岛素敏感指数显著低于正常对照组,而治疗后则明显升高(P<0.05)。结论;血脂康对于冠心病患者不仅能够有效调整血脂,而且还具有降低纤维蛋白原和血粘度及改善胰岛素抵抗的有益作用。     

谷氨酰胺二肽对急性胰腺炎大鼠自由基代谢的影响

目的 :探讨谷氨酰胺二肽 (Gln)对急性胰腺炎大鼠自由基代谢及抗氧化作用的影响。方法 :采用 5 %牛磺胆酸钠逆行注入胰胆管建立急性胰腺炎模型 ,并用生理盐水、Gln等试剂预处理。分别在给药后 4 ,8,16h处死动物 ,检测血浆谷胱甘肽 (GSH)、超氧化物歧化酶 (SOD)、丙二醛 (MDA)浓度 ,并观察胰腺组织病理变化。结果 :经Gln预处理后 ,各时间点动物血浆GSH、SOD浓度均增高 (P <0 .0 5 ) ,而MDA浓度则降低 (P <0 .0 5 ) ,胰腺组织病理变化差异无显著性 (P >0 .0 5 )。结论 :Gln改善急性胰腺炎自由基代谢及抗氧化作用     

北京市东城区740名中学生青春期性知识调查

为了解中学生对性知识及预防艾滋病知识的掌握程度 ,以便更好地在学校中开展青春期健康教育、预防艾滋病的基础知识教育 ,提高青少年的健康水平 ,本文作者于 2 0 0 0年 2月对东城区两所中学的 740名学生进行了问卷调查。1　对象和方法1.1　对象　以东城区两所中学 (普通校、重点校各一所 )初一、初二、高一、高二全体学生为调查对象。共调查 742人 ,收回有效问卷 740张 ,占被调查人数的 99.73%。其中 ,男生为 36 3人 ,占 49.0 5 % ;女生 377人 ,占 5 0 .95 % ;初中生 378人 ,占 5 1.0 8% ;高中生 36 2人 ,占 48.92 %。1.2　方法　调查表由北…