重视手术后期特殊体位改变的防范意识

手术后期特殊体位改变，是指手术过程中患者需要在特殊的体位中如截石位、俯卧位、左右侧卧位等体位中完成手术，以利于手术视野的暴露和手术的顺利完成，手术结束时再将患者改变成仰卧位的过程。此时手术将成，但患者的承受力处于最低点，由于经受麻醉、手术、出血等因素刺激后，患者处于代偿与失代偿状态的边缘，幅度较大的体位改变，有可能导致一切平顺现象的破坏，出现血流动力学的急骤改变，引起一系列如血压下降、恶心呕吐、心率紊乱、大汗淋漓、面色苍白、表情淡漠等现象，更严重的导致出血而再次手术。     

Chronic Kidney Isograft and Allograft Rejection

In this study antigen-independent factor in the pathogenesis of chronic rejection of organ transplants was examined.Kidney isografts and allografts were transplanted orthotopically into bilaterally nephroectomized rat recipients and studied functionaaly,morphologically and immunohistologically,at serial intervals up to 52 weeks after transplantation.Allograft recipients developed progressive proteinuria after 12 weeks,with gradual renal failure ultimately leading to death.At the same time,morphological changes,including progressive arteriosclerosis and glomerulosclerosis ,tubular atrophy and interstitial fibrosis,developed.Immunohistologically,macrophages infiltrated glomeruli during this period and cytokines became unregulated.Our results showed that antigen-independent functional and morphological changes occurred in long-tern kidney isografts and mimicked those appearing much earlier in allografts that reject chronically.Initial injury and extent of functioning renal mass is suggested to be important factor for such late changes.     

EGSB反应器处理高浓硫酸盐废水

研究在负荷为20 kg COD/(m3.d)正常运行的EGSB(Expanded Granular Sludge Bed)反应器中,处理高浓硫酸盐废水。经过一个月左右的驯化,在进水COD为4 000 mg/L的条件下,进水SO42-质量浓度可提升至约1 800 mg/L,获得了9 kg SO42-/(m3.d)的运行能力。较高的有机负荷使得产气量较大随之带来明显的气提效应,再加上较高的上升流速所产生的良好的气固分离效果,使得气相中的硫元素含量较高,达到了质量分数43.8%。硫酸盐还原菌所能达到的最大电子流比重为31.4%,对应的最低COD/SO42-值约为2.0。EGSB反应器内溶解性硫化物与相应的自由硫化氢质量浓度为255 mg/L和102 mg/L,未对SRB与产甲烷菌产生任何毒性。     

噬菌体治疗实验性小鼠耐亚胺培南铜绿假单胞菌感染的研究

目的探讨噬菌体治疗耐药性铜绿假单胞菌感染的疗效.方法以BALB/c小鼠为实验动物,建立耐药性铜绿假单胞菌全身感染模型,应用体外试验所筛选的宽噬噬菌体进行治疗.结果噬菌体在感染复数（multiple of infection,MOI）≥0.01时能有效杀灭铜绿假单胞菌,显著提高小鼠生存率.延迟治疗3 h仍有40%的生存率.结论通过对小鼠生存率、噬菌体在体内分布及机体对噬菌体的免疫反应等指标的观察分析,噬菌体制剂简捷高效,对机体正常组织无毒副作用,从而为临床治疗全身或局部铜绿假单胞菌感染提供了新途径.     

大鼠肾移植慢性排斥反应期间白细胞介素—2受体和细胞间粘 …

为探讨移植肾白细胞介素－２受体（ＩＬ－２Ｒ）和细胞粘附分子－１（ＩＣＡＭ－１）的 肾移植慢性排斥反应中的地位及诊断价值,选用近交大鼠Ｆ３４４和ＬＥＷ进行左侧原位肾移植,于手术后１０天切除右侧自体肾分别于术后第４、１２～、６,〉３２周处死各组动物,取移植肾标本进行病理检查,并应用免疫组织化学技术ＳＰ法检测移植肾中ＩＬ－２Ｒ和ＬＣＡＭ０１的表达,结果发现同种异体肾移植组于条４周开始出现ＩＬ２Ｒ及ＩＣＡ     

3D FLAIR增强扫描在内耳内淋巴成像中的应用探讨

目的:探讨分析3D FLAIR增强扫描在内耳内淋巴成像中的应用效果。方法:2017年6月-2019年10月,本院收治行内耳内淋巴成像患者25例、健康体检者12例,对患者实施3D FLAIR增强扫描与耳蜗电图检查,对比检测结果。结果:四组的内淋巴积水程度对比存在明显差异(P<0.05)。3D FLAIR增强扫描判断内淋巴积水阳性率与耳蜗电图判断内淋巴积水阳性率存在明显差异(P<0.05)。结论:3D FLAIR增强扫描在内耳内淋巴成像中应用,可辅助临床更快、更准确的检出内耳内淋巴积水,值得在临床推广应用。     

神经内科住院病人常见护理安全隐患及防范措施

从护理安全管理出发,针对神经内科住院病人特点,提出神经内科住院病人常见护理安全隐患,制定相关防范措施,最大限度地消除不安全因素,减少护理不良事件的发生,确保护理工作优质高效地顺利进行。     

痔上粘膜环切术治疗重度痔55例临床观察

目的研究吻合器痔上粘膜环切术（PPH）治疗重度痔的可行性及其意义。方法对55例重度痔行痔上粘膜环切术，并与55例行传统手术治疗的患者进行对比分析。结果PPH方法简单、手术时间短、出血少、住院时间短，随访1～10个月，术后并发症少，无复发。结论PPH应作为重度痔的首选手术方法。     

高表达蛋白hSav1对人胚肾HEK293增殖能力的影响

Objective To elucidate the effects of human Salvador 1 (hSav1 ) on cell proliferation of human embryonic kidney cell line HEK293. Methods The plasmid CFP-N1-hSav1 was constructed and transfected into HEK293 cells with lipofectamine 2000. The transfection efficiency was detected by fluorescent microscopy. The effects of hSav1 on cell proliferation were measured by MTT and BrdU incorporation. Results The transfection efficiency was about 70% -80%. The MTT results showed that the inhibition rate of cell proliferation in transfected group at the 12th, 24th, 36th, and 48th h was 2% , 5% , 15% , and 23% , respectively;while that in the control group was 2% , 3% , 2% , and 2% respectively. The BrdU incorporation revealed that the BrdU incorporation rate in transfected group (12. 9 ±5. 3)% was significantly lower than in control group (27.3±3.8)% (P<0.05). Conclusion hSav1 is a newly identified protein that can cause cell proliferation inhibition in HEK293 cells.