诱导型一氧化氮合酶和缺氧诱导因子-1α与尖锐湿疣发生、发展的相关性研究

目的检测尖锐湿疣（CA）组织中诱导型一氧化氮合酶（iNOS）与缺氧诱导因子-1α（HIF-1α）的表达,以明确其在CA 发生、发展中的临床意义。方法收集50 例CA和20 例正常包皮组织,采用免疫组织化学法检测组织中iNOS 与HIF-1α的表达并与CD34 表达进行相关分析。结果50 例CA 患者中,iNOS 阳性50 例（100%）,表现为表皮全层细胞均为iNOS 阳性表达;对照组20 例正常皮肤中iNOS 阳性14 例（70%）,iNOS阳性表达主要位于表皮基底层,且呈弱阳性表达,CA 患者皮损中iNOS 灰度值为（97.985±20.320）高于对照组（73.017±15.633）（P <0.05）。50 例CA 患者中42 例HIF-1α阳性（84%）,高于对照组阳性9 例（45%）（P <0.05）;且HIF-1α在CA患者中灰度值为（0.204±0.064）,高于对照组（0.135±0.019）（P <0.05）。CA患者组织中iNOS 与CD34 表达呈正相关（r =0.375,p =0.007）;CA 患者组织中HIF-1α与CD34 表达呈正相关（r =0.393,p=0.005）。结论iNOS 与HIF-1α 可能协同参与CA 的发病和发展。     

妊娠并发结节性红斑1例

1资料患者阿力米热.***,女,25岁,主因双下肢反复红斑结节伴疼痛1+月来我院门诊就诊。患者1+月前无明显诱因,双小腿胫前出现鲜红色红斑,表面皮肤逐渐发生红色隆起,皮下可触及数个大小不等的小结节,并伴有疼痛,数周后原有少数红斑结节自行消退,他处再发红斑。近1+月来双侧小腿反复出现类似皮疹,后逐渐增多,就诊于外院,因妊娠3+月患者拒绝用药治疗。患者因疼痛明显,于2012年11月5日就诊于我科门诊,门诊以结节性红斑收住入院。患者孕1产0,宫内妊娠3+     

中药内服外用法治疗结节性红斑体会

结节性红斑,中医称之为"瓜藤缠",是一种发生于小腿伸侧的红色或暗红色痛性结节,以皮肤血管炎和脂膜炎为病理基础的皮肤病,其临床特征为散在的皮下结节,高于皮面,皮疹鲜红或暗红,按之疼痛,常反复发作,经年不愈。病因及发病机制尚未完全明了,现代医学将之归为结缔组织性疾病,认为     

红斑型天疱疮误诊寻常型银屑病1例

1临床资料患者女,47岁。全身红斑、糜烂伴瘙痒半年,加重3个月。半年前无明显诱因全身出现散在少量红斑,并很快形成糜烂,自诉无水疱,感瘙痒。患者未予诊治。3个月前无明显诱因全身出现较多红斑、糜烂,部分皮损上附鳞屑,感瘙痒明显,遂就诊于当地医院,诊断为"寻常型银屑病",予地奈德乳膏、卡泊三醇乳膏外擦等治疗后病情稍好转。患者为求更好疗效,遂来本院就诊。系统检查未见异常。     

丙种球蛋白在儿童重症系统性红斑狼疮治疗中的应用

目的探讨静脉注射人血丙种球蛋白治疗儿童重症系统性红斑狼疮的疗效,为该病提供新的治疗选择。方法选取2014年7月‐2016年7月该院确诊为重症系统性红斑狼疮且接受足量糖皮质激素治疗1周疗效欠佳的住院患儿42例,随机分为对照组与观察组,对照组给予泼尼松及环磷酰胺治疗,观察组给予泼尼松和人血丙种球蛋白治疗。结果两组的系统性红斑狼疮活动指数评分下降幅度不同,差异具有统计学意义,观察组的临床疗效优于对照组,且毒副作用小。结论人血丙种球蛋白为治疗儿童重症系统性红斑狼疮有效的辅助治疗措施,安全,毒副作用小,值得在临床推广。     

Gabapentin effectively alleviates pain sensitization and regulates CACNA2D1 expression in postherpetic neuralgia rats北大核心CSCD

Aim To identify the molecular target of gabapentin in the treatment of postherpetic neuralgia(PHN). Methods The molecular target of gabapentin for PHN was analyzed by network pharmacology and molecular docking and confirmed by coprecipitation test. Rats were randomly divided into control group, model group, model+50 mg·kg-1 gabapentin group, model+100 mg·kg-1 gabapentin group, and model+200 mg·kg-1 gabapentin group, with nine rats in each group. The pain-related behaviors of the rats were measured at different time points. The mRNA and protein expressions of CACNA2D1, Bax, and Bcl-2 in rat spinal cord were determined by immunofluorescence, Western blot, and qPCR. Results CACNA2D1 was the target gene of gabapentin that determined via network pharmacology, molecular docking, and co-precipitation tests. After modeling, mechanical pain threshold and thermal pain threshold significantly decreased, and the number of apoptotic GABA cells significantly increased. However, after intraperitoneal injection of 50, 100, and 200 mg·kg-1 gabapentin, mechanical pain threshold and thermal pain threshold significantly increased(P<0.05), and the number of apoptotic GABA cells significantly decreased(P<0.01). Immunofluorescence and Western blot results showed that compared with the model group, with the increase of gabapentin concentration, the positive expression rate of Bax significantly decreased, and the positive expression rate of Bcl-2 and CACNA2D1 significantly increased. The mRNA expression levels of Bax, Bcl-2 and CACNA2D1 detected by qPCR were consistent with the results of immunofluorescence and Western blot. Conclusions Gabapentin up-regulates the expression of target protein CACNA2D1, inhibits the proapoptotic protein Bax, and promotes the expression of apoptotic inhibitor Bcl-2. © 2023 Publication Centre of Anhui Medical University. All rights reserved.     

Curry-Jones综合征1例国内首报

患儿男, 3岁6个月, 生后即发现多发带状分布的白色斑疹, 随身体等比例生长, 无自觉症状, 伴右手拇指畸形及右手活动障碍。患儿平素不喜与人交流, 注意力较同龄儿童差。否认家族史。体检:神志清, 精神可, 语言表达欠佳;特殊面容, 右手拇指关节较左侧略膨大;心、肺、腹未见异常。皮肤科检查:右胸部、右上肢屈侧、下腹正中线及右下肢可见大小不等的多发片状、带状不规则白色斑疹, 部分斑疹融合, 大致沿Blaschko线分布, 局部略隆起;右手掌、拇指桡侧可见条带状褐色斑。下肢白斑皮损组织病理检查示基底样毛囊错构瘤。头颅磁共振成像提示胼胝体发育不全。患儿病变组织全外显子测序显示SMO基因突变c.1234C>T(p.L412F), 父母均无该位点突变。根据皮损表现、病理及基因检测结果, 该患儿诊断为Curry-Jones综合征, SMO基因突变c.1234C>T(p.L412F)可能是其致病原因。予患儿右手拇指功能锻炼并密切随访。