γH2AX和53BP1蛋白在肺正常上皮细胞DNA氧化损伤反应中的表达

目的探讨肺正常上皮细胞(human bronchial epithelial cells,HBE)中磷酸化组蛋白H2AX(phosphorylated H2AX,γH2AX)和p53结合蛋白1(p53-Binding protein 1,53BP1)在DNA氧化损伤反应中的表达。方法用0、25、50、100、200、400μmol/L过氧化氢(H2O2)处理HBE细胞1 h,对其造成氧化损伤引起DNA双链断裂(double strand breaks,DSBs);CCK-8比色法检测H2O2对HBE细胞活性的影响,流式细胞术检测细胞凋亡情况,荧光显微镜检测HBE细胞核内γH2AX和53BP1蛋白的聚集情况,western blot检测γH2AX、53BP1、BRCA1蛋白的表达水平。结果与对照组比较,25μmol/L H2O2处理组细胞活性(1.07±0.01)升高,50、100、200、400μmol/L H2O2处理组细胞活性(分别为0.97±0.01,0.96±0.01,0.95±0.01和0.94±0.01)降低,差异具有统计学意义(F=50.35,P<0.01)。细胞凋亡检测结果显示,与对照组[(4.65±0.32)%]比较,50、100、200、400μmol/L H2O2处理组细胞凋亡率[分别为(7.54±0.57)%、(7.84±0.68)%、(8.40±0.50)%和(14.03±1.03)%]升高(F=35.879,P<0.01)。与对照组比较,经25、50、100、200、400μmol/L H2O2处理后γH2AX的平均荧光强度升高(F=223.97,P<0.01),而经50、100、200、400μmol/L H2O2处理后53BP1的平均荧光强度降低(F=117.78,P<0.01);western blot结果显示,随着H2O2浓度升高,γH2AX蛋白表达水平升高,BRCA1和53BP1的表达水平下降,差异均具有统计学意义(F分别为96.20,11.55,21.92,P均<0.01)。结论在H2O2导致HBE细胞的氧化损伤中,γH2AX可作为DNA氧化损伤标志物,但53BP1的表达下降提示HBE细胞可能通过其他途径进行DNA氧化损伤的修复。     

Renal response and its predictive factors of lupus nephritis: a 2-year real-world study of 56 hospital-based patients

Clinical Rheumatology - Our aim was to evaluate the renal response rate of patients with lupus nephritis (LN) undergoing standard treatment during a 2-year follow-up and to investigate its...     

Surgical management of Squamous Cell Carcinoma of the lower lip: An experience of 109 cases

Objectives: We are presenting our experience collected from a series of 109 cases with SCC of the lower lip focusing on clinical features of patients and surgical approach. Study Design: We retrospectively analyzed medical records of patients diagnosed with Squamous Cell Carcinoma (SCC) of the lower lip at the Oral and Maxillofacial surgery at Xi’an Jiaotong University during a period between 1999 and 2008. Results: A total of 109 patients with lip cancer were included in the study. When no frozen-section test was performed, the neoplasia was removed with a margin of at least 6 mm. Different surgical techniques were used for lip reconstruction after tumor excision. Neck dissection was performed in all patients with clinically palpable lymph nodes. Median follow-up was 38 months. During follow-up, recurrence occurred in 5 patients, 3 patients developed neck metastases, distant metastases developed in 1 patient. Five patients died during observation period. Conclusions: The patient-related and defect-related issues must be taken into consideration during reconstruction for surgical defect. For N0 patients, we recommend wait-and-see policy. Early detection, careful follow-up and prompt neck is essential for the successful treatment. Key words:Lip cancer, surgical management, reconstruction.     

一种快速检测尿中微量白蛋白的高效液相色谱法

目的:建立一种快速检测尿中微量白蛋白的高效液相色谱检测方法。方法:采用新的流动相建立分子筛-高效液相色谱法测定人血清白蛋白标准液和尿液白蛋白, 对该法进行方法学性能研究并分析56 例糖尿病患者的尿液标本。结果:色谱柱Agilent Zorbax GF-250 以0.1% 甲酸水溶液与乙腈(85:15) 为流动相, 流速1 mL/min, 检测波长205nm 时, 人血清白蛋白标准液和尿液白蛋白均在1.7 min 出峰。在5~2000 mg/L 范围内线性关系良好(r²=0.9969);检测低限为2 mg/L;批内和批间变异系数分别为3.98% 和4.05% (20 mg/L)、3.55% 和3.60%(200 mg/L) 及4.65% 和4.74% (2000mg/L);回收率分别为95.3%, 98.1% 和97.2%。56 例糖尿病患者标本, 高效液相色谱法能检出30 例微量白蛋白尿, 免疫散射比浊法检出15 例, 高效液相色谱法的检出率明显高于免疫散射比浊法(P<0.05)。结论:采用甲酸水溶液与乙腈为流动相的分子筛- 高效液相色谱法能较其他方法检出更多的微量白蛋白, 且快速, 灵敏度高, 适合临床常规测定。     

乙型肝炎患者血清IL-1β、IL-6和IL-8水平检测及意义

目的　研究血清白细胞介素 - 1β(Interleukin- 1β,IL - 1β)、白细胞介素 - 6 (IL - 6 )和白细胞介素 - 8(IL -8)水平与慢性乙型肝炎 (CHB)的关系。　方法　采用放射免疫分析法 (RIA)检测了 96例 CHB患者及 4 8例健康人血清IL - 1β、IL - 6及 IL - 8的浓度。　结果　 CHB患者血清 IL - 1β、IL - 6和 IL - 8水平均显著高于正常对照组 (P<0 .0 1)CHB患者 IL - 1β、IL - 6和 IL - 8水平增高与血清 HBV- DNA呈正相关 (P<0 .0 1)。　结论　 IL - 1β、IL - 6和 IL - 8参与 CHB免疫病理损伤过程 ,检测血清 IL - 1β、IL - 6和 IL - 8在判断 CHB患者肝损伤程度及预后有临床实用价值     

CBCT-based bone quality assessment in decompression of large odontogenic cystic lesions

Objectives

Bone quality comprises bone mineral density and trabecular microstructure. The aim of this study was to explore the effectiveness of cone-beam computed tomography (CBCT) in evaluating bone quality of large odontogenic cystic lesions after decompression using CBCT and BoneJ software, and to determine whether secondary definitive surgery can be guided using CBCT data.

Methods

Twenty-seven patients with large odontogenic cystic lesions treated by decompression were evaluated by CBCT. Medical history and perioperative details were analyzed.

Results

The \(\Delta\)CT values for all patients with cystic lesions decreased after decompression, with no differences for age, sex, and histology (p?>?0.05). Bone volume fraction and trabecular number of new cancellous bone (0.012%, 0.17/mm³) were lower than those of normal cancellous bone (0.189%, 0.47/mm³) (p?<?0.05), while new cancellous bone trabecular separation (11.344?±?2.556 mm) was stronger than normal cancellous bone trabecular separation (4.833?±?2.232 mm) (p?<?0.05). There were no differences in trabecular thickness between new cancellous bone (3.812?±?1.593 mm) and normal cancellous bone (4.598?±?3.573 mm) (p?=?0.746). The \(\Delta\)CT values of five patients with favorable osteogenesis were ??72, ?86, ??86, ?47, and ??55, those of three patients with moderate osteogenesis were ??107, ?120, and ??71, and those of two patients with poor osteogenesis were ??165 and ??127 during secondary definitive surgery.

Conclusions

CBCT is considered beneficial for evaluating bone quality of large odontogenic cystic lesions after decompression, while providing potentially useful information for referral to secondary definitive surgery.

     

高能量Pilon骨折的分期治疗策略

目的：探讨分步延期内固定方法治疗高能量Pilon骨折的临床疗效。方法：2006—03—2012—03收治高能量Pilon骨折31例,其中开放性骨折26例,闭合性骨折5例;Reudi—Allgower分型,Ⅱ型13例,Ⅲ型18例。人院后根据软组织损伤情况及开放和闭合性骨折分类,均行急诊手术,闭合伤口,开放性骨折组先行清创术,一期行腓骨骨折切开复位钢板固定,胫骨骨折应用超关节外固定架临时固定或跟骨牵引;待肿胀消退、软组织条件恢复后,二期拆除外固定行胫骨远端骨折内固定治疗。结果：31例骨折全部愈合,愈合时间12～24周,平均13周。术后所有患者均获随访,随访7～58个月,平均随访35个月。按Burwell Chamley Pilon骨折复位放射学评价标准：解剖复位22例、复位可6例、差3例,优良率为90．32％。按Tornetta骨折临床疗效评价术后功能：优18例、良7例、可4例、差2例,优良率为80．65％。术后无骨不连、骨髓炎、深部软组织感染病例,发生创伤性关节炎2例,骨折延迟愈合1例。结论：高能量Pilon骨折的分步延期治疗策略兼顾了软组织损伤及骨折碎裂重的特点,充分发挥了外固定及内固定两者各自的优势,较大地减少了手术并发症,取得了较好的临床效果,是一个较安全有效的治疗方案。     

Face-lift incision combined with sternomastoid muscular flap in parotidectomy

Background  

To improve the aesthetic outcome of a traditional parotidectomy, this study used a face-lift incision combined with a sternomastoid muscular (SCM) flap and compared the classical method with these techniques from the patient’s perspective.     

光固化玻璃离子水门汀和复合树脂联合修复楔状缺损的临床研究

目的：评价玻璃离子水门汀和复合树脂联合修复楔状缺损的临床疗效。方法：对90例540个楔状缺损牙分别用玻璃离子水门汀、复合树脂和二者联合应用修复，观察1—2年的修复效果。结果：玻璃离子水门汀修复楔状缺损2年成功率高于复合树脂修复(P＜0．05)，二者联合应用1年及2年成功率均高于二者单独应用的成功率(P＜0．05)。结论：玻璃离子水门汀和复合树脂联合应用是修复楔状缺损较好的方法。