Distribution of Hepatitis B Virus Genotypes and Its Clinical Significance in Hubei Province, China

The distribution of hepatitis B virus genotype in Hubei province and its clinical signifi- cance were investigated. HBV genotypes of 276 patients were detected by PCR-microplate sandwich hybrization-ELISA technique. The level of HBV DNA was detected by using PCR-fluorescence quantification test. Among 276 patients, there were 78 cases of HBV asymptomatic carriers, 110 cases of chronic hepatitis B (CHB), 62 cases of severe hepatitis (SH) or liver cirrhosis (LC) and 26 cases of hepatocellular carcinoma (HCC). The genotypes of HBV included C, B, mixtures (B C, B D, C D) and D, accounting for 55.8%, 25.4%, 16.7% and 2.1% respectively. The average level of HBV DNA in genotypes C, B, mixtures and D was 1.20×106, 7.81×104, 3.26×105 and 5.01×104 cop- ies/mL respectively. The ratio of SH, LC and HCC in genotype B, C and mixtures was 20%, 30% and 48% respectively. Statistical analysis revealed the percentage of genotype mixtures infection was sig- nificantly higher than that of genotype B infection. There was no significant difference in the per- centage between genotype B and genotype C or between genotype C and mixtures. The distribution of genotype B, C and mixtures in SH, LC and HCC was significantly different. The frequency of HCC was zero in patients with co-infection. Genotype D was only related with SH and LC. The in- creased ALT could be converted to categorical grades of severity. From mild, moderate to severity, the prevalence of genotype C showed an opposite trend, although no statistically significant differ- ence was observed. The HBeAg positive rate was higher in patients with genotype C infection than in those with genotype B, especially in the patients whose ages were from 31 to 40 years old. Compared with genotype B, genotype C showed a higher HBeAg positive rate in patients with SH and LC. The percentage of SH, LC and HCC was higher in patients with genotype C and mixtures infection. On the contrary, the percentage of genotype B was lower. The HBeAg positive rate in patients with genotype C infection was higher than those with genotype B infection. Genotype C and mixtures may be associated with development of severe liver disease.     

血吸虫病患者吡喹酮治疗后外周血T细胞亚群的检测

应用碱性磷酸酶抗碱性磷酸酶桥联酶标法对１８例慢性血吸虫病患者，２２例晚期血吸虫病患者进行了吡喹酮治疗前后的外周血Ｔ细胞亚群检测，发现治疗前慢血，晚血ＣＤ＾＋３，ＣＤ＾＝８百分比均显著升高，与对照组比较有显著性差异。ＣＤ＾＋４百分比低于对照组，无显著性差异。     

血吸虫病患者吡喹酮治疗后外周血T细胞亚群的检测

应用碱性磷酸酶抗碱性磷酸酶（ＡＰＡＡＰ）桥联酶标法对１８例慢性血吸虫病（慢血）患者,２２例晚期血吸虫病（晚血）患者进行了吡喹酮治疗前后的外周血Ｔ细胞亚群检测,发现治疗前慢血、晚血ＣＤ３＋、ＣＤ８＋百分比均显著升高,与对照组比较有显著性差异。ＣＤ４＋百分比低于对照组,无显著性差异。治疗３个月后检查,两组ＣＤ４＋上升、ＣＤ８＋减少,其百分比基本达到正常水平,ＣＤ４＋／ＣＤ８＋比值上升高于对照组,有显著性差异。结果提示,吡喹酮治疗血吸虫病可能具有参与免疫调节、增强Ｔ淋巴细胞功能的作用,但不能改善免疫紊乱。     

干扰素抗性和病毒性肝炎

干扰素(IFN)对各种病毒性肝炎及相关肝病都有不同程度的疗效。但是长期使用失效及疾病复发成为IFN治疗的重大缺陷。IFN抗性具有多种机制,本文就IFN抗体的产生、Ⅰ型IFN受体表达的下降、病毒蛋白的干扰和前炎性因子的表达显著增高4个方面进行综述。     

Study on the Correlation of Plasma NO, ET-1 and ALT in the Patients with Chronic Hepatitis and Cirrhosis

Nitric oxide ( NO) is a small molecular sub-stance with many biological functions.NO is an un-stable molecule with free radical( or group) struc-ture. Its half life in vivo is 1 to 5s and is reduced tothe stable terminal products( NO2 -/NO3 -) quickly.Atpresent,many researchers reported that the levelof NO is related with the degree of liver disease. Wedetermined the levels of NO,endothelin- 1 ( ET- 1 )and ALT in the in- patients with chronic hepatitis Band active cirrhosisand analyze t…     

茵栀黄特殊不良反应1例报告

茵栀黄注射液系中药有效成分提取物制成的注射液,其主要成分包括黄芩甙、茵陈、栀子,因其有清热、解毒。利湿等功能,目前在临床上广泛用于急、慢性肝炎和重型肝炎,作为退黄的主要药品之一。由于个体差异及药品纯度等原因,临床上少数患者(约3％以下)在使用中有皮疹等过敏反应,也有极少数病人出现输液反应,上述不良反应经停药及抗过敏治疗后均可缓解。最近我们在治疗肝硬变病人过程中,出现1例特殊不良反应者,现报道如下。 患者男性,45岁,临床诊断:慢性乙型病毒性肝炎(重度)、肝炎肝硬变,既往无心血管疾病病史,治疗在降酶、退黄、对症等综合治疗基础上,同时应用茵栀黄,1周内患者未出现任何不良反应,1周后在输茵栀黄过程中,患者逐渐出现胸骨     

《国际流行病学传染病学杂志》稿约

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人粒细胞无形体病研究进展

人类粒细胞无形体病（human granulocytic anaplasmosis.HGA）是一类新发的重要的人兽共患自然疫源性疾病,由嗜吞噬细胞无形体（anaplasma phagocytophilium,AP）引起,主要侵染人末梢血中性粒细胞,经蜱源传播的埃立克体疾病.     

APOBEC3G在慢性乙型肝炎、肝癌组织中的表达及与HBsAg、HBcAg的关系

目的 探讨APOBEC3G在正常对照、慢性乙肝患者肝脏组织及肝癌组织中的表达情况,以及在慢性肝炎组织中与HBsAg、HBcAg表达的关系.方法 收集正常对照肝组织9例、慢性肝炎肝脏组织37例,以及肝癌组织13例,运用免疫组织化学染色的方法检测APOBEC3G、HBsAg、HBcAg的表达.结果 与正常肝脏相比,慢性乙肝肝炎患者肝脏组织中APOBEC3G的表达增强,差异具有显著性(P<0.05),主要定位于HBV感染的肝细胞及汇管区的炎性细胞胞浆中,但与同时在肝细胞内高度表达的HBsAg、HBcAg强度无明显相关性,r分别为0.04和-0.15.P>0.05.肝癌组织中APOBEC3G也有表达,但未明显强于癌旁组织(P>0.05).结论 HBV慢性感染肝细胞中表达增高的APOBEC3G未能有效控制病毒的活动,并可能与肝癌的发生无直接关系.