用免疫化学分析仪测定正常人精液中P^30的含量

This paper is the first report about the measurement of p30 level in the normal human seminal plasma by the Beckman immunochemistry system (ICS) using anti-p30 serum. The p30 levels of 108 samples of normal human seminal plasma were measured. The range of p30 level was 0.2996-4.3913 mg/ml. The square root transformation statistical analysis indicated that the coefficient of skewness was 0.0237 the coefficient of kurtosis was -0.8854, the p30 level in normal human seminal plasma fitted the square root normal distribution, the mean was 1.6236 mg/ml and the standard deviation was 0.1641 mg/ml.     

成都汉族群体五个短串联重复序列基因频率及其种属特异性研究

目的对成都汉族群体5个短串联重复序列（short tandem repeat，STR）基因座的等位基因频率及其种属特异性进行研究，探讨其在法医学中的应用价值。方法用PCR扩增、非变性聚丙烯酰胺凝胶电泳、硝酸银染色方法，对100名成都汉族无血缘关系健康个体的5个STR基因座的等位基因频率及其种属特异性进行研究。结果D18S979、D11S2014、D18S548、D1S1667和GATA164F07在成都汉族群体中等位基因个数分别为6、5、5、7、6；基因型个数分别为12、11、13、19、14；基因型分布符合Hardy-Weinberg平衡定律。通过种属特异性分析发现5个STR基因座中，猴在D18S979、D11S2014和D1S1667基因座均检测出扩增产物，但未在人类基因座分型区内；D18S979位点人类分型区内可检测到牛、狗、鳝鱼有扩增产物，猪、鸭、鼠、兔有微弱扩增产物；D18S548位点人类分型区内只检测到牛有微弱扩增产物；D1S1667位点人类分型区内检测到狗、羊、鳝鱼有扩增产物；GATA164F07位点人类分型区内只检测到狗有微弱扩增产物；泥鳅、鸡、豚鼠在5个基因座均未检测到扩增产物。结论5个短串联重复序列中D18S979、D18S548、D1S1667和GATA164F07在成都汉族群体中具有较好的遗传多态性，D11S2014、D18S548和GATA164F07具有较好的种属特异性，在法医学个人识别和亲子鉴定中有应用价值。     

人类精浆特异性抗原P^30理化性质的初步研究

Human seminal plasma specific antigen p30, which has been characterized by other authors under the name of prostate-specific antigen or gamma-seminoprotein, has been purified. This communication describes the physico-chemical characterization of p30 demonstrated by various SDS-polyacrylamide gel electrophoresis and isoelectrofocusing as well as amino acid analysis. The results showed that the molecular weight of p30 was 33 kd and its isoelectric point was around pH 6.9. Its amino acid composition was similar to that of prostate-specific antigen. It is concluded that p30 purified by our laboratory and prostate-specific antigen named by Wang are the identical one of isomers.     

椎管动态造影在经皮穿刺髓核切吸术中的指导意义

目的：通过椎管动态造影选择经皮髓核切吸术（Automated Percutaneous Lumbar Discetomy APLD)的适应证。方法：用“Isovist”10ml腰穿注入,在X线透视下观测椎间盘突出正,侧位弧形压迹的大小,边缘光滑度及椎间隙宽度,再观察腰椎在纵向牵引下或前屈位时压迹的深浅变化,捕捉时机摄X线点片,60例全部作APLD术。结果：60例中,优46例（76．67％）,。良好3例（5％）,可2例（3．38％）,差9例（15％）,有效率达85％,椎管动态造影APLD的适应证为：（1）正位压迹≤4mm,(2)侧位压迹≤椎管矢状径1／3,（3）纵向牵引或前屈位下压迹变浅或消失;（4）病变椎间隙等宽或前窄后宽。（5）压迹弧度边缘光滑,整齐,规则。结论：椎管动态造影可以替代CT作为APLD术前常规影像学检查方法。     

正中森形成走行变异一例

在局部解剖教学的100例尸解中,发现约15岁女尸的左正中神经形成和走行发生变异。正中神经由内侧束发出的内侧根(粗约1.2mm)和外侧束发出的三个支组成;在左侧腋动脉第三段处内侧根和第1支会合;在第1支远侧15mm处第2支(0.5mm)加入后形成正中神经主干(粗3mm);在第2支起点远侧4cm处发出第3支(粗2mm)。此支和肌皮神经分开后沿肱动脉外侧走行,在旋前圆肌上缘处汇入主干。正中神经主干先位于肱动脉外侧,在下降途中经肱动脉前面逐渐转至其内侧。     

应用抗p30血清检验人类精斑的研究

The present communication describes the identification of human semen stains using immunodiffusion test with anti-p30 serum. In order to evaluate objectively the value of the immunodiffusion test for the identification. Random sampling and blind study were used. The results indicated that the immunodiffusion test was more accurate than sperm detection. It was proved that p30 was a useful human semen marker especially in semen stains of azoospermia and vaseligation. The vaginal secretion and saliva in mixed stains did not interfere with the result of the immunodiffusion. The human semen stains can be differentiated from the animal semen stains with anti-p30 serum. Therefore, it is concluded that the demonstration of p30 in a suspected stain is the best method for human semen identification up to now.