PET/CT在评价及观察大鼠肺癌放疗效果中的应用

Objective The aim of this study was to understand the role of PET/CT in monitoring the therapeutic effect of radiotherapy (RT) on lung cancer with Wistar rats. Methods Thirty Wistar in-bred strain rats (6-8 weeks, weighed 180-280 g, female, ordinary) were made into Lewis pulmonary tumor model rats. 18F-fluorodeoxyglucose (FDG) PET/CT was performed when tumor reached 1.5-2.0 cm in greatest diameter (4-6 weeks) as a baseline. In order to get the optimal time point of PET/CT for moni-toring RT effect in rat cancer model, PET/CT was performed at the 3rd day, 1st, 2nd, 3rd, and 4th week after giving single dose of 5 Gy to each rat. Standardized uptake values (SUV) from FDG PET were measured and rats were sacrificed at different time point for validation. Besides, the expressions of glucose transport1 (Glut1) in tumor tissue were studied using immunohistochemistry. The level of tumor cell apoptosis, degra-dation, and necrosis were observed. SPSS 11.0 software was used for data analyses. Results A negative correlation of SUV uptake and time after RT and negative correlation of Glut1 expression and time after RT were observed in rat tumors, respectively. Positive correlation of SUV uptake and Glut1 expression in rat tumors was observed (Spearman rank correlation test, rs = 0.97, P < 0.01). Before RT, the SUV in rat tumor was 1.28 ± 0.31 and was decrease to 1. 00 ± 0.23 at the 3rd day and 0.18 ± 0. 10 at the 4th week after RT (F=15. 126, P<0.05). Before RT, the Glut1 in rat tumor was 0.2558 ±0.03 and was decrease to 0. 2320 ± 0. 01 at the 3rd day and 0. 1320±0.04 at the 4th week after RT. The amounts of tumor cell apopto-sis, degradation, and necrosis increased with time after RT. Conclusion Though FDG PET could monitor the therapeutic effect at the 3rd day after giving single dose of RT to rat lung tumor model, the optimal time was the the 4th week after treatment.     

核素显像对消化道出血的诊断价值

目的探讨放射性核素胃肠道出血显像及异位胃黏膜显像在消化道出血诊断中的应用价值．方法诊断为消化道出血的患者85例，其中小儿62例（男性34例，女性28例，年龄22天-14岁）。成人23例（男性11例，女性12例．年龄16～63岁）。小儿 儿患者均先行99^Tc^mO4^-异位胃黏膜显像，阴性者再行99^Tc^m-RBC胃肠道出血显像；成人患者直接行胃肠道出血显像．结果62例小儿患者异位胃黏膜显像阳性率72．58％（45／62）．阳性预测值100％；胃肠道出血显像定位诊断阳性率42．5％（17／40），准确定位诊断准确率为82.35％（14／17）。结论99^Tc^mO4^-异位胃黏膜显像对小儿异位胃黏膜症诊断具有很高诊断价值．99^Tc^m-RBC胃肠道出血显像也可作为消化道出血定位诊断的一种有效方法．     

放射性核素动态显像在小儿先天性肥厚性幽门狭窄中的临床应用价值

目的:评价放射性核素动态显像在小儿先天性肥厚性幽门狭窄中的临床应用价值。方法:将对照组、病例组、手术组患儿进行放射性核素液体胃排空动态显像,对所有图像进行反流及其持续时间的观察,定量计算反流率、胃排空率和半排时间。结果:病例组反流率、反流持续时间均高于对照组,手术组反流率和反流持续时间均较病例组降低;与对照组比较,病例组胃排空率明显降低(P<0.05),半排时间延长(P<0.05);与病例组比较,手术组胃排空率升高(P<0.05),其半排时间缩短(P<0.05),而手术组与对照组比较,胃排空率和半排时间差异无显著性(P>0.05)。结论:放射性核素动态胃排空显像是动态、定量评价小儿先天性肥厚性幽门狭窄胃排空能力、反流状况及其治疗效果的一项重要的无创性检测手段。     

放射性核素肝胆动态显像对胆囊切除术后患者胆汁动力学研究

目的：应用肝胆动态显像测定胆囊切除术后患者及正常对照组的胆汁动力学变化参数,从而为诊断Oddi′s括约肌（SO）功能障碍（SOD）提供依据。方法：正常对照组12例,胆囊切除术后患者18例。所有受试者行脂餐介入肝胆动态显像,之后数据处理得出胆汁排泌参数。结果：与正常对照组比较,胆囊切除术后组十二指肠显影时间（DAT）及胆总管通过时间（HDTT）差异有显著;而肝高峰摄取时间（Tmax）、半排时间（T1/2）、胆总管高峰摄取时间（Tmax）及半排时间（T1/2）差异无显著性;脂餐介入后胆总管半排时间明显缩短。结论：胆囊切除术后患者的平均胆汁排空速度较术前速度加快,且胆汁动力学参数在不同患者间仅存在微小的变异,这些参数将为SO功能障碍患者的检出提供依据。     

异位胃粘膜显像对小儿消化道出血的诊断价值

高锝酸盐(99 mTcO4-)异位胃黏膜显像对胃粘膜异位症具有独特的诊断价值,该方法简单、无创、准确性高,已广泛应用于临床。本文对近4年来38例高锝酸盐异位胃黏膜显像阳性的Meckel’s憩室和肠重复畸形患儿的临床特征、显像结果进行分析,并与手术结果和病理诊断进行比较。1资料与方法1·1资料1999年1月~2003年8月因便血或腹痛怀疑存在异位胃黏膜症而行高锝酸盐异位胃黏膜显像呈阳性表现的患儿38例。其中男25例,女13例,年龄22 d~14岁。男女比例为1·92:1。绝大多数患儿有便血的临床表现(36/38),可为暗红色(17/38)、鲜红色(12/38),也可为黑色(7/…     

^18F-FLT、^18F-FDG PET-CT显像评价Wistar大鼠Walker256肿瘤放疗后早期疗效的研究

目的：应用PET-CT研究^18F-FLT、18F-FDG摄取在肿瘤放疗后早期疗效评价中的应用价值.方法：荷Walker 256肿瘤Wistar大鼠放疗前及放疗后24 h、48 h分别行^18F-FLT与^18F-FDG PET-CT显像,定量测定放疗前后肿瘤^18F-FLT与18F-FDG摄取（肿瘤部位ROI内放射性计数与对侧相同部位软组织相同面积ROI内放射性计数比值（T/NT）、每克肿瘤组织摄取放射性百分比（％ID/g））及肿瘤组织Ki-67阳性指数,统计分析放疗前后肿瘤摄取的变化以及与肿瘤细胞增殖活性的相关性.结果：放疗后24 h及48 h大鼠肿瘤部位18F-FLT放射性分布较对照组降低（P＜0.001）.与对照组相比,LI-Ki-67在放疗后24 h及48 h均明显降低（p＜0.001）.放疗后24 h及48 h的^18F-FDG放射性分别较对照组降低不明显（P＞0.05,P＞0.01）.放疗前后^18F-FLT及^18F-FDG摄取（T/NT）与％ID/g均呈正相关（r分别0.807和0.813）.结论：Wistar大鼠Walker 256肿瘤放疗后早期,18F-FLT摄取降低,且与肿瘤细胞增殖活性呈正相关,而18F-FDG摄取降低不明显,与肿瘤细胞增殖活性相关不明显.^18F-FLT可用于Wistar大鼠Walker 256肿瘤放疗后早期疗效的评价.     

18F-FDG PET-CT全身显像肠道高浓聚的临床意义分析

目的：评价PET-CT全身显像肠道摄取18F-FDG的临床意义.方法：本研究回顾性分析我院PET/CT中心2010年7月-2011年6月2387例检查者中肠道出现18F-FDG高代谢灶的227例检查者,通过病理结果及相关影像学检查及临床随访,确定最终结果.结果：227例肠道高浓聚中,局灶性浓聚165例,其中恶性病灶49例,总恶性率为29.70％,分组恶性率分别为体检组为8.51％,肠外原发肿瘤组为13.11％,查找原发病灶组为43.59％,临床怀疑为肠道肿瘤组为68.97％.弥漫性肠道代谢增高者62例,全部为生理性摄取或炎性改变.局灶性病灶中良性与恶性组SUV无统计学差异.结论：18F-FDG PET-CT全身显像对肠道肿瘤检出具有重要的意义,但其良恶性鉴别不能单纯依靠SUV,其病史在诊断中也具有非常重要的作用,最终诊断依靠肠镜活检.     

PET/CT在评价及观察大鼠肺癌放疗效果中的应用

Objective The aim of this study was to understand the role of PET/CT in monitoring the therapeutic effect of radiotherapy (RT) on lung cancer with Wistar rats. Methods Thirty Wistar in-bred strain rats (6-8 weeks, weighed 180-280 g, female, ordinary) were made into Lewis pulmonary tumor model rats. 18F-fluorodeoxyglucose (FDG) PET/CT was performed when tumor reached 1.5-2.0 cm in greatest diameter (4-6 weeks) as a baseline. In order to get the optimal time point of PET/CT for moni-toring RT effect in rat cancer model, PET/CT was performed at the 3rd day, 1st, 2nd, 3rd, and 4th week after giving single dose of 5 Gy to each rat. Standardized uptake values (SUV) from FDG PET were measured and rats were sacrificed at different time point for validation. Besides, the expressions of glucose transport1 (Glut1) in tumor tissue were studied using immunohistochemistry. The level of tumor cell apoptosis, degra-dation, and necrosis were observed. SPSS 11.0 software was used for data analyses. Results A negative correlation of SUV uptake and time after RT and negative correlation of Glut1 expression and time after RT were observed in rat tumors, respectively. Positive correlation of SUV uptake and Glut1 expression in rat tumors was observed (Spearman rank correlation test, rs = 0.97, P < 0.01). Before RT, the SUV in rat tumor was 1.28 ± 0.31 and was decrease to 1. 00 ± 0.23 at the 3rd day and 0.18 ± 0. 10 at the 4th week after RT (F=15. 126, P<0.05). Before RT, the Glut1 in rat tumor was 0.2558 ±0.03 and was decrease to 0. 2320 ± 0. 01 at the 3rd day and 0. 1320±0.04 at the 4th week after RT. The amounts of tumor cell apopto-sis, degradation, and necrosis increased with time after RT. Conclusion Though FDG PET could monitor the therapeutic effect at the 3rd day after giving single dose of RT to rat lung tumor model, the optimal time was the the 4th week after treatment.     

PET／CT在评价及观察大鼠肺癌放疗效果中的应用

目的观察Wistar大鼠肺癌放疗后不同时期的PET／CT表现,观察和评价大鼠肺癌放疗疗效的时间点。方法将30只近交系Wistar大鼠（鼠龄6～8周,体质量180～280g,均为雌性普通级）制作成Lewis肺癌的模型鼠,肿瘤模型建立4—6周后当肿瘤长到最大径1．5～2．0cm时,对大鼠进行^18F-脱氧葡萄糖（FDG）PET／CT显像;显像后对大鼠进行肿瘤局部单次放疗,放疗剂量为5Gy。放疗后第3天和第1、第2、第3、第4周末再对大鼠进行PET／CT显像,观察放疗的疗效,从而评价疗效观察的时间点。而后取一部分肿瘤组织制成免疫组织化学切片,观察葡萄糖转运蛋白1（Glut1）的表达。一部分肿瘤组织被制成病理切片,经HE染色,观察光学显微镜下肿瘤细胞的坏死及萎缩情况。统计学处理采用SPSS11．0软件。多组样本均数之间比较采用单因素方差分析,标准摄取值（SUV）与Glut1表达行等级相关分析。结果随着放疗后时间的延长,^18F-FDG的摄取逐渐减少,代谢活性逐渐减低,SUV放疗前为1．28±0．31,放疗后第3天为1．00±0．23,放疗后第4周下降到0．18±0．10。放疗后各不同时间点肿瘤的平均SUV与放疗前的差异具有统计学意义（F=15．126,P〈0．05）。免疫组织化学结果：Glut1表达阳性的染色主要位于胞膜和胞质,随着放疗后观察时间的延长,肿瘤组织的Glut1表达逐渐减低,Glut1表达的平均吸光度（A）值放疗前为0．2558±0．03,放疗后第3天为0．2320±0．01,放疗后第4周为0．1320±0．04。肿瘤平均SUV与Glut1表达呈正相关（等级相关系数L=0．97,P〈0．01）。病理检查结果：随着放疗后观察时间的延长,肿瘤细胞萎缩、碎裂及坏死逐渐增多。结论PET／CT能于Wistar大鼠肺癌放疗后早期（第3天）观察到治疗效果,到第4周疗效显示最佳。     

PET/CT在评价及观察大鼠肺癌放疗效果中的应用

Objective The aim of this study was to understand the role of PET/CT in monitoring the therapeutic effect of radiotherapy (RT) on lung cancer with Wistar rats. Methods Thirty Wistar in-bred strain rats (6-8 weeks, weighed 180-280 g, female, ordinary) were made into Lewis pulmonary tumor model rats. 18F-fluorodeoxyglucose (FDG) PET/CT was performed when tumor reached 1.5-2.0 cm in greatest diameter (4-6 weeks) as a baseline. In order to get the optimal time point of PET/CT for moni-toring RT effect in rat cancer model, PET/CT was performed at the 3rd day, 1st, 2nd, 3rd, and 4th week after giving single dose of 5 Gy to each rat. Standardized uptake values (SUV) from FDG PET were measured and rats were sacrificed at different time point for validation. Besides, the expressions of glucose transport1 (Glut1) in tumor tissue were studied using immunohistochemistry. The level of tumor cell apoptosis, degra-dation, and necrosis were observed. SPSS 11.0 software was used for data analyses. Results A negative correlation of SUV uptake and time after RT and negative correlation of Glut1 expression and time after RT were observed in rat tumors, respectively. Positive correlation of SUV uptake and Glut1 expression in rat tumors was observed (Spearman rank correlation test, rs = 0.97, P < 0.01). Before RT, the SUV in rat tumor was 1.28 ± 0.31 and was decrease to 1. 00 ± 0.23 at the 3rd day and 0.18 ± 0. 10 at the 4th week after RT (F=15. 126, P<0.05). Before RT, the Glut1 in rat tumor was 0.2558 ±0.03 and was decrease to 0. 2320 ± 0. 01 at the 3rd day and 0. 1320±0.04 at the 4th week after RT. The amounts of tumor cell apopto-sis, degradation, and necrosis increased with time after RT. Conclusion Though FDG PET could monitor the therapeutic effect at the 3rd day after giving single dose of RT to rat lung tumor model, the optimal time was the the 4th week after treatment.