复合树脂充填物与酸蚀牙齿间密合度的扫描电镜观察

用SEM观察6种复合树脂充填物与酸蚀牙齿间密合度。结果表明6种复合树脂充填物与牙齿间均有微缝隙,其宽度多数在5μm内,最宽为10～15μm。1月后再观察,清晰见到从酸蚀牙本质小管曳出的树脂突,其曳出方向与缝隙形成可能有关。     

Hepatic Disposition Characteristics of Electrically Charged Macromolecules in Rat in Vivo and in the Perfused Liver

The effect of electric charge on the hepatic disposition of macromolecules was studied in the rat. Charged derivatives of dextran (T-70) and bovine serum albumin (BSA), mitomycin C–dextran conjugates (MMC-D), and lactosaminated BSA (Lac-BSA) were employed as model macromolecules. After intravenous injection, cationic macromolecules were rapidly eliminated from plasma because of their extensive hepatic uptake, while anionic and neutral macromolecules were slowly eliminated. Cationic macromolecules were recovered from parenchymal and nonparenchymal hepatic cells at a cellular uptake (per unit cell number) ratio of 1.4–3.2, while that of Lac-BSA was 14. During liver perfusion using a single-pass constant infusion mode, cationic macromolecules were continuously extracted by the liver, with extraction ratios at steady-state (E _ss) ranging between 0.03 and 0.54, whereas anionic and neutral macromolecules were almost completely recovered in the outflow at steady state. The E _ss for cationized BSA (Cat-BSA) and cationic MMC-Dcat were concentration dependent and decreased at low temperatures and in the presence of colchicine and cytochalasin B. The possible participation of the internalization process in the uptake of cationic macromolecules by hepatocytes was suggested.     

复方脑得生中总黄酮纯化工艺研究

目的研究复方脑得生中总黄酮的最佳纯化工艺。方法采用正交设计和单因素试验法，以总黄酮的洗脱率和含量为指标，考察利用D-101树脂富集、纯化复方脑得生中总黄酮的工艺条件及参数。结果优选纯化工艺为：上样溶液浓度0．1g／mL，流速2aV／h，树脂径高比1：7；吸附后，先用5BV的水洗脱，再用5BV40％乙醇洗脱。所得提取物中总黄酮的含量为80．57％，总黄酮转移率达83．76％。结论优选纯化工艺得到的总黄酮含量高，工艺简单，适宜工业化生产。     

大孔树脂富集三裂叶蟛蜞菊倍半萜内酯A和B的研究

目的 大量富集三裂叶蟛蜞菊倍半萜内酯A，B(WTA，WTB)。方法 采用气相色谱法测定WTA和WTB，并以WTA和WTB的含量、洗脱率、精制度为考察指标，研究D101大孔树脂吸附WTA和WTB的工艺条件及参数。结果 通过大孔树脂吸附后，WTA和WTB的洗脱率达90％，50％乙醇洗脱部分中WTA和WTB的含量为总固物的4．01％～4．09％。结论 可以采用大孔树脂富集WTA和WTB。     

诺氟沙星的吸附伏安法研究

在ｐＨ＝７．４９氨－氯化铵缓冲液中，诺氟沙星在滴汞电极上产生一灵敏的还原峰，峰电位为－１．５２Ｖ（ｖｓ．ＳＣＥ）。在１．９８×１０（－７）～４．８８×１０（－６）ｍｏｌ／Ｌ之间有良好的线性关系。本文对其极谱性质和电极过程进行了研究，用于诺氟沙星的测定，方法简便，结果准确、可靠。     

Patch testing with a mixture of 2 phenol-formaldehyde resins

1310 patients were routinely patch tested with a paratertiary-butylphenol-formaldehyde resin (PTBP-F-R), a resol resin based on phenol and formaldehyde (P-F-R-2), and a mixture of these 2 resins. Approximately 2.5 times more patients with contact allergy to phenol-formaldehyde resins were diagnosed when routinely patch tested with P-F-R-2 in addition to PTBP-F-R. Although patch testing with a mixture of both resins was not as good as patch testing with the 2 resins separately, it was better than testing only with PTBP-F-R, since 1.6 times more patients with contact allergy to phenol-formaldehyde resins were still diagnosed. P-F-R-2 is therefore recommended for routine patch testing, preferably as a separate patch test but otherwise as a mixture with PTBP-F-R.     

用酸蚀法提高纯钛与光固化冠桥树脂粘结强度的研究

目的：研究用酸蚀法提高钛与光固化复合树脂剪切粘结强度的可行性，并观察表面粗糙度与剪切粘结强度之间的关系。方法：采用不同浓度的酸蚀剂对钛铸件表面进行酸蚀，并与artglass光固化复合树脂进行粘结，以单纯喷砂组为对照，比较剪切粘结强度的差异。对酸蚀后及剪切试验后的金属表面进行扫描电镜观察，并测定其表面粗糙度。结果：用酸蚀法可以显著提高钛与复合树脂的粘结强度，HF浓度为4％时粘结强度最高。表面粗糙度随酸蚀剂的浓度增高而下降。结论：纯钛铸件用酸蚀法处理后可以获得较高的粘结强度，HF酸浓度以4％为宜。表面粗糙度对粘结强度不起决定性作用。     

Abnormal low density lipoproteins in children with familial hypercholesterolemia — Effet of polyanion exchange resins

Summary In 20 children and adolescents with familial Type IIa hyperlipoproteinemia, serum lipids and lipoproteins were examined before and during treatment with polyanion exchange resins. The composition of LDL was compared to that of healthy siblings. The patients were given Colestyramine (0.6 g/kg body weight) and Colestipol (0.5 g/kg body weight) in a cross-over study for 8 weeks each, after they had been under dietary treatment for at least 12 months. In 6 children, drug treatment had to be stopped due to side-effects. The most common complaints were gastrointestinal discomfort and constipation.Cholesterol, triglycerides and phopholipids were measured in whole serum and cholesterol, triglycerides and Apolipoprotein-B in isolated lipoprotein fractions after ultracentrifugation. Apo-B was determined by radial immunodiffusion.The Apo-B: cholesterol ratio in whole serum and in the LDL fraction was identical in the patients and in the controls. The LDL triglyceride: Apo-B ratio, however, was about 50% lower in the patients. This abnormal LDL composition was not altered by therapy with polyanion exchange resins. HDL cholesterol levels were significantly lower in the patients than in healthy children, and remained low during therapy.The decrease of total and LDL cholesterol (25%) and Apo-B (20%) was similar under both Colestipol and Colestyramine. Triglycerides and phospholipids showed no significant changes in therapy.These studies were supported by grants of the Schweizerische Nationalfonds and the Deutsche Forschungsgemeinschaft (Sonderforschungsbereich 90, Cardiovasculäres System)     

Adhesion dependent release of hepatocyte growth factor and interleukin-1 receptor antagonist from human blood granulocytes and monocytes: Evidence for the involvement of plasma IgG,complement C3 and β2 integrin

Objective:Evolving evidence of anti-inflammatory effects is observed in patients with rheumatoid arthritis or ulcerative colitis following periodic adsorptive granulocyte and monocyte (GM) apheresis with a column containing cellulose acetate (CA) beads as apheresis carriers. This study was undertaken to obtain insights into mechanisms of anti-inflammatory actions of adsorptive GM apheresis with CA beads. Methods:In a series of in-vitro experiments, we investigated the effects of plasma proteins and the leucocytes 2 integrin (CD18) on granulocyte adsorption to CA beads. Results:Granulocyte adsorption to CA beads required plasma IgG, the complement C3 and was inhibited by an antibody to leucocytes CD18. Further, hepatocyte growth factor (HGF) and interleukin-1 receptor antagonist (IL-1ra) which have strong anti-inflammatory actions were released by granulocytes that adhered to CA beads. Conclusions:Plasma IgG, C3 derived complement activation fragments and leucocytes CD18 are involved in granulocyte adhesion to CA beads and hence the release of HGF and IL-1ra.Received 27 October 2003; returned for revision 16 December 2003; accepted by M. J. Parnham 8 January 2004