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1.
雷公藤多甙对狼疮性肾炎患者血浆IL-13含量的影响   总被引:5,自引:0,他引:5  
目的 探讨雷公藤多甙(TW)对狼疮性肾炎(LN)患者血浆白介素-13(IL-13)含量的影响及治疗价值。方法 分别将激素诱导缓解治疗后的LN患者,按其活动指标分为活动期和非活动期2组,活动期患者加用TW治疗,观察治疗3个月后血浆IL-13水平的变化和肾炎的缓解情况。结果 血浆IL-13水平明显下降,尿蛋白减少,狼疮活动性免疫指标、血尿和肾功能改善。结论 TW可抑制血浆IL-13的产生,对控制LN的活动有一定的治疗价值。  相似文献
2.
急性痛风性关节炎中白细胞介素类细胞因子的研究进展   总被引:3,自引:0,他引:3  
痛风在我国的发病率呈上升趋势,其中痛风性关节炎是最常见的,它的急性发作是尿酸钠(MSU)微晶体在关节周围组织沉积引起的急性炎症反应。在此过程中MSU晶体作用于中性粒细胞、C54、滑膜细胞、T细胞、单核细胞、巨噬细胞、血小板,释放多种炎症介质,如炎性细胞因子、组织胺、前列腺素、血小板活化因子(PAF)、微血管增渗酶等。多种细胞因子通过自分泌和旁分泌的方式来影响痛风性关节炎的发生。  相似文献
3.
支气管哮喘患者IL-13的变化及意义   总被引:2,自引:0,他引:2  
目的:探讨IL-13在支气管哮喘(BA)发病中的意义。方法:采用固相酶联免疫分析(ELISA)法检测75例支气管哮喘患者血清IL-13水平。结果:支气管哮喘急性期患者血清IL-13水平较缓解期和健康对照组明显升高(P<0.01),而哮喘缓解期患者与健康对照组间无显著差异(P>0.05),外源性哮喘患者急性期血清IL-13水平明显高于内源性哮喘患者(P<0.05),而缓解期无明显差异(P>0.05)。结论:IL-13可能参与支气管哮喘的发病。血清IL-13对监测哮喘病情发展和转归有重要价值。  相似文献
4.
目的:探讨糖尿病肾病(DN)的发病机制以及消渴肾宁汤的作用机理.方法:以STZ腹腔内注射伴高热量饲养造DN模型,用消渴肾宁汤灌胃给药并和西药作对照,进行尿IL-13水平测定以及原位杂交检测.结果:实验性DN大鼠尿IL-13水平明显升高伴随严重损伤,消渴肾宁汤可显著降低IL-13水平改善肾损伤,疗效优于对照组.  相似文献
5.
目的:研讨银杏叶提取物(Egb)对哮喘大鼠信号转导和转录激活因子6(STAT6)、IL-13表达的影响,探讨其治疗哮喘的机制。方法:28只清洁级大鼠随机分为3组,对照组、模型组和Egb组,以卵白蛋白注射致敏雾化吸入激发法复制哮喘模型。用免疫组织化学法分别检测肺组织中STAT6蛋白表达水平,用流式细胞分析测定STAT6阳性细胞的表达率;用双抗体夹心酶联免疫吸附试验法测定支气管肺泡灌洗液(BALF)中IL-13浓度;对BALF进行细胞计数及嗜醵№垃细胞(EOS)分类计数。结果:①BALF中细胞总数和EOS占细胞总数的百分比(EOS%)模型组均显著高于对照组(P〈0.01),Egb组较模型组均显著降低(P〈0.01);②BALF中IL-13的浓度模型组显著高于对照组(P〈0.01),Egb组较模型组显著降低(P〈0.01);③模型组肺组织中STAT6蛋白表达和STAT6阳性细胞的表达率均较对照组明显增强(P〈0.01),Egb组较模型组明显减弱(P〈0.01)。结论:哮喘大鼠肺组织中STAT6蛋白有较强表达;Egb有抑制哮喘大鼠气道炎症的作用,其下调STAT6的表达、使IL-13分泌减少可能为其重要作用机制。  相似文献
6.

Aim of the study

The aerial part of Saururus chinensis has been used in folk medicine to treat several inflammatory diseases in China and Korea. Previously, our group reported that anti-asthmatic activity of an ethanol extract of Saururus chinensis (ESC) might occur, in part, via the inhibition of prostaglandin D2 (PGD2) and leukotriene C4 (LTC4) production, and degranulation reaction in vitro, as well as through the down-regulation of interleukin (IL)-4 and eotaxin mRNA expression in an in vivo ovalbumin-sensitization animal model. However, the effects of Saururus chinensis on eicosanoid generation, as well as Th2 cytokines and eotaxin production in an in vivo asthma model, have not been fully investigated. Moreover, it has not been determined whether ESC can ameliorate airway inflammation in vivo. In the present study, we investigated the therapeutic activity of Saururus chinensis on ovalbumin (OVA)-sensitized airway inflammation and its major phytochemical compositions.

Materials and methods

Asthma was induced in BALB/c mice by ovalbumin-sensitization and inhalation. ESC (10-100 mg/kg) or dexamethasone (5 mg/kg), a positive control, was administered 7 times orally every 12 h from one day before the first challenge to 1 h before the second challenge. The recruitment of inflammatory cells and hyperplasia of goblet cells were evaluated by H&E and PAS staining. Levels of Th2 cytokines, eotaxin, PGD2 and LTC4 were measured to evaluate the anti-inflammatory activity of ESC in OVA-sensitized mice. Contents of major components were analyzed by HPLC using a reversed-phase C18 column.

Results

ESC (10 mg/kg) suppressed allergic airway inflammation by inhibition of the production of IL-4 (P < 0.001), IL-5 (P < 0.05), IL-13 (P < 0.001), eotaxin (P < 0.001), PGE2 (P < 0.001), LTC4 (P < 0.001) in lung extract and IgE level (P < 0.001) in the serum. In addition, ESC (50 mg/kg) reduced the infiltration of inflammatory cells and hyperplasia of goblet cells in the lung tissues. The anti-inflammatory effect of ESC was comparable to that of the positive control drug, dexamethasone. Its major phytochemical composition includes manassantin A, B and sauchinone.

Conclusions

These results suggest that ESC decreased inflammation and mucus secretion in the OVA-induced bronchial asthma model, and its anti-asthmatic activity may occur in part via the inhibition of Th2 cytokines and eotaxin protein expression, as well as through prostaglandin E2 (PGE2) and leukotriene C4 (LTC4) generation. This effects may be attributed particularly to the presence of manassantin A, B and sauchinone major component evidenced by a HPLC analysis.  相似文献
7.
陈英群  马贵同  冯怡 《中医杂志》2007,48(7):638-640
目的探讨中药清肠泡腾栓治疗溃疡性结肠炎的免疫学疗效机制。方法实验分设正常对照组、模型对照组、空白泡腾栓组、清肠泡腾栓组、清肠栓组、柳氮磺胺吡啶栓组共6个组,ELISA法检测血清、结肠上清肿瘤坏死因子-α(TNF-α)、白细胞介素-13(IL-13);免疫组化、原位杂交检测白细胞介素8(IL-8)mRNA阳性表达。结果模型对照组血清TNF-α较正常对照组升高(P<0.05),结肠黏膜IL-8 mRNA阳性表达明显增强(P<0.01);清肠泡腾栓明显降低结肠上清TNF-α含量以及IL-8 mRNA阳性表达,与模型对照组比较差异有显著性意义(P<0.05或P<0.01);清肠泡腾栓对IL-13的调控作用不明显,与模型对照组比较差异无显著性意义(P>0.05)。结论清肠泡腾栓通过抑制促炎细胞因子而对溃疡性结肠炎产生治疗作用。  相似文献
8.
[目的]研究补脾益气方药对脾虚哮喘大鼠IL-13含量及IKKα表达的影响,揭示其平喘的作用机制。[方法]Wistar大鼠40只,分五组:对照组、脾虚哮喘组、哮喘组、脾虚哮喘中药组、哮喘中药组。ELISA检测大鼠支气管肺泡灌洗液(BALF)中IL-13含量;HE染色观察嗜酸性粒细胞(E0s)浸润情况;免疫组化观察肺组织中IKKα蛋白表达。[结果]与对照组比较,哮喘组和脾虚哮喘组IKKα表达及IL-13含量显著增高(P〈0.01),且脾虚哮喘组IKKα表达和IL—13含量均高于哮喘组(P〈0.05),中药治疗组和哮喘两组比较,IKKα表达和IL—13含量均降低(P〈0.05)。[结论]补脾益气方药能调控脾虚哮喘大鼠肺组织中IKKα的表达及BALF中IL-13的含量。  相似文献
9.
目的探讨痰热清注射液配合常规疗法治疗慢性阻塞性肺疾病(COPD)急性发作的疗效及其作用机制。方法将COPD急性发作期患者随机分为两组,均予西医常规治疗,联用组加用痰热清注射液,观察两组临床疗效并检测患者血清IL-13、LTB-4的含量和肺功能变化。结果联用组显效率明显高于常规组,对血清IL-13、LTB-4及肺功能的改善情况亦优于常规组。结论在常规疗法的基础上联用痰热清注射液治疗COPD急性发作能明显提高临床疗效,这可能与痰热清注射液能明显降低患者IL-13和LTB-4的分泌,减轻由其活化所致的炎症反应,改善肺功能有关。  相似文献
10.

Aim of the study

Lilium lancifolium is commonly used to treat bronchitis, pneumonia, etc. In this study, we investigated the anti-inflammatory effects of methanol extracts of the root of Lilium lancifolium (LL extracts) in LPS-stimulated Raw264.7 cells.

Material and methods

Levels of NO, PGE2 and pro-inflammatory cytokines (IL-6 and TNF-α) in the supernatant fraction were determined using sandwich ELISA. Expression of COX-2 and iNOS, phosphorylation of MAPK subgroups (ERK and JNK), and NF-κB activation in extracts were detected via Western blot and immunocytochemistry assays.

Results

The LL extract significantly inhibited NO, PGE2, IL-6 and TNF-α production in LPS-stimulated cells, and suppressed iNOS and COX-2 expression. A mechanism-based study showed that phosphorylation of ERK1/2 and JNK and translocation of the NF-κB p65 subunit into nuclei were inhibited by the LL extract. Furthermore, interleukin-4 and interleukin-13 production in Con A-induced splenocytes was suppressed.

Conclusion

These results indicate that anti-inflammatory effects of methanol extracts from Lilium lancifolium are due to downregulation of iNOS and COX-2 via suppression of NF-κB activation and nuclear translocation as well as blocking of ERK and JNK signaling in LPS-stimulated Raw264.7 cells.  相似文献
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