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木豆叶中黄酮微波提取工艺研究   总被引:5,自引:1,他引:4       下载免费PDF全文
金时  杨梅  孔羽  姚晓慧  魏作富  祖元刚  付玉杰 《中草药》2011,42(11):2235-2239
目的选取适合提取木豆叶中黄酮的方法,并优化提取工艺参数。方法以木豆叶中荭草苷、木犀草素和总黄酮的提取率及提取物抗氧化活性为指标,将微波辅助提取与热回流、超声波及浸泡提取方法相比较,并采用中心组合设计对影响荭草苷和木犀草素提取效果的微波辅助提取工艺参数进行了优化。结果通过比较确定微波辅助提取法较为适合木豆叶中黄酮的提取;优化得到荭草苷和木犀草素最佳微波提取工艺参数为:木豆叶粒径为50目,乙醇体积分数为80%,料液比1∶20,提取温度60℃,提取次数4次,提取时间10 min,微波功率500 W;荭草苷和木犀草素的提取率分别为(4.42±0.01)、(0.10±0.01)mg/g。结论采用微波辅助提取木豆叶中的黄酮具有提取时间短、提取率高、产物抗氧化效果好的特点,优化的工艺参数具有可行性和实际应用价值。  相似文献
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木豆叶水提物抗脑缺血缺氧损伤的作用研究   总被引:1,自引:1,他引:0  
目的探讨木豆叶水提物对脑缺血、缺氧损伤的保护作用。方法采用急性脑缺血再灌注模型法观察小鼠脑缺血后脑组织中超氧化物歧化酶(SOD)及过氧化脂质(MDA)的含量;采用急性脑缺血模型法观察大鼠脑缺血后脑组织的含水量、脑指数及脑毛细血管伊文思蓝的渗出量;采用小鼠断头法观察小鼠断头喘气时间。结果木豆叶水提物可显著降低急性脑缺血再灌注模型小鼠脑内MDA的含量,而SOD活力则显著提高,P<0.01;显著减少急性脑缺血模型大鼠脑组织的含水量及脑指数,P<0.05~0.01;显著减少急性脑缺血模型大鼠脑毛细血管伊文思蓝的渗出量,P<0.01;明显延长小鼠断头喘气时间,P<0.01。结论木豆叶水提物对大鼠、小鼠脑缺血、缺氧损伤均有一定的保护作用。  相似文献
3.
孙琳  柴智  张涛 《中草药》2016,47(2):297-300
目的 研究木豆叶提取物(ECCL)对H2O2诱导的H9c2细胞氧化应激损伤的保护作用及其机制。方法 建立H2O2诱导的H9c2细胞氧化损伤模型,于H2O2刺激前加入PI3K信号通路阻断剂LY294002(LY)预处理10 min,加入20、50 μg/mL ECCL预处理24 h。MTT法检测细胞存活率,比色法测定上清液中乳酸脱氢酶(LDH)、丙二醛(MDA)水平和超氧化物歧化酶(SOD)的活性,Western blotting检测细胞内p-Akt、p-eNOS蛋白表达。结果 与模型组比较,ECCL可明显增加H9c2细胞H2O2损伤后细胞存活率(P<0.01),增加SOD活力,降低MDA、LDH水平,增加p-Akt和p-eNOS蛋白表达(P<0.05、0.01),LY可阻断ECCL对H9c2的上述作用。结论 ECCL能够减轻H2O2所致的H9c2细胞损伤,可能是通过激活PI3K通路促进其下游因子Akt和eNOS磷酸化发挥作用。  相似文献
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目的:建立以药物活性和专属性菧类成分longistylin A与longistylin C为评价指标的树豆(木豆)药材质量控制方法.方法:采用硅胶柱色谱分离纯化有关成分,光谱技术鉴定化学结构;反相高效液相色谱法测定指标成分的含量.结果:从树豆叶分离鉴定了2种指标成分longistylin A和longistylin C;采用Thermo BDS Hypersil C18色谱柱(4.6 mm×250 mm,5μm),以甲醇-水(80:20)为流动相可使指标成分获得良好分离;longistylin A和longistylin C分别在0.002 88~0.057 6μg和0.011 2~0.224 μg呈良好的线性关系,平均回收率分别为98.9%,97.2%,RSD分别为2.4%,2.2%.结论:建立了以树豆叶中药物活性菧类成分为指标的药物分析方法,可用于树豆(木豆)药材的质量控制.  相似文献
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Ethnopharmacological relevance

There is only scant literature on the anticancer components of medicinal plants from Nigeria, yet traditional healers in the area under study claim to have been managing the disease in their patients with some success using the species studied.

Aim of study

To document plants commonly used to treat cancer in South-western Nigeria and to test the scientific basis of the claims using in vitro cytotoxicity tests.

Methods

Structured questionnaires were used to explore the ethnobotanical practices amongst the traditional healers. Methanol extracts of the most common species cited were screened for cytotoxicity using the sulforhodamine B (SRB) assay in both exposure and recovery experiments. Three cancer cell lines (human breast adenocarcinoma cell line MCF-7, human large cell lung carcinoma cell line COR-L23 and human amelanotic melanoma C32) and one normal cell line (normal human keratinocytes SVK-14) were used for the screening of the extracts and the fractions obtained. The extract of Cajanus cajan showed considerable activity and was further partitioned and the dichloromethane fraction was subjected to preparative chomatography to yield six compounds: hexadecanoic acid methyl ester, α-amyrin, β-sitosterol, pinostrobin, longistylin A and longistylin C. Pinostrobin and longistylins A and C were tested for cytotoxicity on the cancer cell lines. In addition, an adriamycin-sensitive acute T-lymphoblastic leukaemia cell line (CCRF-CEM) and its multidrug-resistant sub-line (CEM/ADR5000) were used in an XTT assay to evaluate the activity of the pure compounds obtained.

Results

A total of 30 healers from S W Nigeria were involved in the study. 45 species were recorded with their local names with parts used in the traditional therapeutic preparations. Cytotoxicity (IC50 values less than 50 μg/mL) was observed in 5 species (Acanthospermum hispidum, Cajanus cajan, Morinda lucida, Nymphaea lotus and Pycnanthus angolensis). Acanthospermum hispidum and Cajanus cajan were the most active. The dichloromethane fraction of Cajanus cajan had IC50 value 5–10 μg/mL, with the two constituent stilbenes, longistylins A and C, being primarily responsible, with IC50 values of 0.7–14.7 μM against the range of cancer cell lines.

Conclusions

Most of the species tested had some cytotoxic effect on the cancer cell lines, which to some extent supports their traditional inclusion in herbal preparations for treatment of cancer. However, little selectivity for cancer cells was observed, which raises concerns over their safety and efficacy in traditional treatment. The longistylins A and C appear to be responsible for much of the activity of Cajanus cajan extract.  相似文献
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