TGF-β诱导乳鼠上皮组织DNA甲基转移酶及相关miRNA表达的研究

目的 建立大鼠（乳鼠）肠上皮组织体外培养实验体系,检测TGF-β干预后大肠组织DNA甲基转移酶（DNA methyltransferase, Dnmt）及相关miRNA表达的变化,研究TGF-β诱导上皮组织的相关机制。方法 取出生3 d的SD大鼠大肠组织建立体外组织块培养体系,观察组织块生长的情况,再用10 ng/ml TGF-β干预组织生长,对比干预前后组织生长状况的变化,并在干预不同的时间取组织样本;HE染色观察组织结构变化,提取组织块中RNA,采用RT-PCR方法检测DNA甲基转移酶及其相关miRNA的表达水平变化。结果 TGF-β处理不同时间的组织块HE染色分析结果表明,在培养过程中,组织基本结构逐渐不能保持。对Dnmts及相关miRNA的表达水平检测结果表明,TGF-β干预组织后,Dnmts及相关miRNA的表达水平异常,相应升高或降低;在TGF-β干预过程中,使用甲基转移酶抑制剂5-Aza-dC干预24 h后,Dnmts的表达水平下降至无法检出,而此时相关miRNA的表达水平相应升高,甚至恢复至接近无TGF-β干预状态。结论 组织块培养过程中加入TGF-β干预,组织块的生长情况、Dnmts及相关miRNA的表达水平表现出异常,甲基化转移酶抑制剂能够抑制Dnmts的表达并且调节miRNA异常表达。     

301例脆性髋部骨折回顾性研究及文献共现分析

目的 分析确诊骨质疏松症后髋部骨折患者的发病特点及双膦酸盐类药物治疗史对院内治疗结局的影响。方法 回顾广州中医药大学第一附属医院一骨科10年间收治的确诊骨质疏松症后髋部骨折患者301例, 分析其发病特点,再按照是否有双膦酸盐类药物治疗史分为治疗组和未治疗组,对比两组患者住院特征差异,并开展文献研究,建立共现网络分析研究热点。结果 确诊骨质疏松症后继发髋部骨折人数占总髋部骨折人数的18.33%,好发于冬、春季,创伤原因多为低能量伤,中位发病年龄78岁,男女比1:4.68,治疗组患者发病年龄、住院天数、住院费用、手术率、手术等待时间显著低于未治疗组（P＜0.001）,两组患者的院内抢救率、院内死亡率及31天再住院率方面比较差异无统计学意义（P＞0.05）。结论 骨质疏松后脆性骨折的发生规律符合髋部骨折整体发病规律,但女性发病率明显增加。双膦酸盐类药物治疗史有利于缩短住院时间、降低住院费用、减少手术治疗率及手术等待时间,研究内容符合当前研究热点。     

伴代谢综合征急性胰腺炎的临床特征研究

目的 探讨伴代谢综合征的急性胰腺炎的临床特征.方法 回顾性分析我院2007年1月至2010年12月收治的33例伴代谢综合征的急性胰腺炎患者(MetS组)的临床资料,与同期住院的35例不伴代谢综合征的急性胰腺炎(非MetS组)资料进行对比研究.结果 MetS组腹痛缓解时间(7.88±3.89)天vs (4.67±2.64)天及平均住院时间(10.60±4.86)天vs (8.11±3.77)天,较非MetS组长,复发次数较非MetS组多(2.76±1.39)次vs( 1.22±0.53)次,死亡百分比高于非MetS组(3.0％ vs 0);MetS组患者血钙(1.67±0.48)mmol/Lvs (2.07±0.68) mmol/L、血氧分压水平(65±10)mmHg(l mmHg=0.133 kPa) vs (79±12) mmHg低于非MetS组,而CRP水平高于非MetS组(156.6±17.4) mg/Lvs (117.9±13.5) mg/L;MetS组中重症患者比例(30.3％ vs 14.3％)、Ranson评分≥3者(33.3％ vs 14.3％)、Balthazar CT分级为D、E级者(30.3％ vs 11.4％)、APACHE Ⅱ评分≥8分者比例(30.3％ vs14.3％)均较非MetS组高,MetS组局部及系统并发症均明显高于非MetS组(均P＜0.05).结论 合并代谢综合征的急性胰腺炎通常病情较重,并发症多,易复发,积极控制代谢综合征的各个组分可能对控制胰腺炎病情及预防胰腺炎复发有利.     

瑜伽防治慢性病的原理和研究进展

本文重点阐述了瑜伽对人体各系统和器官整合优化的功能,瑜伽体位法对防治疾病、消除紧张和疲劳的作用,瑜伽呼吸法对洁净呼吸系统、排除身体毒素、达到思想纯净的作用,瑜伽冥想镇静神经、减轻消极情绪作用。介绍了瑜伽防治痛经、心理疾病、心肺疾患、颈椎病、腰肌劳损、代谢综合症的研究成果。通过对瑜伽健身和防病的功能原理、防治慢性病的研究概况进行综述,以期寻找规律,为瑜伽应用于临床提供更多的依据。     

Microwave-assisted synthesis of ruthenium(ii) complexes containing levofloxacin-induced G2/M phase arrest by triggering DNA damage

Ru(ii) complexes have attracted increasing attention as promising antitumor agents for their relatively low toxicity, high affinity to DNA molecules, and correlation with multiple targets. Meanwhile, quinolones are synthetic antibacterial agents widely used in the clinical practice. In this paper, two novel Ru(ii) complexes coordinated by levofloxacin (LOFLX), [Ru(bpy)₂(LOFLX)]·2ClO₄ (1), and [Ru(dmbpy)₂(LOFLX)]·2ClO₄ (2) (bpy = 2,2′-bipyridine, dmbpy = 4,4′-dimethyl-2,2′-bipyridine) were synthesized with high efficiency under microwave irradiation and characterized by ESI-MS, ¹H NMR, and ¹³C NMR. The binding behavior of these complexes with double-strand calf thymus DNA(CT-DNA) was investigated using spectroscopy, molecular docking, and density functional theory calculations. Results showed that 2 exhibited higher binding affinity than 1 and LOFLX. Further studies showed that 2 could induce the G2/M phase arrest of A549 cells via DNA damage. In summary, these results indicated that 2 could be developed as a potential anticancer agent in treatment of lung cancer through the induction of cell cycle arrest at G2/M phase by triggering DNA damage.

This study showed that levofloxacin-based ruthenium(ii) complex 2 effectively inhibited the growth of A549 cells by inducing G2/M phase arrest through triggering DNA damage.     

“五脏相关学说”对多脏器功能不全诊治的指导价值

“五脏相关学说”认为人体可分为以肝、心、脾、肺、肾为主的五个子机能系统,它们之间生理上相互联系,病理上相互影响,而多脏器功能不全涉及到全身各个主要系统、脏器和组织,发病过程中相互影响,故认为以“五脏相关学说”为指导来诊治多脏器功能不全是有其临床意义的。文章从多脏器功能不全的发病机制及“五脏相关学说”与多脏器功能不全的内在联系方面进行了阐述。     

"五脏相关学说"对多脏器功能不全诊治的指导价值

"五脏相关学说"认为人体可分为以肝、心、脾、肺、肾为主的五个子机能系统,它们之间生理上相互联系,病理上相互影响,而多脏器功能不全涉及到全身各个主要系统、脏器和组织,发病过程中相互影响,故认为以"五脏相关学说"为指导来诊治多脏器功能不全是有其临床意义的。文章从多脏器功能不全的发病机制及"五脏相关学说"与多脏器功能不全的内在联系方面进行了阐述。     

Factors determining the side of approach for clipping ruptured anterior communicating artery aneurysm via supraorbital eyebrow keyhole approach

Purpose:The purpose of this study was to review the microsurgical anatomy and clipping of ruptured anterior communicating artery(AComA)aneurysms and to plan and avoid complications before operation.Methods:A total of 523 cases of cerebral aneurysms admitted to the neurosurgery department of the Third Affiliated Hospital of Sun Yat-Sen University from September 2010 to October 2018 were analyzed retrospectively.Among them,85 patients had ruptured AComA aneurysms.This study was limited to 85 of these cases,whose satisfactory preoperative angiographic diagnostic films can be retrieved from the hospital database system because of the need for detailed review.Results:We performed supraorbital eyebrow keyhole approach(SOEK)craniotomy in 85 patients to clip 85 AComA aneurysms,in the setting of subarachnoid hemorrhage(SAH).Patients’mean age was(52.69±9.94)years(range,28e78 years).The proportions of small,medium and large aneurysms were 83.5%,15.3%,and 1.2%,respectively.The average size of the aneurysms was(5.07±2.36)mm.There were 77.8% of patients with inferior aneurysms and 81.3% of patients with superior aneurysms achieved good results.There was a significant correlation between A1 dominance and operation method(p<0.001).There was no significant relationship between surgical approach and aneurysm projection or A2 plane(p=0.157&p=0.318).Conclusion:Regardless of whether the A2 plane is open or closed,the A1 dominant side is still a better choice for accessing AComA aneurysms to avoid dangerous premature bleeding.     

磁性附着体与冠外附着体联合应用的临床观察

目的探讨磁性附着体与冠外附着体联合应用的临床效果。方法对22例多数牙缺失伴残根残冠的患者,采用磁性附着体与冠外附着体联合修复治疗,随访2～4年,调查患者对义齿使用的满意情况,以及基牙和修复体的情况。结果患者对义齿的咀嚼功能、稳定性及美观性均满意。临床检查未发现基牙松动及继发龋,1例患者出现基牙周围骨吸收,5例患者出现基牙牙龈炎,1例患者修复2年后出现咀嚼痛,5例患者2年后出现义齿松动,经对症处理后义齿均继续正常使用。结论对于多数牙缺失并伴孤立残根残冠的患者,采用磁性附着体与冠外附着体的联合修复可获得良好的临床效果。