高效液相色谱法测定香薷药材中香芹酚和百里香酚的含量

目的 :建立香薷药材中香芹酚和百里香酚的含量测定方法。方法 :用高效液相色谱法 ,95 %乙醇超声提取 ,色谱柱InertsilODS-3,流动相甲醇 水 冰醋酸 (60∶40∶2 ) ,检测波长 274nm。结果 :香芹酚在 023～215μg (r=0.9999) ,百里香酚在 039～236μg (r =0.9999)有良好的线性关系 ;平均回收率分别为香芹酚 99.9% (RSD 1.4 % ) ,百里香酚 98.6 % (RSD 1.3% ) ,重复性的RSD分别为香芹酚 1.1% ,百里香酚 1.6 %。结论 :该方法可靠、便捷 ,可以作为控制香薷药材质量的方法。     

Metabolic response of Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) to essential oil of Ajwain and thymol

The effects of Carum copticum essential oil (CC EO) and thymol were assayed against fourth instar of Ephestia kuehniella Zeller by determining insecticidal activities and enzymatic responses. Concentrations of 5.42 and 6.81?μl/ml were obtained as LC₅₀ values for CC EO and thymol, respectively. The activities of esterases, aminotransferases, aldolase, and lactate dehydrogenase significantly decreased in the treated larvae by CC EO and thymol. Significant increases in glutathione S-transferases, γ-glutamyl transferase, and phosphatases were observed after treatment. Results revealed that CC EO and thymol significantly influenced the enzymatic activities of E. kuehniella leading to disruption of survival and metabolic responses.     

毛细管气相色谱法测定无极膏中薄荷脑、樟脑、水杨酸甲酯、冰片及麝香草酚的含量

目的:建立 GC 法测定无极膏中薄荷脑、樟脑、水杨酸甲酯、冰片及麝香草酚含量的方法。方法:采用 HP-5弹性石英毛细管柱程序升温的方法测定。结果:5组分 GC 法测定的分离度和线性关系良好,薄荷脑、樟脑、水杨酸甲酯、冰片、麝香草酚线性范围分别为132.4～1324.0μg·mL~(-1),243.9-2439.0μg·mL~(-1),132.0～1320.0μg·mL~(-1),20.0～200.0μg·mL~(-1),10.1～101.3μg·mL~(-1);平均回收率分别为99.8%(RSD=0.9%),99.5%(RSD=1.2%),98.7%(RSD=0.9%),99.5%(RSD=1.2%),100.8%(RSD=1.6%)。结论:本法准确、灵敏,可用于该制剂的质量控制。     

气相色谱法测定止痒消炎水中薄荷脑、冰片和麝香草酚的含量

目的建立同时测定止痒消炎水中薄荷脑、冰片和麝香草酚含量的气相色谱测定方法。方法采用气相色谱内标法,FID检测器,HP-INNDW AX石英毛细管柱(30m×250μm,0.25μm),应用程序升温(140℃保持4.1min以后20℃.min-1升至240℃),进样口温度200℃,检测器温度250℃;分流进样,分流比10:1。结果薄荷脑在0.32～3.2mg.mL-1,冰片在0.56～5.6mg.mL-1,麝香草酚在0.16～1.6mg.mL-1内呈良好的线性关系,加样回收率,薄荷脑为99.8%,(RSD=1.3%);冰片为99.2%(RSD=1.5%);麝香草酚为99.8%(RSD=0.9%)。结论该方法简单可靠,适合于止痒消炎水中薄荷脑、冰片和麝香草酚的含量测定。     

毛细管气相色谱法测定止痒消炎水中3种成分含量

目的:建立气相色谱法测定止痒消炎水中3种成分含量的方法。方法:采用 HP-INNOWAX Polyethylene Glycol 毛细管色谱柱(30.0 m×250μm,0.25 μm),柱温采用程序升温,进样口温度220℃,FID 检测器,检测器温度250℃。结果:样品中3种成分完全分离,线性关系良好,薄荷脑、冰片和麝香草酚的加样回收率分别为99.1%,99.3%,99.1%(n=9)。结论:该法简便快速,结果准确,可以有效地控制质量。     

直接滴定法测定麝香草酚原料的含量

目的：对采用直接滴定法测定麝香草酚原料含量方法学进行考察。方法：采用直接滴定法,通过重复性,线性等因素的考察建立符合麝香草酚原料含量测定的方法。结论：采用直接滴定法测定麝香草酚原料含量,操作简单,重现性良好（RSD=0．08％,n=6）,结果准确。     

Effect of two monoterpene phenols on antioxidant defense system in Candida albicans

Thymol and carvacrol from the class of monoterpene phenols are one of the most potent plant essential oil components possessing antimicrobial effects. Known for their wide bioactive spectrum, these positional isomers of isopropyl cresol deplete ergosterol content, compromise membrane permeability, block efflux pumps and restore antifungal susceptibility to fluconazole in resistant Candida strains. Exposure to these natural compounds induces a cascade of stress responses, which are important to comprehend their microbicidal mechanisms. This study evaluates the antioxidant defense response to lower concentrations of thymol and carvacrol in Candida albicans. The antioxidant defense responses in C. albicans are important for developmental mechanisms pertaining to resistance against the immune system, infection establishment and drug resistance. In this view, primary and secondary antioxidant defense enzymes, and oxidative stress markers including glutathione and lipid peroxidation were determined in C. albicans cells exposed to lower concentrations of thymol and carvacrol. These compounds were found to induce oxidative stress and compromised the antioxidant defense system in C. albicans at lower concentrations. This study helps in understanding the ‘in cell’ antifungal mechanisms of natural monoterpene phenols originating from oxidative stress. Thymol and carvacrol induced membrane deterioration reported earlier, is further explained as a result of a toxic radical cascade mediated by lipid peroxidation. Findings reinforce the observed toxic oxidizing effects of these compounds as a consequence of direct damage to antioxidant components and not to their genetic manipulations.     

毛细管气相色谱法测定复方麝香草酚搽剂的含量

目的建立气相色谱（GC）法同时测定复方麝香草酚搽剂中樟脑、麝香草酚含量的方法。方法采用DB-624弹性石英毛细管柱程序升温法。结果2组分能很好分离;樟脑、麝香草酚分别在0．2087～2．087、0．1140～1．140mg／ml浓度范围内呈良好线性关系;平均回收率分别为99．6％（RSD=0．9％）、99．6％（RSD=1．9％）。结论本方法准确、灵敏,可用于该制剂的质量控制。     

HPLC-RID法同时测定复方痱子粉中4种成分的含量

目的建立高效液相色谱-示差折光检测法测定复方痱子粉中樟脑、麝香草酚、薄荷脑和克霉唑4种成分的含量。方法采用示差折光检测器,C18色谱柱(150×4.6mm,5μm),以甲醇-水(78:22)为流动相,柱温30℃,流速为0.8mL/min,进样量为20μL。结果线性范围分别为樟脑0.210~0.631mg/mL,r=0.999 9;麝香草酚0.312~0.935mg/mL,r=0.999 8;薄荷脑0.207~0.621mg/mL,r=0.999 9;克霉唑1.389~4.166mg/mL,r=0.999 9。平均回收率为樟脑100.4%(RSD=0.5%),麝香草酚99.5%(RSD=0.7%),薄荷脑100.4%(RSD=0.6%),克霉唑100.3%(RSD=0.5%)。结论本法简便,快速,准确,可作为本品质量评价和生产工艺监控的有效方法。