墓头回诱导脐血基质细胞形成及分泌ＧＭ—ＣＳＦ的实验研究

目的　应用细胞培养技术检测了墓头回水提物对人脐血基质细胞生成及其分泌粒系造血刺激因子的实验研究。方法　将不同浓度的墓头回水提物与脐血共同培养14d,检测基质细胞上清液中的GM-CSF活性。结果　墓头回水提物对人脐血基质细胞具有促进形成的作用,最高峰为0.01μg/mL,为了检测基质细胞上清液中的GM-CSF活性,进行了GM-CFU实验,结果表明,实验各组数据与对照组比较,具有非常显著性差异(P＜0.01)。结论　墓头回水提物不仅是一种强烈的抗癌药物,也是一种造血祖细胞促进剂。     

白花败酱草化学成分研究

从白花败酱草石油醚和氯仿萃取部位分离鉴定了9个化合物,分别为正三十二碳酸(Ⅰ),正三十二烷醇(Ⅱ),棕榈酸(Ⅲ),齐墩果酸(Ⅳ),aurentiamide acetate(Ⅴ),胡萝卜苷(Ⅵ),β-谷甾醇(Ⅶ),7β-羟基-β-谷甾醇(Ⅷ),豆甾醇(Ⅸ),其中Ⅰ、Ⅱ、Ⅴ、Ⅷ是首次从败酱属植物中分离得到,Ⅳ、Ⅶ、Ⅸ是首次从白花败酱草中分离得到.     

The Anti-Inflammatory and Anti-Nociceptive Activities of Patrinia Villosa and Its Mechanism on the Proinflammatory Cytokines of Rats with Pelvic Inflammation

This study explores the anti-inflammatory and anti-nociceptive activities of Patrinia villosa, a Chinese medicinal plant, and to explore its effects on the proinflammatory cytokines of the rats with pelvic inflammation model. The animals were randomly divided into Patrinia villosa group (PV group), dexamethasone group (DEX group), and model-control group (CON group) to perform an ear edema test, a carrageenin-induced paw edema test, a cotton pellet-induced granuloma formation test, and an acetic acid-induced writhing test. The model rats with pelvic inflammation were established, and the serum levels of interleukin-6 (IL-6), interleukin-8 (IL-8) and tumor necrosis factor-alpha (TNF-α) in each group was detected with the Enzyme-Linked ImmunoSorbent Assay (ELISA). The results of the ear edema test, carrageenin-induced paw edema test, cotton pellet-induced granuloma formation test, and acetic acid-induced writhing test all showed that Patrinia villosa had strong anti-inflammatory and anti-nociceptive effects. In the experiment using model rats with pelvic inflammation, we found that the serum levels of IL-6, IL-8 and TNF-α in PV and DEX group were all significantly lower than those of the CON group, and the serum levels of IL-6 and IL-8 in PV group were significantly lower than those of the DEX group. Patrinia villosa, with its strong anti-inflammatory and anti-nociceptive activities, can be used to treat pelvic inflammation and to relieve the associated pain.     

Antitumor activity of polysaccharides isolated from Patrinia heterophylla

The research investigated the effect of Patrinia heterophylla Bunge (Valerianaceae) polysaccharides (PHB-P1) on U14-bearing mice. The tumor weight of mice treated with PHB-P1 (30, 60?mg/kg body weight) was significantly lower than that of the control group, a decrease of serum lactate dehydrogenase (LDH) activity was observed, and the serum alkaline phosphatase (AKP) level was increased slightly. The number of apoptotic tumor cells was significantly increased in the mice by treatment of PHB-P1 (30, 60?mg/kgbw). Cell cycle analysis showed the accumulation of tumor cells in the G2/M phase and a relative decrease of the S phase. By the immunohistochemical analysis, PHB-P1 (30, 60?mg/kgbw) might up-regulate the expression of p53 and Bax, and significantly inhibited the expression of Bcl-2 in tumor tissues. In conclusion, PHB-P1 could inhibit tumor growth and induce tumor cell apoptosis.     

基于计算机辅助药物设计的石荠苧抗流感药效物质多靶向作用研究简

目的 探讨石荠苧抗流感药效物质的多靶向作用机制.方法 以石荠苧中化学成分5-羟基-6,7-二甲氧基黄酮及其衍生物（MF1,MF2,MF3）为候选药物,采用计算机辅助药物设计方法研究其与流感血凝素蛋白（HA）、流感神经氨酸酶（NA）、炎症靶点环氧合酶-2（COX-2）和炎症靶点磷酸二酯酶4（PDE4）受体的对接程度.结果 在各候选药物与相关蛋白的对接中,木犀草素与HA活性位点的匹配度最高;鼠李柠檬素与NA和PDE4活性位点的匹配度最高;槲皮素与COX-2活性位点的匹配度最高;其中MF1,MF2,MF3也均表现出不同程度的匹配.结论 石荠苧中化学成分与流感病毒蛋白靶点和炎症相关蛋白靶点具有一定的结合和抑制效应,从而具多靶向抗流感病毒作用.     

白花败酱草乙醇提取物对小鼠抗氧化作用的影响

目的：研究白花败酱草乙醇提取物对小鼠抗氧化作用的影响。方法取昆明种小鼠32只,随机分为生理盐水组,5.0、10.0、20.0 mg/kg白花败酱草乙醇提取物组。连续给药10 d,次日小鼠拉颈椎脱臼处死,取心、脑、肝等组织,检测心、脑、肝等组织中的超氧化物歧化酶(SOD)的活性、丙二醛(MDA)的含量。结果10.0、20.0 mg/kg白花败酱草乙醇提取物能显著升高小鼠心、脑、肝等组织中SOD活性(P<0.05或P<0.01),显著降低心、脑、肝等组织中MDA含量(P<0.05或P<0.01)。结论白花败酱草乙醇提取物可清除体内脂质过氧化物,减轻机体的过氧化损伤,具有明显的抗氧化作用。     

威弛搽剂治疗家兔急性软组织损伤的实验研究

目的:研究糙叶败酱中环烯醚萜苷元成分体外抗小鼠结肠癌细胞株C26作用和对小鼠结肠癌(C26)移植性肿瘤的作用。方法:采用四唑盐(MTT)比色法研究糙叶败酱中环烯醚萜苷元PS-Ⅰ和BJ3401对小鼠结肠癌细胞株C26生长的影响;于F1小鼠皮下注射小鼠结肠癌细胞株(C26)建立小鼠结肠癌移植性肿瘤模型,观察糙叶败酱中总环烯醚萜苷元对结肠癌移植性肿瘤小鼠的影响。结果:环烯醚萜苷元PS-Ⅰ、BJ3401可显著抑制小鼠结肠癌细胞株C26的生长,并且具有一定的剂量依赖关系;总环烯醚萜苷元可显著减轻荷瘤鼠瘤重。结论:糙叶败酱中环烯醚萜苷元具有抗结肠癌的作用。     

龙胆总裂环环烯醚萜苷质量标准研究

目的：建立总裂环环烯醚萜苷（龙胆）的质量标准.方法：采用TLC（薄层色谱）法对提取物中的龙胆进行了定性鉴别;用紫外分光光度法测定了有效部位总裂环环烯醚萜苷的含量;用高效液相色谱法测定了龙胆苦苷的含量.结果：在TLC图谱中可检出龙胆的特征斑点;龙胆苦苷在7.187～35.94μg/ml间线性关系良好,r=0.9992,平均回收率为96.8%,RSD=1.06%（紫外分光光度法）;龙胆苦苷在0.8488μg～4.244μg间线性关系良好,r=.9997,平均回收率为97.3%,RSD=0.7%（高效液相色谱法）.结论：所建立的方法可行、重现性好,能有效地控制提取物中龙胆总裂环环烯醚萜苷的质量.     

白花败酱草黄酮类成分的高速逆流色谱快速制备

 目的应用高速逆流色谱分离纯化白花败酱草化学成分。方法利用正己烷-乙酸乙酯-甲醇-水(1∶0.6∶0.6∶1)为两相溶剂系统,主机转速800 r·min^-1,上相为固定相,下相为流动相,流速2.0 mL·min^-1,检测波长254 nm,所得产物经高效液相色谱法检测,结构经MS、¹H-NMR和13C-NMR鉴定。结果6 h内经一步洗脱从400 mg白花败酱草乙酸乙酯萃取物中分离得到纯度高于98%的木犀草素26 mg,5-羟基-7,4′-二甲氧基黄酮15 mg和5-羟基-7,3′,4′-三甲氧基黄酮21 mg。结论3个化合物均为首次从败酱属植物中分离得到,该分离制备法简便、快速、产物纯度高。     

中药龙胆主要病害及其防治

龙胆病害是影响龙胆产量和品质的重要原因,已成为制约龙胆药材生产的关键。本文就龙胆主要病害的病原菌、发生特点、病害症状、病害影响及防治方法等方面进行了综述,为更好的防治龙胆病害,提高龙胆的产量和品质提供参考。