2008年南京市疾病预防控制中心实验室间能力比对结果分析

[目的]通过外部措施评价实验室相应的检测能力,提高实验室检测水平,保证检测结果准确、可靠。[方法]依据国家标准GB/T4789-2003和2008年苏、浙、沪实验室间比对试验作业指导书(OTD)(微生物部分)结合ATB生化鉴定仪对模拟肛拭样品进行鉴定。[结果]经分离鉴定,该半固体模拟肛拭样品检出2株肠道致病菌,一株为小肠结肠炎耶尔森菌(血清型O3),一株为类志贺邻单胞菌。[结论]实验室间能力比对有助于促进实验室间技术交流与合作,选择性强、特异性高的显色培养其及快速生化鉴定条(ATB ID32E)使用,使检测更加快速、特异、敏感,取得满意结果。     

1株与宋内氏志贺氏菌血清发生交叉凝集的类志贺邻单胞菌

目的对引起食物中毒的病原菌进行分离鉴定,为疫情的防控提供依据。方法采取腹泻患者水样便直接划线平皿上获纯培养菌、血清学试验、系统生化鉴定的方法。结果分离到一株与宋内氏志贺氏菌诊断血清发生强凝集的类志贺邻单胞菌。结论在细菌的分离鉴定中,如果仅仅以血清学反应做出判定,容易产生错误的结论,还必须做系统的生化鉴定,以保证细菌鉴定的准确性,为疫情的防控提供科学、正确的依据。     

弧菌,气单胞菌及邻单胞菌编码鉴定研究

采用编码技术对医学常见的弧菌属、气单胞菌属及邻单胞菌属的１２ 个菌种进行编码,建立了７ 位数编码鉴定法。并用此法鉴定了２０８ 株标准菌株,与常规鉴定法比较,两法无显著统计学意义（χ２ ＝０－４４ ,Ｐ＞０－０５）,符合率为９５－６７％ 。此鉴定法快捷、可靠、操作简便、判定结果简单、适合于临床、卫生及基层微生物实验室推广应用。     

Plesiomonas Shigelloides Septicemia: Case Report and Literature Review

A 13-year-old girl with Plesiomonas shigelloides septicemia is reported. The infection occurred while she was receiving an allogeneic bone marrow transplantation for acute promyelocytic leukemia. Treatment with ciprofloxacin was successful. Twenty-one cases of Plesiomonas septicemia have been reported in the literature. Immunocompromised hosts, especially neonates, are commonly affected. The case mortality rate is high, with 13 of the reported patients dying of the infection. Successful treatment relies on the early identification of the organism and implementation of effective antibiotics.     

Congenital endophthalmitis following maternal shellfish ingestion

Purpose: To highlight an unusual organism causing a unilateral endophthalmitis by transplacental spread.
Method: We report a case of Plesiomonas shigelloides endophthalmitis, presenting in a newborn, with co-existing septicaemia and meningitis. There was a significant maternal history of diarrhoea associated with the ingestion of oysters 2 weeks prior to delivery.
Result: The endophthalmitis was treated with parenteral antibiotics and topical mydriatics with complete resolution, although subsequent assessment of the affected eye suggests a poor visual outcome.
Conclusion: Endophthalmitis in the newborn is an unusual clinical finding and usually presents with other manifestations of bacteraemia. Plesiomonas shigelloides is fortunately an infrequent cause of neonatal infection, but is associated with a high degree of morbidity and mortality. We postulate that this neonate acquired P. shigelloides via the transplacental route, and suggest that this organism be included in the list of 'other' causes of transplacental infection that has been abbreviated to 'O' in the acronym TORCH.     

类志贺邻单胞菌实时荧光TaqMan PCR 快速检测体系的建立

目的 建立针对类志贺邻单胞菌的高灵敏、高特异的实时荧光TaqMan聚合酶链式反应(PCR)快速检测体系.方法 根据类志贺邻单胞菌23S rRNA基因的一段特异性序列设计引物及TaqMan探针,利用实时荧光PCR检测平台探讨该检测体系的灵敏度;用30种其他肠道致病菌及院内感染中常见的致病菌评价该检测体系的特异性.结果...     

Pyosalpinx: not always a sexual transmitted disease? Pyosalpinx caused by Plesiomonas shigelloides in an immunocompetent host

Plesiomonas shigelloides are ubiquitous Gram-negative bacteria that are found in fresh or marine water, particularly in tropical or warm climates; they were recently implicated in diarrhoeal disease. Patients usually present with a history of recent travel to tropical regions or consumption of uncooked seafood. Extraintestinal disease has rarely been reported, occurring generally in neonates or immunocompromised patients, and is often fatal. We report a case of right pyosalpinx due to P. shigelloides acquired by swimming in contaminated water. Laparoscopic salpingectomy led to a good outcome.     

Prevalence of Plesiomonas shigelloides in aquatic environments

Plesiomonas shigelloides is a gram negative, facultatively anaerobic rod that has been implicated in a number of outbreaks of sporadic and epidemic gastroenteritis, particularly in tropical and subtropical areas of the world. It was isolated with high frequency from aquatic environments particularly fresh water and even drinking water. The present study was pursued to determine the incidence of the microorganism in tap and raw water samples, to correlate its presence with residual chlorine levels and to evaluate the seasonal effect on incidence. This information about these parameters is not available for the city of Caracas, Venezuela. Samples were streaked directly on inositol-brilliant green-bile salts agar (IBB) and Plesiomonas agar (PL). In addition, at least 100 ml of each sample was concentrated through a 0.45 mum Millipore membrane filter, which was enriched on alkaline peptone water (pH 8.6) during 24 h at 35 C. Enrichments were streaked on IBB and PL agar plates. All plates were incubated at 35 C for 24 h. Isolates were characterized using biochemical tests. The microorganism was not isolated from tap water samples. On raw water samples, a higher incidence was observed during the warmer season. Results indicated that water is a possible microorganism reservoir but the observed residual chlorine levels appear to be effective in controlling public health risk due to the microorganism's presence in drinking water.     

类志贺毗邻单胞菌感染的临床和流行病学特征

为进一步探索类志贺毗邻单胞菌在传染性腹泻和食物中毒中的地位和作用,我们于1984年10月至1985年10月对杭州市中医医院的腹泻病人和部分食物中毒病人进行了本菌的调查。
检测864份腹泻病人和12份食物中毒病人的粪便,发现12株类志贺毗邻单胞菌,阳性率1.37%。此外,还检出痢疾杆菌116株,副溶血性弧菌8株和致病性大肠杆菌2株。本菌感染者的临床症状类似细菌性痢疾的感染。其特征是腹泻呈水样或粘液便,次数较多,个别有里急后重,腹痛明显,1例有绞痛。部分患者有全身症状,如寒战、发热,2例达39℃。全部病例经抗生素和补液治疗,大多在3~5天内症状消失。5例病人病前2天内曾食用小水产品或海水产品,但同一家庭共同食用者未发生腹泻。该菌作为一种新的肠道致病菌,在我市腹泻和食物中毒的病原学中已占有一定地位,应引起关注,并进一步探索了本菌感染的致病机理和防治措施。     

PLESIOMONAS SHIGELLOSES SEPTIC ARTHRITIS COMPLICATING RHEUMATOID ARTHRITIS

A patient with severe seropositive rheumatoid arthritis and hepatic cirrhosis developed septic arthritis of his knees. Plesiomonas shigelloides was isolated from joint fluid, blood, and also from the gut. The patient's joint symptoms responded to treatment with oral trimethoprim-sulphamethoxazole, but he died of uncontrolled gastrointestinal bleeding five days later.