What the structure of angiostatin may tell us about its mechanism of action

Summary.  Originally discovered in 1994 by Folkman and coworkers, angiostatin was identified through its antitumor effects in mice and later shown to be a potent inhibitor of angiogenesis. An internal fragment of plasminogen, angiostatin consists of kringle domains that are known to be lysine-binding. The crystal structure of angiostatin was the first multikringle domain-containing structure to be published. This review will focus on what is known about the structure of angiostatin and its implications in function from the current literature.     

民族医药移动信息平台设计与实现

以广西中医药大学已建成的民族医药专业数据库作为依托，基于微信公众平台设计和实现一个功能完善、简单易用的民族医药移动信息平台。介绍平台设计思路、设计原则、功能模块、关键技术、预期结果等方面。     

基于云计算的医疗卫生位置服务平台研究

对位置服务(LBS)和云计算分别进行介绍,在此基础上提出基于云计算的医疗卫生LBS平台模型和架构,并对其在医疗卫生领域的具体应用进行探讨,包括医疗卫生机构网点查询、紧急救援、人员设备管理、流行病监控及突发事件应急、社区医疗卫生信息管理等.     

Inhibition of plasmin-mediated prostromelysin-1 activation by interaction of long chain unsaturated fatty acids with kringle 5

C18 unsaturated fatty acids were here found to inhibit proMMP (matrix metalloproteinase)-3 activation by plasmin. This effect was suppressed by lysine ligand competitors, indicating that it was mediated by binding to kringle domains. Surface plasmon resonance analysis demonstrated that oleic acid interacted to a similar extent with plasmin and kringle 5 (KD values of 3.4 x 10(-8) and 5.9 x 10(-8)M) while interaction with kringles 1-2-3 was 10-fold lower. Furthermore, oleic acid stimulated the amidolytic activity of plasmin and mini-plasmin, but not micro-plasmin. Oleic acid also enhanced u-PA (urokinase-type plasminogen activator)-mediated plasminogen activation over 50-fold. Taken together, these data indicate that inhibition of plasmin-induced proMMP-3 activation by unsaturated fatty acids was mediated through their preferential binding to kringle 5. The influence of elaidic acid on the plasmin/MMP-3/MMP-1 proteolytic cascade was assessed ex vivo. Exogenous addition of plasmin to dermal fibroblasts or supplementation of gingival fibroblast culture medium with plasminogen triggered this cascade. In both instances, elaidic acid totally abolished proMMP-3 and proMMP-1 activation. Additionally, a significant decrease in lattice retraction and collagen degradation in a range similar to that obtained with Batimastat was observed when human gingival fibroblasts were cultured in plasminogen-containing type I collagen gels, indicative of the dual influence of unsaturated fatty acids on MMP activation and activity. In conclusion, unsaturated fatty acids or molecules with similar structures could be attractive target for the development of natural pharmacological inhibitors directed against plasmin and/or MMPs in different pathological contexts such, skin UV irradiation, vascular diseases and tumour growth and invasion.     

The binding of plasminogen to fibrin: Evidence for plasminogen-bridging

The ability of plasminogen to cause precipitation of soluble fibrin oligomers has been observed and certain features of the phenomenon investigated. The process is mediated by the lysine-binding sites and it appears that at least two such sites are required. Studies using radiolabelled plasminogen revealed that the precipitated material contained fibrin and plasminogen in a 2:1 molar ratio. Further plasminogen molecules are able to bind to the aggregate. The clotting of fibrinogen in the presence of plasminogen was studied using nephelometry. An enhancement by plasminogen of both the rate of clotting and the opacity of the clot was demonstrated. It is proposed that these effects are explicable in terms of a plasminogen-bridging model, in which the zymogen binds divalently between two monomer units of forming polymeric fibrin.     

不同班级环境类型中职生学业行为差异比较研究

目的探讨班级环境类型对中职生学业行为的影响。方法采用班级环境和学业行为问卷对宝鸡市384名中职生进行调查。结果①中职生班级环境的师生关系、同学关系维度存在显著的性别差异(t=-3.863,-3.209,P<0.01)、秩序纪律维度存在显著的年级差异(t=-2.502,P<0.05)、师生关系、竞争气氛维度存在显著的家庭经济条件差异(t=4.152,3.602,P<0.05);②班级环境中的同学关系能够显著的预测学业行为;③不同班级环境类型的中职生在学业行为上具有显著差异。结论不同班级环境类型中职生学业行为存在显著差异,班级同学关系对学业行为的影响更为显著。     

温和灸腹部募穴对实验性大鼠肠道菌群失调的影响

目的通过温和灸关元、天枢两个腹部募穴,观察其对肠道菌群失调的影响。方法清洁级Wistar大鼠50只,雌雄各半,体重（200±20）g,采用随机对照方法分为正常组（A）、模型组（B）、药物组（C）、关元组（D）、天枢组（E）,每组10只。用大量盐酸林可霉素灌胃造模,造模成功后,分别进行药物治疗和温和灸法治疗。1个疗程后,各组大鼠均被迫采取新鲜粪便0.1 g,应用双歧杆菌（BS）、乳酸杆菌（LBS）、肠杆菌（EMB）、肠球菌（EC）选择性培养基进行细菌培养,生化鉴定管和比浊法检测不同菌落生长情况和各组菌落数量。结果温和灸关元穴使BS、LBS数量有所增加,温和灸天枢穴使EMB、EC数量有所增加。结论温和灸不同部位的募穴可以选择性调整肠道优势益生菌群,从而治疗肠道菌群失调症。     

药物依赖患者行为干预与心理援助系统的研制

目的:针对药物依赖患者回归社会在帮教环境缺失环境下很容易复吸的问题,本文介绍了一种基于行为治疗的药物依赖治疗设备的实现。方法:该系统由智能手机、语音呼叫识别器和远端计算机构成。以该设备为手段实时了解患者行为情况,必要时提供人工心理疏导,规范药物依赖患者作息和活动的模式。结果:系统已经实现手机佩戴患者语音识别,位置定位,高危区域提示、患者病历管理功能。结论:该设备的研制为戒毒综合治疗提供了新的方法,有望促进患者戒断成功。     

C3b acceptors on macrophages: inhibition of Fc gamma-receptor-mediated phagocytosis by acceptor-bound C3b

The binding of nascent human C3b (i.e. the fragment of C3 just after trypsin cleavage) to mouse peritoneal macrophages was demonstrated by immune adherence. Acceptor-bound C3b could be detected longer than 24 h on the cell membrane. The rosette formation and phagocytosis of SRBC coated with anti-SRBC rat IgG was inhibited by preincubation of the cells with C3 and trypsin (15 min, 37 degrees C). However, the phagocytosis of opsonized yeast particles was not influenced by acceptor-bound C3b, proving that C3b-C3b acceptor interaction did not alter the function of C3b-receptors. Acceptor-bound C3b on the macrophages failed to mediate phagocytosis of human 0,Rh+ red cells having C3b-receptors.