薄荷组织培养研究

目的采用组织培养的方法,进行薄荷种苗快速繁殖技术研究,为其种苗生产的现代化提供科学依据。方法用不同浓度和配比的6-BA和NAA以及适当调节铁离子浓度对薄荷茎段进行组织培养。结果以6-BA:NAA为4:0.2ppm配比时芽丛诱导数最多,同一材料粗细差异极其显著的母本茎段接种于不同浓度和配比的6-BA和NAA组合的培养基上培养三代后,其后代无显著粗细差异,但粗茎段母本的后代在生长性状上优于细茎段母本;试验同时表明,铁离子浓度过高会抑制芽的生长。结论适宜的芽增殖培养基:4ppm6-BA+0.2ppmNAA。     

Bioactivity studies on beta-sitosterol and its glucoside

Beta-sitosterol and beta-sitosteryl-beta-D-glucoside were isolated as analgesic constituents from the leaves of Mentha cordifolia Opiz. The acetic acid-induced writhing test showed that beta-sitosterol and beta-sitosteryl-beta-D-glucoside decreased the number of squirms induced by acetic acid by 70.0% and 73.0%, respectively, at a dose of 100 mg / kg mouse. Statistical analysis using the Kruskall Wallis one-way analysis of variance by ranks showed that these isolates approximate the analgesic activity of mefenamic acid at a 0.001 level of significance. The hot plate method confirmed their analgesic activities, as beta-sitosterol and beta-sitosteryl-beta-D-glucoside exhibited a 300% and 157% increase in pain tolerance, respectively, while mefenamic acid, a known analgesic, showed a 171% increase. Neither isolate exhibited antiinflammatory activity using the carrageenan-induced mouse paw oedema assay. Beta-sitosterol also exhibited anthelminthic and antimutagenic activities. In vitro tests using live Ascaris suum as test animals showed that the behaviour of worms treated with beta-sitosterol approximated that of the positive controls, Combantrin and Antiox. An in vivo micronucleus test showed that beta-sitosterol inhibited the mutagenicity of tetracycline by 65.3% at a dose of 0.5 mg /kg mouse. At the same dose, it did not exhibit chromosome-breaking activity.     

鲜薄荷外用治疗小儿红痱疗效观察

痱（miliaria）亦称为粟粒疹,是由于高温闷热环境中出汗过多不易蒸发致使汗腺导管口堵塞,汗液储藏后汗管破裂而引起汗液外溢渗入周围组织的浅表性炎症反应。包括四种类型：白痱或晶形粟粒疹（miliaria crystallina）,红痱或红色粟粒疹（miliaria rubia）,脓痱或脓胞性粟粒疹（miliaria pustulosa）和深痱或深部粟粒疹（miliaria profunda）[1]。     

镉低积累药用植物盆栽及大田筛选试验

目的 比较不同植物对重金属镉积累能力差异,筛选出镉低积累药用植物种类.方法 以夏枯草、益母草、薄荷为供试材料,通过盆栽和大田试验,采用原子吸收光谱法测定植物中镉的含量.结果 三种植物积累镉的能力存在差异,益母草>薄荷>夏枯草.益母草在镉污染土壤中其地上部分镉含量随土壤镉浓度增高而增高,而生长不受影响,属于抗性植物.在镉胁迫下,薄荷、夏枯草生长受到抑制,植物中镉含量较低,且随土壤镉浓度增加植物中镉含量变化较小,属于镉低积累植物.结论 益母草为镉高积累植物,且生长不受影响,可以作为富集植物治理镉污染;薄荷中镉主要积累在地下部分,而其以地上部分入药,夏枯草为镉低积累植物,两者均为可在镉污染地区种植的药用植物.     

Green synthesis of tea Ag nanocomposite hydrogels via mint leaf extraction for effective antibacterial activity

In this report, we investigated the swelling behavior and antibacterial property of nanosilver composite hydrogels made from tea with polyacrylamide via a free-radical polymerization and green process technique. This is probably for the first time; tea-based nano silver composite hydrogels were developed. The composite hydrogels comprise embedded nano silver particles in the tea hydrogel matrix via a green process with mint leaf extract. The size of the nano silver particles in the hydrogel matrix was found to be < 10 nm. The nano silver composite hydrogels formed and their blank hydrogels from the mint leaf were characterized by using ultraviolet-visible spectroscopy, scanning electron microscopy with energy dispersive spectroscopy, transmission electron microscopy, thermogravimetric analysis and X-ray diffraction studies. The nano silver composite hydrogels developed exhibit eminent antibacterial activity against Escherichia coli and Staphylococcus aureus. This clearly indicates that the nano silver composite hydrogels are potential candidates for antimicrobial applications.     

Modulation of alveolar macrophage innate response in proinflammatory-, pro-oxidant-, and infection- models by mint extract and chemical constituents: Role of MAPKs

There is a continuing need for discovering novel primary or adjunct therapeutic agents to treat inflammatory conditions and infections. Natural products have inspired the discovery of several modern therapeutics; however, there is a paucity of mechanistic information on their mode of action. This study investigated the therapeutic potential and mode of action of corn mint’s (Mentha arvensis) leaf extract (ME) in alveolar macrophages (AMs) challenged with model pro-inflammatory (LPS), pro- oxidant (LPS or H₂O₂), and infection (Mycobacterium) agents and contribution of its dominant constituents rosmarinic acid, l-menthol, and l-menthone. LPS-induced inflammatory response in the murine AM cell line MH-S was significantly reduced in terms of pro-inflammatory cytokines (TNF-α, IL-1α) and nitric oxide (NO) when pre- or post-treated with ME. The ME pretreatment of macrophages led to a significant increase ( P≤ 0.05) in phagocytic activity toward Mycobacterium smegmatis and a greater pathogen clearance in 24 h in both ME pre-treated (P ≤ 0.05) and post-treated cells. Significant attenuation (P ≤ 0.01) of reactive oxygen species (ROS) production in LPS- or H₂O_2-treated macrophages by pretreatment with whole mint extract (ME) was accounted for in part by the mint constituents rosmarinic acid and l-menthone. Attenuation of pro-inflammatory response by ME pretreatment coincided with the significant reduction in total and phosphorylated JNK1/2, decrease in total p38, and increase in phospho-ERK1/2 thereby implying a role of differential modulation of MAPKs. Taken together, the results demonstrate that corn mint leaf components cause potent anti-inflammatory, anti-oxidant, and anti–infection effects in AMs via suppression of the production of cytokines/soluble mediators and ROS and increased pathogen clearance, respectively. To our knowledge, this is the first report on the mode of action of corn mint targeting the alveolar macrophages and on the potential role of MAPKs in immunomodulation by this product.     

仝小林运用炒栀子、连翘、薄荷清鼻窍经验

仝小林认为鼻炎的治疗当辨涕之脓清、辨有无过敏。当其辨证要点为黄浓涕时,仝院士常精选三味药物——炒栀子、连翘、薄荷合成三味小方,炒栀子泻火除烦,清热解毒,连翘清热解毒,疏散风热,薄荷疏散风热,清利头目,利咽透疹,三者以清上焦之热为主,共奏清鼻窍之功,在鼻炎的治疗中具有较好的疗效。该小方临床常用剂量分别为薄荷6~15 g,炒栀子、连翘15~30 g。     

维生素B_1穴位注射联合山薄荷热湿敷对急性胰腺炎肠麻痹肠功能恢复的影响

目的探讨维生素B1穴位注射联合山薄荷加酒热湿敷对急性胰腺炎肠麻痹肠功能恢复的影响。方法采用随机数字表法,将120例患者分为观察组和对照组,每组各60例。对照组采用常规治疗和护理,观察组在对照组基础上给予维生素B1穴位注射联合山薄荷加酒腹部脐周热湿敷（40～43℃）。观察两组患者肛门首次排气和排便时间的差异。结果观察组患者肛门首次排气和排便时间较对照组提前,两组比较,均P〈0.01,差异具有统计学意义。结论维生素B1穴位注射联合山薄荷加酒热湿敷能促进急性胰腺炎胃肠道的蠕动,促进机体早日康复。