金霉素眼膏对胃肠减压患者鼻咽舒适度的影响

目的：探讨金霉素眼膏鼻腔局部用药对胃肠减压患者鼻咽部舒适度的影响。方法：选取2012年7月-2013年10月本科留置胃肠减压管的182例患者,按照随机数字表法将其分为对照组90例和干预组92例。对照组按常规要求进行口腔护理、漱口液漱口、生理盐水棉签清洁鼻腔;干预组在对照组的基础上使用金霉素眼膏在插胃管侧鼻腔局部用药。比较两组患者鼻咽部舒适度及患者对胃管的接受程度。结果：干预组的鼻咽部不适发生率67.40%（62/92）明显低于对照组的96.67%（87/90）,对胃管接受程度明显高于对照组,差异均有统计学意义（P〈0.01）。结论：胃肠减压患者采用金霉素眼膏鼻腔局部用药可减轻鼻咽部不适,增加患者对胃管的接受程度,提高患者舒适度与治疗依从性,值得临床推广。     

食品虾仁中土霉素、四环素、金霉素含量测定方法的建立

目的：建立高效液相色谱法测定虾仁中土霉素、四环素、金霉素的含量。方法：采用C18色谱柱（250 mm×4.6 mm,5 μm）;梯度洗脱,以甲醇为流动相A,乙腈为流动相B,10 mmol·L^-1三氟乙酸为流动相C;流速1.5 mL·min^-1;检测波长为350 nm。结果：土霉素、四环素、金霉素检出限分别为0.005、0.005和0.020 mg·kg^-1,土霉素、四环素线性范围为0.05~1.00 μg·mL^-1,相关系数为0.9996及0.9995,金霉素线性范围为0.20~4.00 μg·mL^-1,相关系数为0.9998;加标回收率为73.5%~94.2%。结论：本方法简便、灵敏、专属,重复性好,可用于同时测定虾仁中土霉素、四环素、金霉素的含量。     

健脾除湿祛风方加减治疗慢性唇炎44例

目的:观察健脾除湿祛风方加减治疗慢性唇炎的临床疗效。方法:选取2012年1月—2014年1月黄冈市中医医院治疗的慢性唇炎患者88例为研究对象,随机分成对照组和观察组,每组44例。对照组给予金霉素软膏涂抹唇部治疗,观察组在对照组治疗的基础上加用健脾除湿祛风方治疗。结果:对照组有效率81.82%,观察组有效率93.18%,观察组优于对照组(P0.05);观察组治疗后临床症状、体征评分均优于对照组(P0.05);对照组治疗后3个月、6个月、12个月复发率分别为18.18%、29.55%、45.45%,观察组治疗后3个月、6个月、12个月复发率分别为6.82%、13.64%、20.45%,观察组优于对照组(P0.05)。结论:健脾除湿祛风方加减治疗慢性唇炎临床疗效显著,复发率低。     

45Ca2+ uptake by dispersed pancreatic islet cells: Effects ofd-glucose and the calcium probe,chlorotetracycline

Uptake of⁴⁵Ca²⁺ was studied in dispersed pancreatic islet cells from non-inbredob/ob-mice. Like whole islets the dispersed cells responded to 20 mMd-glucose with a markedly increased⁴⁵Ca²⁺-labeling of both the lanthanum-nondisplaceable and the lanthanum-displaceable calcium pools. The pronounced effect ofd-glucose could not be reproduced with 3-O-methyl-d-glucose,l-glucose,d-mannose,l-leucine, ord-leucine; however,⁴⁵Ca²⁺ uptake was greater in the presence ofl-leucine as compared withd-leucine.⁴⁵Ca²⁺ uptake by dispersed cells or whole islets was stimulated severalfold by 100 M or more chlorotetracycline. At the concentration of only 10 M, chlorotetracycline had no effect on whole islets and partially inhibited⁴⁵Ca²⁺ uptake by the dispersed cells. The ability ofd-glucose to stimulate⁴⁵Ca²⁺ uptake by islets or dispersed cells remained in the presence of 10 M chlorotetracyline. Islet cell suspensions apparently represent a valid model for studying how Ca²⁺ interacts with the cells. However, when using chlorotetracycline as fluorescent Ca²⁺ probe, attention must be paid to its potential ionophoric activity. At only 10 M, the drug seems to monitor a peripheral pool of Ca²⁺, some of which may reside in normal transport channels.