雷公藤多甙对大鼠胶原免疫性关节炎黏膜免疫的影响

目的:观察雷公藤多甙对于大鼠胶原免疫性关节炎黏膜免疫的影响.方法:Ⅱ型胶原与完全弗氏佐剂免疫大鼠,以雷公藤多甙治疗,观察大鼠黏膜免疫各项指标.结果:雷公藤多甙可以改善CIA大鼠病理改变,降低肠道PP结中CD4/CD8比值,对脾中T细胞亚群有调节作用,并可降低关节中致炎细胞因子的表达.结论:雷公藤多甙对CIA模型大鼠有抗炎和免疫抑制的作用.     

Rate of intrusion of maxillary incisors in Class II Div 1 malocclusion using skeletal anchorage device and Connecticut intrusion arch

BackgroundNonsurgical correction of deep bite involves either extrusion of posterior teeth, intrusion of incisors, or combination of both. The introduction of skeletal anchorage device with microimplant provides near absolute anchorage without producing any untoward effects on anchor unit. Connecticut Intrusion Arch (CIA) provided an efficient system of intruding anterior segment without producing much adverse affects on anchor teeth.MethodsThe study comprised of 30 patients of Class II Div 1 malocclusion with overbite of >6 mm and required therapeutic extractions of all first premolars, randomly distributed into two groups. Group 1 was treated using orthodontic microimplants, while Group 2 treated with CIA. Lateral cephalograms were taken pre-intrusion (T1) and post-intrusion at the end of six months (T2).ResultsThe rate of intrusion was 0.51 and 0.34 mm/month for Group 1 and Group 2 respectively. The average amount of change in centroid point to PP distance and U1-SN angle was significantly higher in Group 1 compared to Group 2 (P < 0.001). The average amount of change in U6 to PP distance did not differ significantly between two study groups (P > 0.05).ConclusionThe amount of intrusion is significantly higher in SAD group. Although vertical molar positional change was higher in CIA group than the SAD group, it was not changed significantly in both treatment modalities. SAD group overall had better results and was easier in handling during intrusion.     

Tartrate-resistant acid phosphatase-positive cells in the synovial–cartilage junction and bone marrow during the progression of collagen-induced arthritis in adult rats

We investigated the time-course changes in bone destruction in rats with collagen-induced arthritis (CIA). The synovial–cartilage junction (SCJ) and epiphyseal bone marrow of the femoral posteromedial condyle in CIA rats were evaluated histologically and immunohistologically at 2, 3, 4, 6, and 8 weeks after sensitization. Two weeks after sensitization, tartrate-resistant acid phosphatase (TRAP)-positive multinuclear cells formed resorption lacunae on the lateral side of the cortical bone under the SCJ. No resorption lacunae were observed in bone marrow. Three weeks after sensitization, resorption lacunae on the lateral side of the cortical bone expanded, but no bone marrow invasion by pannus was observed. In bone marrow, many TRAP-positive multinuclear cells appeared and formed resorption lacunae in subchondral bone. Four weeks after sensitization, cortical bone was destroyed, and pannus had invaded the bone marrow. After six weeks, trabecular bone and subchondral bone plate were extensively resorbed by TRAP-positive cells. Bone destruction in CIA began with the appearance of TRAP-positive cells on the lateral side of the cortical bone under the SCJ, followed by the TRAP-positive multinuclear cells in bone marrow, which were morphologically unconnected to the SCJ lesions. These histological findings suggested that bone destruction in the early stage of arthritis occurred in two anatomically different regions.     

什么是合理的医疗卫生体系

医疗卫生体系是一个复杂的体系,医疗卫生体制改革也必然是一个复杂的系统工程。医疗卫生体系由医疗卫生保障体系、医疗卫生服务体系、医疗卫生资源提供体系及医疗卫生监管体系4个紧密相关的子系统构成。一个合理的医疗卫生体系应该是以人民健康为中心,在医疗卫生服务的提供与保障上兼顾公平与效率为原则的。建设合理有效的医疗卫生体系,其关键是抓好体系的能力建设、动力机制建设以及压力机制建设。     

中药熏蒸疗法对Ⅱ型胶原蛋白诱导型关节炎大鼠关节病理改变的影响

目的 研究中药熏蒸疗法对Ⅱ型胶原蛋白诱导的关节炎(CIA)模型大鼠关节病理改变的影响.方法 用小牛源Ⅱ型胶原复制CIA模型.清洁级雄性Wistar大鼠随机分为正常组、模型组、水熏组、低熏组、高熏组,正常组和模型组常规喂养,不作熏蒸处理;水熏组给予(57±1)℃蒸馏水熏蒸,蒸气温度为(39±1)℃;低熏组给予水熏组相同温度的3.33%中药液熏蒸;高熏组给予水熏组相同温度的6.67%中药液熏蒸,造模第41日处死大鼠,获取完整膝关节.光镜下观察炎性细胞浸润、纤维组织增生及滑膜细胞增生情况.结果 中药熏蒸疗法对CIA大鼠骨关节组织的炎性细胞浸润、纤维组织增生及滑膜细胞增生有明显抑制作用.3种病理改变的总积分与模型组差异显著.结论 中药熏蒸疗法治疗类风湿性关节炎的机制可能与抑制炎性细胞浸润、纤维组织增生及滑膜细胞增生有关.     

防风色原酮提取物对大鼠胶原诱导性关节炎的影响

目的:研究防风色原酮提取物对大鼠胶原诱导性关节炎(collagen Ⅱ-induced arthritis,CIA)的防治作用及初步机制。方法:Ⅱ型胶原二次免疫诱导大鼠CIA模型,初次免疫前3d分别以0.21,0.42,0.84g·kg^-1量ig防风色原酮提取物,动态观察大鼠关节炎积分和发病率;免疫后35d取材,观察关节组织病理学和放射影像学变化;酶联免疫吸附法检测血清抗Ⅱ型胶原抗体IgG和IgM水平;放射免疫分析法检测血清中白细胞介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)含量。结果:防风色原酮提取物能降低大鼠关节炎积分和发病率,抑制血清中IgG和IgM含量,减轻关节滑膜、软骨和骨的组织病理学变化及X光片的骨破坏程度,降低血清IL-1β,TNF-α和IL-6水平。结论:防风色原酮提取物能控制大鼠CIA病情的发生发展,其机制可能与对促炎细胞因子的有效抑制有关。     

人软骨糖蛋白-39对CIA大鼠淋巴细胞外增殖作用的研究

目的 观察人软骨糖蛋白-39( HCgp39)对胶原诱导型关节炎(CIA)大鼠淋巴细胞激活的影响,探讨其在类风湿关节炎(RA)发病中的作用.方法 建立CIA大鼠模型,分别在建模1、2、3、4、5、6、7、8周后,分离、培养大鼠脾淋巴细胞,CCK-8法检测HCgp39对淋巴细胞增殖的影响,ELISA法检测血浆中抗HCgp39抗体及COMP的分泌水平,分析指标之间相关性.结果 建模2周后,HCgp39抗原特异性T细胞与对照组比较出现明显增殖反应(P均＜0.01),与建模时间、抗HCgp39抗体水平显著正相关,与血管翳和滑膜炎症评分显著负相关.各组抗HCgp39抗体水平和COMP水平较对照组显著增高(P均＜0.01),且抗HCgp39抗体水平与建模时间及COMP呈显著正相关.结论 HCgp39可刺激CIA大鼠脾淋巴细胞的体外异常增殖,初步提示HCgp39抗原短肽在CIA早期及后续的发病过程中可能起着一定作用.     

T cell receptor signaling induced by an analog peptide of type II collagen requires activation of Syk

We have previously described an analog peptide of type II collagen (CII) that can suppress collagen-induced arthritis (CIA). This analog peptide represents CII_245–270, the immunodominant epitope of CII, but with substitutions at 260, 261, and 263 — CII_245–270 (A₂₆₀, B₂₆₁, and N₂₆₃) (A9). To elucidate the mechanisms responsible for suppression, we used mice transgenic for a collagen-specific T cell receptor (TCR). When we found that APCs pulsed with A9 failed to induce T cell phosphorylation of TCR-ζ and ZAP-70, we explored alternative signaling pathways. We determined that A9 instead induced phosphorylation of spleen tyrosine kinase (Syk). The importance of Syk was confirmed by the use of chemical Syk inhibitors, which blocked both cytokine secretion and activation of GATA-3 mediated by peptide A9. In summary, T cells use an alternative pathway in response to A9 that involves Syk. This novel T cell pathway may represent an important means for altering T cell phenotypes.