胰腺癌患者增殖细胞核抗原评分和Bcl-2、P53蛋白表达

胰腺癌患者增殖细胞核抗原评分和Ｂｃｌ－２、Ｐ５３蛋白表达杨竹林李永国庄赞根钟德王午吕方作者应用ＡＢＣ免疫组织化学法，研究了胰腺癌组织中增殖细胞核抗原（Ｐｒｏｌｉｆｅｒａｔｉｎｇｃｅｌｎｕｃｌｅａｒａｎｔｉ－ｇｅｎ，ＰＣＮＡ）评分和Ｂｃｌ－２、Ｐ５３基...     

肝癌组织中HOXA9 mRNA的表达及其临床意义

目的研究肝癌病例癌组织、癌旁组织及非癌肝组织中HOXA9 mRNA的表达及其临床意义.方法标本经10%中性福尔马林固定后常规制作石蜡包埋切片,HOXA9 mRNA行原位杂交检测.结果临床分析发现术后血清AFP值、AFU值、Glo值、PAB值及SHCSP阳性率均有明显减少.癌组织HOXA9mRNA阳性表达评分(1.94±1.69)明显低于非癌肝组织(3.17±1.70),两者有显著差异;癌旁组织HOXA9mRNA阳性表达评分(2.54±1.20)与非癌肝组织及癌组织无显著差异.癌组织、癌旁组织及非癌肝组织三者HOXA9mRNA阳性表达率(54.84%、76.92%、83.33%)无显著差异.除HCC中HOXA9RNA阳性表达评分(1.81±1.78)显著低于MHC(2.75±0.50)外,HOXA9mRNA表达阳性率及阳性评分与癌分化程度、AFP值、SHCSP是否阳性、AFU值、肝硬化有无、转移情况、有无癌栓、大体形态及肿块最大径等临床病理特征均无明显关系.结论本研究结果显示HOXA9mRNA可能与肝癌的发生有密切关系.临床上检测HOXA9mRNA的表达情况对肝癌的早期发现和早期诊断可能有较重要的临床意义,值得深入研究.     

慢性肝病、原发性肝癌病人血清、肝组织纳米细菌的检测

目的 研究慢性肝病和原发性肝癌病人血清、组织中纳米细菌（NB）感染，为其发生机制提供新的认识。方法 55例慢性肝病（25例慢性乙肝和30例肝炎后肝硬化）、43例肝癌病人和336例健康人血清采用ELISA、免疫组化和钙染色；53例慢性肝病（28例慢性乙肝和25例肝硬化）、43例肝癌和15例对照肝组织行免疫组化染色，部分阳性组织透射电镜观察。结果 （1）慢性肝病、肝癌病人和健康人血清ELISA检测阳性率分别为20．0％、9．3％和8．0％，慢性肝病NB感染率高于健康人（P〈0．05）。免疫组化染色感染率分别为14．5％、4．7％和5．7％（P〈0．05）。钙染色分别为7．3％、4．7％和6．5％（P〉0．05）。（2）慢性肝病、癌及癌旁、对照组免疫组化阳性率分别为：20．8％、16．3％、14．0％和0％（P〉0．05）。部分肝癌和癌旁组织透射电镜观察发现纳米细菌样微生物结构（3／5）。结论 部分慢性肝病、肝癌病人血清、组织中存在纳米细菌感染，慢性肝病血清中纳米细菌感染率高于健康人。     

胆囊良恶性病变中胆囊星形细胞标记物及其调控因子的表达研究

目的 探讨胆囊腺癌、癌旁组织和慢性胆囊炎组织中GSCs和其调控因子TGF β1mRNA和CTGFmRNA表达水平及其临床病理意义。方法 108例胆囊腺癌、46例癌旁组织和35例慢性胆囊炎手术切除标本常规石蜡包埋切片，GSCs染色方法为平滑肌肌动蛋白（α SMA）单抗EnVisiomTM免疫组化法，TGF β1mRNA和CTGFmRNA染色方法为原位杂交法。结果 胆囊腺癌α SMA，TGF β1mRNA，CTGFmRNA表达阳性率及其评分明显高于癌旁组织和慢性胆囊炎（P<0.05或P<0.01）；但腺瘤癌变、肿块最大径<2cm、无淋巴结转移和未侵犯周围组织者的α SMA，TGF β1mRNA，CTGFmRNA表达阳性率及其评分明显高于低分化腺癌、肿块最大径≥2cm、淋巴结转移和侵犯周围组织器官者（P<0.05或P<0.01）；胆囊腺癌中α SMA，TGF β1mRNA，CTGFmRNA表达评分之间均呈高度密切正相关（α SMA vs TGF β1mRNA, r=0.82; α SMA vs CTGFmRNA r=0.75; TGF β1mRNA vs CTGFmRNA, r=0.78）结论 α SMA，TGF β1mRNA，CTGFmRNA均为反映胆囊腺癌进展、生物学行为及预后的重要生物学标记物，TGF β1和CTGF在活化GSCs方面可能具有重要调控作用。     

胃良性和恶性病变组织中β-半乳糖凝集素3和接骨木凝集素的表达及其临床病理意义

Objective To study the expressive levels of galectin-3(gal-3) and sambucus nigra agglutinin (SNA) and their clinicopathological significance in the benign and malignant lesions of stomach. Methods EnVisonTM immunohistochemistry for assaying gal-3 expressive level and ABC cytochemistry for determining SNA expressive level were used in conventional paraffin-embedded sections from specimens of gastric cancer(n=49), peritumoral tissues(n=20), metastastic foci of lymph nodes(n=36), and different types of benign lesions(n=80). Results The positive rates of gal-3 and SNA were significantly higher in gastric cancer tissues than those in peritumoral tissues and different types of benign lesions (P<0.05, P<0.01). The positive cases of gal-3 and/or SNA in peritumoral tissues and benign lesions showed mild- to severe-atypical hyperplasia of mucous epithelial cells. No difference was found between the primary foci and metastatic foci in gal-3 and SNA expressions (P>0.05). The positive rates of gal-3 and SNA were significantly lower in histologic grade Ⅱ, infitrating depth T1,T2 and no-metastasis of regional lymph node than those in histologic grade Ⅲ, Ⅳ, infitrating depth T3,T4 and metastasis of lymph node in gastric cancer(P<0.05). The positive rates of gal-3 and SNA were higher in lymphnede metastatic site N1 and no-metastasis of distant organs than those in lymphnede metastatic site N2, N3 and metastasis of distant organs, but no significant difference was found (P>0.05). The consistence was found between the expression of gal-3 and SNA in gastric cancer tissues (χ2=6.59,P<0.05). Conclusions The expressive levels of gal-3 and SNA may be important molecular markers of lectins for reflecting the carcinogenesis, progression and biological behaviors in gastric cancer.     

胃良性和恶性病变组织中β-半乳糖凝集素3和接骨木凝集素的表达及其临床病理意义

Objective To study the expressive levels of galectin-3(gal-3) and sambucus nigra agglutinin (SNA) and their clinicopathological significance in the benign and malignant lesions of stomach. Methods EnVisonTM immunohistochemistry for assaying gal-3 expressive level and ABC cytochemistry for determining SNA expressive level were used in conventional paraffin-embedded sections from specimens of gastric cancer(n=49), peritumoral tissues(n=20), metastastic foci of lymph nodes(n=36), and different types of benign lesions(n=80). Results The positive rates of gal-3 and SNA were significantly higher in gastric cancer tissues than those in peritumoral tissues and different types of benign lesions (P<0.05, P<0.01). The positive cases of gal-3 and/or SNA in peritumoral tissues and benign lesions showed mild- to severe-atypical hyperplasia of mucous epithelial cells. No difference was found between the primary foci and metastatic foci in gal-3 and SNA expressions (P>0.05). The positive rates of gal-3 and SNA were significantly lower in histologic grade Ⅱ, infitrating depth T1,T2 and no-metastasis of regional lymph node than those in histologic grade Ⅲ, Ⅳ, infitrating depth T3,T4 and metastasis of lymph node in gastric cancer(P<0.05). The positive rates of gal-3 and SNA were higher in lymphnede metastatic site N1 and no-metastasis of distant organs than those in lymphnede metastatic site N2, N3 and metastasis of distant organs, but no significant difference was found (P>0.05). The consistence was found between the expression of gal-3 and SNA in gastric cancer tissues (χ2=6.59,P<0.05). Conclusions The expressive levels of gal-3 and SNA may be important molecular markers of lectins for reflecting the carcinogenesis, progression and biological behaviors in gastric cancer.     

γ—GT在膀胱癌中的表达及意义

γ-谷氨酰转肽酶(γ-GT)是一种含糖蛋白的膜结合酶,因它与癌转化关系密切而受到广泛重视.消化系统及乳腺有关肿痛的γ-GT酶组化观察均已有文献报道但膀胱癌γ-GT组化观察未见文献报道,本文应用酶组化方法研究正常膀胱粘膜、乳头状癌及膀胱癌组织中γ-GT的表达及其意义.材料与方法     

胰腺癌组织中P-糖蛋白、雌激素诱发蛋白表达的临床意义

胰腺癌化疗失败的主要原因之一是肿瘤细胞对化疗药物产生耐药性 ,多药耐药性是一种独特的广谱耐药现象。研究表明多药耐药基因 (MDR)表达产物P 糖蛋白 (P glycopro tein ,P gp)与多种临床常见肿瘤的多药耐药表型有关 ,是肿瘤细胞产生耐药性的主要机制之一。雌激素诱发蛋白 (pre senilin 2 ,pS2 )是从乳腺癌MCF 7细胞株中提取的一种雌激素诱发蛋白。在乳腺癌细胞内 ,pS2只有在雌激素控制下才能被转录。pS2表达研究对了解乳腺癌等恶性肿瘤生物学行为、激素依赖性及指导临床辅助治疗具有重要价值。本文研究胰腺癌组织中P gp和 pS2的表达…     

大鼠胰腺癌和非癌胰腺组织Skp2和p27表达研究

目的建立大鼠胰腺癌模型,研究大鼠胰腺癌和非癌胰腺组织中Skp2和p27表达及其意义。方法应用二甲基苯并蒽(DMBA)置入大鼠胰实质内(A组)及设立曲古霉素(TSA)干预组(B组),3~5个月内处死观察胰腺癌发生情况,应用SP免疫组化法研究胰腺癌和非癌胰腺组织中Skp2和p27表达。结果3~5个月内A组发癌率为48.7%(18/37),B组为33.3%(12/36),A组和B组各1例纤维肉瘤外,余均为胰腺导管癌;A组肿块最大径大于B组(P<0.05);非癌胰腺组织中A组导管上皮中至重度不典型增生者(52.6%)高于B组(33.3%),但差异无显著性(P>0.05);C组胰腺和A或B组鼠胰腺外主要脏器均无明显病变。A组和/或B组胰腺导管癌Skp2表达阳性率均明显高于A组和/或B组非癌胰腺组织(P<0.05);但胰腺导管癌p27表达阳性率明显低于非癌胰腺组织(P<0.01);Skp2阳性和p27阴性的非胰腺组织导管上皮均呈中至重度不典型增生;C组胰腺导管上皮Skp2均阴性表达和p27均阳性表达。结论DM-BA置入胰实质内可在短期获得较高的SD鼠胰腺癌发生率,TSA能抑制胰腺癌发生和生长。Skp2和p27表达水平与SD鼠胰腺癌发生发展有密切关系。     

胰腺癌组织中Kail和骨桥蛋白表达及其临床病理意义

目的 研究胰腺导管癌（PC）和慢性胰腺炎（CP）组织中Kail和骨桥蛋白（OPN）表达水平并探讨其临床病理意义及其相互关系。方法 51例PC和10例CP手术切除标本常规制作石蜡包埋切片，Kail和OPN染色方法均为ABC免疫组化法。结果 51例PC中Kail表达阳性率低于CP（P〈0.05）,OPN表达阳性率高于CP（P〈0.01）；高分化和未转移PC中Kail表达阳性率明显高于低分化和转移病例，且差异有统计学意义（P〈0.05）;低分化腺癌OPN表达阳性率与高分化腺癌比较差异有统计学意义（P〈0.05）;两者存在密切关系（P〈0.01）。结论 Kail和OPN表达可能是反映PC发生，发展及预后的重要生物学标记物。