我院人体组织生物样本库的建立和应用

Objective To explore issues of the human tissue biobank, ranging from its establishment, collection and preservation of samples, quality control, management and application. Methods Development of standardized operational procedures, collection of such samples as fresh frozen tissues from the surgery, paraffin-embedded tissues, whole blood, serum, plasma, and cerebrospinal fluid. Specimens were classified and made aliquots according to different requirements, and then stored at room temperature, -80℃ refrigerator or in liquid nitrogen. Microsoft Access database system was used in the management of these specimens. Results From September 2004 to September 2008, a total of 11 872 samples from patients with benign and malignant diseases were collected and preserved. Among them, 4360 tubes of fresh frozen tissue samples from 2500 cases were provided within and beyond the province. These samples were also applied to DNA, RNA, protein extraction and tissue microarray, and immunohistochemistry-related research. Conclusion Human tissue biobank is highly useful in sharing human tissue resources effectively, as it can provide high quality specimens from benign and malignant diseases and normal control. In addition, it plays a very important role in exploring pathogenesis, developing new technologies for early detection of disease and new therapeutic strategies.     

我院人体组织生物样本库的建立和应用

Objective To explore issues of the human tissue biobank, ranging from its establishment, collection and preservation of samples, quality control, management and application. Methods Development of standardized operational procedures, collection of such samples as fresh frozen tissues from the surgery, paraffin-embedded tissues, whole blood, serum, plasma, and cerebrospinal fluid. Specimens were classified and made aliquots according to different requirements, and then stored at room temperature, -80℃ refrigerator or in liquid nitrogen. Microsoft Access database system was used in the management of these specimens. Results From September 2004 to September 2008, a total of 11 872 samples from patients with benign and malignant diseases were collected and preserved. Among them, 4360 tubes of fresh frozen tissue samples from 2500 cases were provided within and beyond the province. These samples were also applied to DNA, RNA, protein extraction and tissue microarray, and immunohistochemistry-related research. Conclusion Human tissue biobank is highly useful in sharing human tissue resources effectively, as it can provide high quality specimens from benign and malignant diseases and normal control. In addition, it plays a very important role in exploring pathogenesis, developing new technologies for early detection of disease and new therapeutic strategies.     

石蜡组织芯片制备技术的改良

目的：探讨组织芯片制备方法以及相关技术改良,提高组织芯片制备的质量。方法：使用组织芯片空心蜡模一次性成型的模具,应用改良的组织芯片制备方法以及二次石蜡包埋技术,制作食管癌肿瘤组织芯片,常规病理石蜡切片制备组织芯片,观察实验结果。结果：组织芯片空心蜡模制备成功率达100%。所制备的组织芯片石蜡块内组织芯切片平面整齐,无组织芯倒浮现象。组织芯片切片中组织芯片微组织片阵列整齐,无微组织片皱褶以及微组织片脱片现象。组织芯片常规HE染色后,经病理诊断医生阅片,并与各组织芯原供体石蜡标本常规病理HE染色片进行对照观察,病理诊断结果一致。结论：改良的石蜡组织芯片制备技术简单易学,操作流程容易掌握控制,提高了组织芯片制作的质量,有利于组织芯片技术的普及和推广应用。     

USP14在结直肠癌中的表达及临床意义

目的 探讨USP14在结直肠癌中的表达及其临床意义。 方法 选取225例结直肠癌患者肿瘤组织及对应的远端正常组织,采用免疫组化法检测USP14表达情况,并分析其与临床病理参数间的关系。 结果 结直肠癌组织中USP14表达显著高于正常组织(χ²=11.62,P<0.05),USP14表达情况与患者性别(χ²=0.205,P=0.651)、年龄(χ²=1.274,P=0.259)、远处转移(χ²=0.739,P=0.390)、TNM分期(χ²=3.082,P=0.079)无显著相关。生存分析发现,USP14阳性表达患者的平均生存时间(63.78个月,95% CI:57.37~70.19)明显短于USP14阴性表达患者(83.09个月,95% CI:75.40~90.78,P=0.003)。COX比例风险回归分析表明,USP14表达(HR=2.213,P=0.002)和TNM分期(HR=3.237,P=0.000)可作为结直肠癌患者的独立预后因素。 结论 USP14在结直肠癌发展中发挥重要作用,USP14表达是结直肠癌患者的独立预后因素。     

我院人体组织生物样本库的建立和应用

目的 探讨人体组织生物样本库建设,标本收集、保存、质最控制、管理及应用等有关问题.方法 制定标准化操作流程,收取配套新鲜冷冻组织、石蜡包埋组织、全血、血清、血浆、脑脊液等标本,按不同要求处理分装,常规、液氮或-80℃超低温冰箱冷冻保存,应用数据库管理系统进行信息化管理.结果 从2004年9月至2008年9月共收集各类疾病标本504个病种,11872例33137管份,至今已向省内外提供新鲜冷冻组织标本2500例4360管,应用于DNA、RNA、蛋白提取及组织芯片、免疫组织化学等的相关性研究.结论 人体组织生物样本库的建立,有利于有效利用不可再生的人体组织标本资源,随时提供符合要求的良恶性疾病标本和正常对照标本;有利于探讨疾病的致病机制、开发疾病早期榆测新技术和新的治疗策略.     

高尔基体糖蛋白-73在非小细胞肺癌中的表达及意义

目的 研究非小细胞肺癌(non small cell lung cancer,NSCLC)和正常肺组织中高尔基体糖蛋白-73(Golgi protein 73,GP73)的表达差异,探讨GP73表达与NSCLC发生、发展及其预后的相关性。方法 免疫组织化学法检测90例NSCLC组织和19例肺正常组织GP73的表达,结合临床病理因素分析其与NSCLC预后的相关性。ROC曲线分析GP73用于肺腺癌和鳞癌鉴别诊断的价值。结果 NSCLC中GP73表达较正常肺组织高,两者比较差异有统计学意义(P<0.05),GP73表达与组织学类型及临床病理分期显著相关,而与NSCLC肿瘤分化程度、性别、年龄及术后生存期不相关。ROC曲线下面积为0.710(95%可信区间为0.590~0.831,P<0.05)。结论 GP73在NSCLC组织中高表达,且与病理类型及临床分期显著相关,GP73对肺腺癌和鳞癌的鉴别诊断具有潜在价值。