幽门螺杆菌活载体疫苗的研究进展

幽门螺杆菌（Helicobacter pylori,HP）被公认为是慢性活动性胃炎、消化性溃疡等疾病发生的主要病因,并与胃腺癌、胃淋巴瘤的发生密切相关,是一级致癌因子[1]。虽然HP确切的致病机制尚未完全阐明,但HP必须首先定植于胃粘膜,然后再进一步侵入宿主防御系统,由其毒素的直接作用和诱导的炎症反应等间接作用损伤组织而致病。流行病学调查研究显示，我国幽门螺旋杆菌感染率约为40％～60％，儿童也有相当高的感染率。     

原肌球蛋白4通过慢病毒重组体技术应用于脊髓损伤后的作用及 机制研究：随机对照实验方案

BACKGROUND: Tropomyosin 4 level has been found to be an increase in the spinal cord based on the 2-DE/MALDI-TOF/MS method. However, there is little report about the relationship between tropomyosin 4 and pathogenesis and progress of spinal cord injuries. METHODS/DESIGN:Randomized controlled trial: rat models of complete spinal cord transection were made and expression levels of tropomyosin 4 at 3-28 days after modeling were determined by two-dimensional electrophoresis, animo acid serie analysis, quantitative PCR and western blot. Experiment for exporing the genetic mechanism: effects of tropomyosin 4 scilencing by lentivirus recomnination technology on the dendrite length of spinal cord neurons in vitro were observed, and its effects on the neurological function of rats after complete spinal cord transaction were assessed through Basso, Beattie, and Bresnahan scoring. DISCUSSION: This study will be powered to provide a novel and effective treatment strategy for neurological function recovery after spinal cord transection based on the lentivirus recomnination carrying tropomyosin 4, as well as optimistic future for clinical gene treatment of complete spinal cord transaction through figuring out the underlying mechanism. ETHICAL APPROVAL: This study was approved by the Ethics Committee of Kunming Medical University, China. The surgical operation and postoperative care of rats were in line with the rules of Chinese Experimental Animal Protection and Ethics Committee, and the guideline of the National Institutes of Health 中国组织工程研究杂志出版内容重点：组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松;组织工程     

三七总黄酮抗糖皮质激素诱发骨质疏松的效应

  目的  研究三七总黄酮拮抗糖皮质激素诱发骨质疏松的效应及可能的机制。  方法  将50只体重约170 g的SPF级雌性SD大鼠随机分为五组（10只/组），即正常对照组、模型组（激素组）和三七总黄酮高、中、低剂量治疗组（激素 + 用药组）。模型组和三七总黄酮治疗组采用地塞米松（2.5 mg/kg）肌肉注射，每周2次；同时三个治疗组分别给予三七总黄酮275 mg/（kg·d）、137.5 mg/（kg·d）、68.75 mg/（kg·d）灌胃治疗，每天1次。实验期9周。9周后测定各组大鼠股骨骨密度、反映骨生物力学性能的指标及血清生化指标。  结果  与正常组比较，模型组大鼠股骨骨密度（BMD）、骨生物力学性能、血清钙水平明显降低，血清磷水平及血清碱性磷酸酶活性明显升高（P < 0.05）；与模型组比较，三七总黄酮高、中剂量治疗组大鼠股骨BMD、骨生物力学性能、血清钙水平明显增高，血清磷水平及血清碱性磷酸酶活性明显降低（P < 0.05）。  结论  三七总黄酮可提高糖皮质激素性骨质疏松大鼠骨密度，改善其骨生物力学性能，对糖皮质激素诱发的骨质疏松具有拮抗作用，作用机制与其抑制骨吸收及糖皮质激素诱导的钙磷代谢失衡有关。     

培养福氏志贺菌感染大鼠回肠组织病理学的特点

BACKGROUND:Bacillary dysentery is one of the most common enteric infectious diseases in summer and autumn that is mainly caused by Shigella. OBJECTIVE: To analyze the pathological features of the rat ileum after Shigella flexneri infection. METHODS: Totally 30 male Sprague-Dawley rats were enrolled and equally randomized into three groups. Rats in non-streptomycin and streptomycin groups were firstly treated with normal saline and 50 g/L streptomycin sulphate via gastric lavage, and then given gastric lavage of 9×108 CFU/mL Shigella flexneri. Rats were given no any treatment as control group. 14 days later, morphological changes of the rat ileum were observed through hematoxylin-eosin staining. RESULTS AND CONCLUSION: (1) After Shigella flexneri infection, the rat distal ileum and rectum were characterized by mucosal edema and hemorrhage, neutrophilic granulocyte exudation, pseudomembrane formation and destruction of goblet cells with hemorrhage. There were obvious fibrin exudation on the necrotic mucosal surface that constituted the pseudomembrane with necrotic debris, leukocytes and erythrocytes. After mucosa defulvium, non-uniform and superficial ulcers were mainly visible at the mucosa. (2) The occurrence time of positive systems, temperature and body mass before and after Shigella flexneri infection showed no significant differences among rats (P > 0.05). (3) These results suggest that the rat distal ileum and rectum after Shigella flexneri infection present the characteristic morphological futures of bacillary dysentery; therefore, it can be used as the animal model to undergo in-depth research about the etiology, pathogenesis and treatment of bacillary dysentery. 中国组织工程研究杂志出版内容重点：肾移植;肝移植;移植;心脏移植;组织移植;皮肤移植;皮瓣移植;血管移植;器官移植;组织工程     

三七皂苷Rg1对慢性应激大鼠脑组织神经颗粒素表达的影响

［摘要］目的　探讨三七皂苷Rg1对慢性应激模型大鼠皮层、海马神经颗粒素（neurogranin,Ng）表达的影响．方法　成年雄性SD大鼠36只,随机分为对照组（CON）、模型组（CUS）和治疗组（CUS-G）,采用慢性不可预见性应激方法建立慢性应激动物模型,运用Morris水迷宫实验对大鼠进行学习记忆力测试,QPCR、Western blot分别检测皮层、海马Ng mRNA及Ng蛋白含量．结果　水迷宫实验显示慢性应激后动物学习记忆能力显著降低,而治疗组大鼠逃避潜伏期明显降低（P＜0.0 5）;应激6周后,慢性应激大鼠皮层、海马Ng mRNA水平皆与模型大鼠有差异（P＜0.05、P＜0.01）,治疗组大鼠皮层、海马Ng mRNA水平皆与模型大鼠有差异（P＜0.01、P＜0.05）;慢性应激大鼠皮层、海马Ng含量水平与模型大鼠有差异（P＜0.05、P＜0.01）,治疗大鼠皮层、海马Ng含量亦与模型大鼠有差异（P＜0.01、P＜0.05）．结论　慢性不可预见性应激导致大鼠皮层、海马Ng mRNA水平、NG 蛋白含量显著下降,造成大鼠学习记忆功能减退,三七皂苷Rg1可上调Ng mRNA水平、NG蛋白含量表达,且对慢性应激所致外显行为有积极调节作用．