CD3+木瓜提取物对ApoE基因缺陷小鼠血脂及炎性相关因素的影响

目的观察木瓜提取物对ApoE基因缺陷小鼠血脂水平、主动脉窦斑块中巨噬细胞活化及肿瘤坏死因子(TNF-α)水平的影响。方法30只雄性ApoE基因缺陷小鼠随机分为3组:对照组、低剂量组和高剂量组,低剂量组和高剂量组分别在基础饲料上添加5%和10%的木瓜提取物喷雾干燥颗粒,饲养16周后经小鼠眼眶静脉取血后处死动物,酶法测定血脂水平、采用免疫组化分析主动脉窦斑块中TNF-α的表达水平和采用免疫荧光反映斑块CD68阳性表达水平与巨噬细胞阳性表达率。结果低剂量组和高剂量组的血清TC和LDL-C显著低于对照组(P<0.05),木瓜提取物能显著下调斑块中TNF-α的表达水平以及减少巨噬细胞的阳性率。结论木瓜提取物降低了血清脂蛋白胆固醇水平(TC和LDL-C),减少了炎性因子TNF-α表达以及减弱巨噬细胞参与程度,预示其可能具有减缓动脉斑块发生发展的抗动脉粥样硬化作用。     

TNF-α基因转染对胃癌细胞系的生长抑制效应

名誉主编Ill,llorarrbotoy-n-che吴盂超WuMe叱咖肋（fecorlrlMilitalyMhMUniversty,戳皿吟凶2（xM.--）学术顾问WAdfor巴德年BaDe。（hnesepofMhcaleiences,Betinglop30）刘新垣iulmp（孔皿吟dInstaofBbo,Cllllleseqofdences,砌吧饲2（XXB）吴妄Wuin（CancerInstitUte,ChineseA脚加阳OfMeicalde。,阿吨l（XXll）汤＄1JffiTpZhaoou（aMhcalUmversity,枷呷饲ZffX）32）主编itoY—n—cief张友会Zllazlzyouhni（CancelnstitUte,ClllxleseAcAsyofMwhedAs。,Betwl（XXll）副主编A田叨h扣hoot.勿一咖姓崔正言ClZhangan（Depe…     

痹症停合剂对大鼠佐剂性关节炎的防治作用及其机制研究

痹症停合剂是由雷公藤,赤芍,防风等10余味中药组成.临床主要用于治疗类风湿关节炎.本文研究痹症停合剂对大鼠佐剂性关节炎的防治,治疗作用,并观察其对大鼠腹腔巨噬细胞产生IL-1,TNF-a的影响,用以探讨其作用机制.为临床应用提供药理学依据.     

Immune stimulatory effects of Loranthi ramulus on macrophages through the increase of NO and TNF-α

The activation of macrophages by microorganisms plays an important role in host defense and immunopathology. Loranthi ramulus (LR) is commonly used as a traditional drug and health food in Korea. Here, we investigated the regulatory effects of LR on macrophage-mediated immune responses. Treatment of macrophages with LR resulted in the enhanced cell-surface expression of CD80, CD86 and major histocompatibility complex (MHC) class II, as well as the enhanced production of nitric oxide (NO) and tumor necrosis factor (TNF)-α, and also iNOS and TNF-α mRNA expression. These alterations of LR-treated cells were associated with the activation of NF-κB and mitogen-activated protein kinases (MAPKs). LR increased the phosphorylation of MAPKs (JNK, ERK1/2, p38 MAPK) and the activation of NF-κB in Raw 264.7 cells. These results suggest that LR has increased NO and TNF-α production through phosphorylation of all three MAPKs following IκBα degradation and NF-κB activation. In conclusion, our results demonstrate that LR can effectively promote the activation of macrophages, suggesting that LR may possess the potential to regulate immune responses.     

Does choice of antiretroviral drugs matter for inflammation?

Introduction: The massive implementation of combination antiretroviral therapy (cART) has forever changed the landscape of HIV infection. This unprecedented success has turned HIV infection into a manageable chronic disease. The increased survival of people living with HIV is, however, shadowed by a high burden of aging-related comorbidities. The pathogenic basis underlying this excess of co-morbid conditions is most likely a persistent inflammatory and immune activation state, despite an optimal control of HIV replication, which in turn has largely been attributed to bacterial or bacterial products translocation from the gut.

Area covered: This review is focused on the relationship between cART and the chronic inflammatory and immune activation status in otherwise virologically well-controlled people living with HIV (PLWH). Particular focus will be placed on the differences, if any, between distinct cART modalities, with emphasis on less-drug cART regimens, and especially on dual therapies.

Expert opinion: Research to address the increased inflammatory and immune activation status of cART-treated, HIV-infected patients, should focus on adjuvant means of therapy, rather than on the cART regime itself. With current antiretrovirals, no difference between dual and triple regimens has been demonstrated, provided that virological and immunological outcomes be non-inferior.     

冠心病与牙周病患者IL-6、TNF-a、PGE2水平相关性研究

目的：探讨冠心病与牙周病患者牙龈沟液中IL-6、TNF-a、PGE2水平变化的相关性。方法：临床选择60~74岁的120例患者,分为3组,分别应用放射免疫法检测龈沟液中IL-6、TNF-a、PGE2含量。结果：与健康对照组相比,冠心病伴牙周病组及单纯牙周病组中,龈沟液中IL-6、TNF-a、PGE2含量均明显升高（P〈0.05）;其中冠心病伴牙周病组龈沟液中IL-6、TNF-a、PGE2含量高于单纯牙周病组（P〈0.05）。结论：冠心病与牙周炎存在一定的相关性,伴有冠心病的牙周炎患者龈沟液内IL-6、TNF-a、PGE2水平明显升高。     

醒脑静注射液辅助治疗化脓性脑膜炎患儿观察

目的：观察醒脑静注射液联合头孢曲松治疗化脓性脑膜炎患儿的疗效。方法：100例化脓性脑膜炎患儿随机分为对照组50例和观察组50例,对照组予常规治疗;观察组在对照组基础上加用醒脑静注射液0.4-0.6ml·kg-1·d-1。两组疗程均为3 d。比较两组体温正常、外周血及脑脊液WBC计数正常时间与住院时间,以及两组患儿治疗前后血及脑脊液中肿瘤坏死因子-α（TNF-α）、C反应蛋白（CRP）水平变化,评价两组临床疗效,比较并发症及随访6个月后的后遗症发生情况。结果：与对照组相比,观察组体温正常时间和脑脊液WBC计数正常时间均明显缩短（P〈0.05）,血清及脑脊液TNF-α含量下降更显著（P〈0.05）,观察组临床总有效率明显高于对照组（P〈0.05）。其余指标两组比较差异无统计学意义（P〉0.05）。结论：醒脑静注射液辅助治疗化脓性脑膜炎能有效改善患儿症状,纠正炎症因子异常,临床疗效高,值得临床推广。     

阿魏酸钠对佐剂关节炎大鼠血清中瘦素和肿瘤坏死因子-a表达水平的影响

类风湿关节炎(RA)是一种以侵蚀性关节炎为主要表现的全身性自身免疫病,以女性多发,以双手和腕关节等小关节受累为主的对称性、持续性多关节炎。病理表现为关节滑膜的慢性炎症、血管翳的形成,并出现关节软骨和骨破坏,最终可导致关节畸形和功能丧失。阿魏酸钠(Sodium Ferulate,SF)是我国自行研发的一类新的非肽类内皮素受体拮抗剂,普遍存在于当归、川芎、蜂胶、酸枣仁等中药中,可拮抗内皮     

亚硒酸钠、硫酸镁对石英致细胞因子释放和羟脯氨酸合成影响的体外研究

[目的]观察亚硒酸钠(Na_2SeO_3)、硫酸镁(MgSO_4)对二氧化硅(石英)致巨噬细胞(RAW264.7)释放肿瘤坏死因子(TNF-a)、白介素-1(IL-1)的单独拮抗作用以及对人胚肺成纤维细胞羟脯氨酸(Hyp)合成水平变化的单独以及联合拮抗作用。[方法]对数生长期的RAW264.7细胞,调整浓度为2×10~5个/ml。在染200mg/L石英同时单独加入不同浓度Na_2SeO_3、MgSO_4溶液,24h后测定培养液中TNF和IL-1水平,并取培养液与人胚肺成纤维细胞共同培养,48h后测定人胚肺成纤维细胞培养液中Hyp含量。另外,在染石英同时联合加入不同浓度的Na_2SeO_3和MgSO_4溶液,培养24h后取培养液与人胚肺成纤维细胞共同培养,48h后测定其中的Hyp含量。[结果]①与石英组比,Na_2SeO_3对RAW264.7细胞释放TNF-α无明显影响,MgSO_4可以显著降低TNF-α的活性(P<0.05),其中以1.0μmol/L MgSO_4的效果更为明显。②与石英组比,一定剂量的Na_2SeO_3、MgSO_4可以降低IL-1的活性,其中以0.5μmol/L Na_2SeO_3和2.0μmol/L MgSO_4的效果最好(P<0.05)。③与石英组比,一定剂量的Na_2SeO_3、MgSO_4可以显著降低人胚肺成纤维细胞Hyp合成水平(P<0.05),其中以2.0μmol/L Na_2SeO_3和2.0μmol/L MgSO_4的效果最明显。④与石英组比,一定剂量的Na_2SeO_3与MgSO_4联合可以降低人胚肺成纤维细胞Hyp合成水平,其中以2.0μmol/L Na_2SeO_3和2.0μmol/L MgSO_4联合作用效果最明显(P<0.05)。[结论]一定剂量的MgSO_4对石英致巨噬细胞释放TNF-α具有拮抗作用;一定剂量的Na_2SeO_3和MgSO_4对石英致巨噬细胞释放IL-1具有单独拮抗作用,对人胚肺成纤维细胞Hyp合成水平具有单独及联合拮抗作用。     

IL-13和TNF-a在支气管哮喘及慢性阻塞性疾病(COPD)中的临床研究

目的本研究旨在探讨血清细胞因子IL-13、TNF-a在支气管哮喘、慢性阻塞性肺疾病(COPD)患者中的浓度变化,探讨IL-13、TNF-a与哮喘、慢性阻塞性肺疾病(COPD)的发病机理以及诊疗中意义。方法40例支气管哮喘C Asthma),40例慢性阻塞性肺疾病(COPD)患者及20例健康对照者,入院时进行肺功能检杳,同时抽取静脉血,用酶联免疫吸附试验(ELISA)测定血清中的细胞因子IL-13(白细胞介素13),TNF-a(肿瘤坏死因子)的浓度。结论支气管哮喘及COPD急性发作期患者的血清IL-13、TNF-α的浓度明显高于健康对照组(P<0.01)。提示IL-13、TNF-α细胞因子参与支气管哮喘及COPD发病的机制。