部队训练中暑72例临床分析

目的总结分析治疗部队训练中暑的临床经验。方法对72例中暑病人的治疗进行回顾分析。其中先兆中暑27例，轻度中暑30例，重度中暑15例。结果72例中暑患者中，痊愈69例（占95．8％），死亡3例（占4．2％）。结论对中暑病人及时正确地采取迅速有效的降温、大量补液等综合治疗措施，可以取得较好的疗效。     

锌修饰硅酸钙陶瓷涂层与骨整合

BACKGROUND: Zinc-modified calcium silicate (CaSiO3) bioceramics coating on the titanium surface prepared in preliminary experiments has good chemical stability and antibacterial property. OBJECTIVE: To observe the effects of zinc-modified CaSiO3 bioceramics coating on osteointegration. METHODS: MC3T3-E1 cells were respectively cultured on the titanium with zinc-modified CaSiO3 bioceramics coating (experiment group), titanium with CaSiO3 bioceramics coating (control group) and pure titanium (blank control group). Then, cell adhesion, proliferation, calcification rate and the expression of type I collagen and osteocalcin were detected. The implant materials mentioned above were respectively inserted into the femurs of New Zealand white rabbits, and after 1.5 months, the osteoproliferation and osteointegration between the implants and the host were tested. RESULTS AND CONCLUSION: In vitro experiment: The number of adhesive cells at 12 hours after co-culture was significantly increased in the experimental group compared with the control group and blank control group (P < 0.05). At 14 days after co-culture, cell proliferation ability and ability of calcium nodule formation in the experiment group were significantly better than those in the other groups (P < 0.05). At 21 days after co-culture, there was no significant difference in the expression of type I collagen, but the expression of osteocalcin in the experiment group was higher than that in the control group and blank control group (P < 0.05). In vivo experiment: In the experiment group, a large amount of bone substances were detected, the coating materials directly contacted with the bone interface, new bone tissues and little fibrous tissues were observed at the interface. In contrast, there was a small amount of bone hyperplasia in the control group and almost no bone hyperplase in the blank control group. Moreover, a small part of the implant directly contacted with the bone interface and the most part was separated from bone trabeculae by fibrous tissues. These findings indicate that zinc-modified CaSiO3 bioceramics coating can enhance the ability of osteointegration between titanium implants and the host.      

水平振摇在骨髓间充质干细胞提纯中的作用

目的研究水平振摇对全骨髓贴壁分离大鼠骨髓间充质干细胞在细胞数量、纯度、凋亡及远期增殖及分化能力的影响,探索更为简单、高效的间充质干细胞提纯方法。方法在全骨髓贴壁法的基础上,使用水平摇床,以180 r/min、4 h为干预条件,水平振摇后计算贴壁细胞总数量;使用流式细胞仪测定贴壁细胞纯度及凋亡情况;使用MTT法测定振摇后贴壁细胞24 h的增殖情况;对分离纯化后细胞使用定向诱导培养基进行诱导分化。结果经振摇处理后,获得的细胞总量有所下降,细胞纯度显著提升,凋亡略有增加,增殖情况变化不大,具有向成骨细胞和脂肪细胞分化的能力。结论水平振摇法可显著提高全骨髓贴壁法的提纯效率,具有推广价值。     

不同年龄大鼠终板软骨细胞抗氧化能力研究

目的 :通过研究不同年龄大鼠的终板软骨细胞氧化应激的差异,探讨衰老对终板软骨细胞抗氧化能力的影响。方法:原代培养获取SD大鼠2个月、18个月来源的终板软骨细胞鉴定后,使用第三代细胞进行实验。实验分为两组,A组为2个月大鼠来源的终板软骨细胞,B组为18个月大鼠来源的终板软骨细胞。待A、B两组细胞贴壁后,通过β-半乳糖脱氢酶染色和RT-PCR对端粒酶长度进行检测,比较两组细胞衰老的差异;通过总抗氧化能力试剂盒检测两组细胞总抗氧化能力的不同。以浓度为200umol/L过氧化氢对细胞进行2.5h的处理,RT-PCR法检测处理前后两组细胞Ⅱ型胶原、蛋白聚糖、caspase-3及B淋巴细胞瘤-2(B-cell lymphoma-2,bcl-2)的m RNA表达情况,使用流式细胞仪检测两组细胞的凋亡情况。对Ⅱ型胶原、蛋白聚糖、caspase-3及bcl-2的相对表达量进行组间t检验,P0.05为差异有统计学意义。结果 :未使用过氧化氢处理时,A、B两组终板软骨细胞的在β-半乳糖脱氢酶染色、Ⅱ型胶原、蛋白聚糖表达及凋亡率无明显差异(P0.05),而B组在端粒酶的相对长度(T/S=10±2)及总抗氧化能力(0.72±0.18)较A组(T/S=135±5,1)显著降低(P0.05)。在相同条件的过氧化氢刺激后,B组终板软骨细胞的Ⅱ型胶原(0.60±0.16)、蛋白聚糖(0.75±0.22)表达明显低于A组细胞(P0.05),而凋亡率显著高于A组细胞(P0.05)。结论 :不同年龄大鼠终板软骨细胞衰老及抗氧化能力存在差异,衰老越明显的终板软骨细胞,总抗氧化能力越低,抵抗过氧化损伤的能力越差。     

应用人工智能识别超广角眼底照相多病种的初步研究

目的:构建一个小样本超广角眼底照相（UWFI）多疾病分类人工智能模型,初步探究人工智能对UWFI多病种分类任务的能力。方法:回顾性研究。2016年至2021年于武汉大学人民医院眼科就诊并行UWFI检查的1 123例患者的1 608张图像用于UWFI多疾病分类人工智能模型构建。其中,糖尿病视网膜病变（DR）、视网膜静脉阻...     

高浓度骨桥蛋白诱导双足直立雌性小鼠脊柱侧凸模型

BACKGROUND: In recent years, the relevant research confirmed that increased levels of osteopontin may lead to the development of adolescent idiopathic scoliosis. The establishment of appropriate animal models of adolescent idiopathic scoliosis is of great significance in the research and treatment of such diseases. OBJECTIVE: To observe the occurrence of scoliosis of the bipedal female mice induced by high concentration of osteopontin, and provide evidence for establishing ideal drug models of adolescent idiopathic scoliosis. METHODS: C3H/HeJ mice of the same age were selected and randomly assigned to high-concentration osteopontin female mice (n=20) and male mice (n=20). This study also set control male mice group (n=20) and control female mice group (n=20). Mice aged 3 weeks from four groups were subjected to upper limb amputation and rat tail resection to establish bipedal mouse models. After surgery, a special cage was used to induce the upright state. High-concentration osteopontin female mice and male mice were daily intraperitoneally injected with high-concentration osteopontin (40 μg/kg). Mice in the control group were intraperitoneally injected with physiological saline. At 3 months after induction, animal spine X-ray examination was conducted to measure Cobb angle. If Cobb angle was greater than 10°, a successful induction was set. The incidence and severity of scoliosis were evaluated. RESULTS AND CONCLUSION: (1) In the high-concentration osteopontin female mice group, scoliosis was observed in 18 mice. Cobb angle was 16°-38°, averagely (25.8±6.7)°. In the high-concentration osteopontin male mice group, scoliosis was observed in 16 mice. Cobb angle was 11°-34°, averagely (20.9±6.8)°. In the control male mice group, scoliosis was observed in 8 mice. Cobb angle was 12°-21°, averagely (15.6±3.1)°. In the control female mice group, scoliosis was observed in 9 mice. Cobb angle was 11°-24°, averagely (17.1±4.5)°. (2) The incidence of scoliosis was significantly higher in the high-concentration osteopontin female and male mice groups than in the control female and male mice groups (P < 0.05). No significant difference in the incidence of scoliosis was detected between high-concentration osteopontin female and male mice groups (P > 0.05). However, curve magnitude was significantly severer in the high-concentration osteopontin female mice group than in the high-concentration osteopontin male mice group (P < 0.05). (3) Results suggested that the scoliosis incidence was higher and scoliosis was severer in high-concentration osteopontin-induced bipedal mice, which was more closed to human. 中国组织工程研究杂志出版内容重点：肾移植;肝移植;移植;心脏移植;组织移植;皮肤移植;皮瓣移植;血管移植;器官移植;组织工程     

锌修饰硅酸钙生物陶瓷涂层的体外生物相容性研究

目的 研究锌修饰硅酸钙生物陶瓷涂层的体外生物相容性,为该涂层临床应用的可行性提供实验依据。方法检测该涂层的物理表征;检测该涂层浸提液的离子浓度;用不同稀释比例的浸提液培养MC3T3-E1细胞,观察细胞形态,MTT法检测细胞相对增殖率,并检测细胞凋亡水平;测定溶血率。结果 该涂层浸泡72 h后,浸提液中钙、硅、锌离子浓度均明显升高;用不同稀释比例的浸提液培养的MC3T3-E1细胞都表现出正常的细胞形态,其中3.125%、6.25%组细胞量较多,相对增殖率高于阳性对照组,且细胞凋亡率低于阳性对照组(完全培养基);随浸提液浓度增加,可对细胞增殖产生轻度抑制作用,凋亡率逐渐升高;溶血试验结果显示,该涂层浸提液的溶血率为0.5%。结论 锌修饰硅酸钙生物陶瓷涂层没有细胞毒性、生物相容性好,具有作为骨植入体及其他生物材料表面改性的生物学基础。