中药厚朴及其类似品的有效成分分析

本文对中药厚朴及其类似品中的有效成分——厚朴酚、和厚朴酚及木兰箭毒碱分别进行了高效液相色谱法和双波长扫描法测定;对其挥发油中化学成分进行了气-质联用分离与鉴定。     

高效液相色谱法测定午时茶胶囊中厚朴酚与和厚朴酚含量

目的 :建立中成药午时茶胶囊中厚朴酚与和厚朴酚含量测定的高效液相色谱法。方法 :色谱条件为 AlltimeC1 8(Alltech 5μm,4.6mm× 2 5 mm)柱 ,流动相 :甲醇 -水 (72∶ 2 8) ;流速 :1.0 ml/ min;检测波长 :2 94nm。结果 :测得厚朴酚与和厚朴酚加样回收率为 99.82 % (RSD=0 .89% ,n=6)。结论 :该法可为午时茶胶囊的质量控制和制备工艺提供依据     

降浊健美颗粒质量标准研究

目的：建立降浊健美颗粒的质量标准。方法：对山楂、火麻仁、陈皮进行了薄层鉴别；用HPLC法测定了降浊健美颗粒中厚朴酚、和厚朴酚的含量。结果：厚朴酚平均回收率为98．2％，RSD为2．35％；和厚朴酚平均回收率为98．9％，RSD为2．7％。结论：方法简便、准确、重复性好，可用于控制该制剂的质量。     

高效液相色谱法测定舒金克喘胶囊中和厚朴酚与厚朴酚的含量

目的：测定舒金克喘胶囊中和厚朴酚与厚朴酚的含量。方法：色谱条件：色谱条件与系统适用性实验：Hypersil ODS2 C18分析柱（150mm 4．6mm，5μm）；流动相：甲醇：水=（78：22），检测波长294nm流速1．0ml／min，室温操作。理论塔板数按厚朴酚计算应不低于3000。结果：和厚朴酚与厚朴酚平均回收率分别为99．62%（RSD=1．34％），98．56％（RSD=1．49%）。结论：方法快速准确，样品处理简便易行。     

Discovery of soluble epoxide hydrolase inhibitors from natural products

With the goal of developing soluble epoxide hydrolase (sEH) inhibitors with novel chemical structures, the sEH inhibitory activities of 30 natural compounds were evaluated using both a fluorescent substrate, 3-phenyl-cyano(6-methoxy-2-naphthalenyl)methyl ester- 2-oxiraneacetic acid, and a physiological substrate, 14,15-epoxyeicosatrienoic acid. To evaluate the selectivity of sEH inhibition, the inhibition of microsomal epoxide hydrolase (mEH), which plays a critical role in detoxification of toxic epoxides, was determined using human liver microsomes. Honokiol and β-amyrin acetate, isolated from Magnolia officinalis and Acer mandshuricum, respectively, displayed strong inhibition of sEH activity, with respective IC₅₀ values of 0.57 μM and 3.4 μM determined using the fluorescent substrate, and 1.7 μM and 6.1 μM determined using 14,15-epoxyeicosatrienoic acid. mEH activity was decreased to 49% or 61% of control activity by 25 μM honokiol or β-amyrin acetate, respectively. These results suggest that β-amyrin acetate and honokiol exhibit sEH inhibitory activity, although their sEH selectivity should be improved.     

GC测定香砂平胃丸中苍术素、厚朴酚、和厚朴酚的含量

摘 要 目的： 建立香砂平胃丸中苍术素、厚朴酚、和厚朴酚的气相色谱含量测定方法。方法: ：采用HP-5毛细管柱（30 m×0.32 mm×0.25μm）,以氮气为载气,流速为2.0 ml·min^-1,进样口温度为300℃,检测器(FID)温度为300℃,进样量为1 μl,分流比为20∶1;采用程序升温模式,起始温度为100℃,保持7 min,以20℃·min^-1的速率升温至250℃,保持10 min。结果: 苍术素、厚朴酚、和厚朴酚分别在4.264～85.280 μg·ml^-1（r=0.999 7）、9.856～197.120 μg·ml^-1（r=0.999 5）、10.040～200.800 μg·ml^-1（r=0.999 6）范围内线性关系良好,平均回收率分别为98.2%、98.6%、99.3%,RSD分别为1.5%、1.5%、1.9%（n=6）。结论:该方法简便、准确、可靠,可用于香砂平胃丸的质量控制。     

Acute and sub-chronic toxicity studies of honokiol microemulsion

The purpose of this study was to investigate the acute and sub-chronic toxicity of honokiol microemulsion. In the acute toxicity tests, the mice were intravenously injected graded doses of honokiol microemulsion and were observed for toxic symptoms and mortality daily for 14 days. In the sub-chronic toxicity study, rats were injected honokiol microemulsion at doses of 100, 500, 2500 μg/kg body weight (BW) for 30 days. After 30 days treatment and 14 days recovery, the rats were sacrificed for hematological, biochemical and histological examination. In the acute toxicity tests, the estimated median lethal dosage (LD₅₀) was 50.5 mg/kg body weight in mice. In the sub-chronic toxicity tests, the non-toxic reaction dose was 500 μg/kg body weight. In each treatment group, degeneration or/and necrosis in vascular endothelial cells and structure change of vessel wall can be observed in the injection site (cauda vein) of a few animals while there were no changes in the vessels of other organs. The overall findings of this study indicate that the honokiol microemulsion is non-toxic up to 500 μg/kg body weight, and it has irritation to the vascular of the injection site which should be paid attention to in clinical medication.     

高效液相色谱法测定加味藿香正气丸中厚朴酚与和厚朴酚的含量

目的 建立加味藿香正气丸中厚朴酚与和厚朴酚含量测定的高效液相色谱（HPLC）分析方法.方法 采用 Agilent ZORBAX SB-C18（4.6 mm×150.0 mm,5 μm）色谱柱,流动相为甲醇-水（70∶30）,检测波长为294 nm,流速为1.0 mL/min,进样量10 μL,柱温25 ℃.结果 厚朴酚与和厚朴酚的线性范围分别为0.113 0～1.130 0 μg[相关系数（r）=0.999 99,n=6]和0.061 5～0.615 0 μg（r=0.999 98,n=6）;平均回收率分别为98.4%[相对标准偏差（RSD）=0.98%,n=9]和98.8%（RSD=0.54%,n=9）.结论 HPLC法测定加味藿香正气丸中厚朴酚与和厚朴酚的含量,操作简便、准确、重现性好,可作为该药品质量控制的方法.     

HPLC法测定胃健宁胶囊中和厚朴酚及厚朴酚的含量

目的建立胃健宁胶囊中和厚朴酚及厚朴酚的检测方法。方法色谱条件为C18色谱柱;流动相:乙腈-水-冰醋酸(60∶40∶1)(V∶V∶V);流速:1.000 mL·min-1;检测波长:294 nm。结果胃健宁胶囊中和厚朴酚在0.02⁰.32μg(r=0.999 6)范围内线性关系良好,平均回收率为96.58%,RSD为1.78%(n=9);厚朴酚在0.019 60.32μg(r=0.999 6)范围内线性关系良好,平均回收率为96.58%,RSD为1.78%(n=9);厚朴酚在0.019 6⁰.313 6μg(r=0.999 8)范围内线性关系良好,厚朴酚的平均回收率为97.29%,RSD为1.39%(n=9)。结论该方法简便,准确度高,可有效控制胃健宁胶囊的质量。     

液相微萃取／后萃取-高效液相色谱法测定厚朴及其制剂中厚朴酚与和厚朴酚的含量

本文采用液相微萃取／后萃取（LPME／BE）与高效液相色谱联用法萃取并测定了中药厚朴及其制剂-藿香正气口服液和香砂养胃丸中厚朴酚与和厚朴酚的含量。优化了萃取效率影响因素，萃取溶剂、供相与接受相、转速及萃取时间。厚朴酚与和厚朴酚的线性范围分别为1．56—156μg/mL和1．10—110μg／mL；检测限（S／N=3）分别为0．10ug/mL和0.07μg/mL，回收率范围为98.3％-105．1％，RSD〈2．5％。