糖尿病足中医研究进展

WHO对糖尿病足（DF）的定义为：糖尿病（DM）合并神经病变及不同程度末梢血管病变而导致下肢感染、溃疡形成和／或深部组织的破坏。DM患者中〉15％将在其生活的某一时间发生足溃疡或坏疽。近年来我国学者通过中医药治疗，明显减少DF的截肢率和死亡率。现将这方面的进展综述如下。     

正交试验优选氯仿萃取法提取胆红素工艺

用正交设计试验,对从猪胆汁提取胆红素的工艺条件进行优选。结果表明,以胆汁水解时ρH为11.00,温度为101℃(微沸),9min,萃取时ρH为5.50为佳。     

子宫肌瘤组织中胰岛素样生长因子-Ⅰ和Ⅱ mRNA表达及意义

近年来研究表明:一些生长因子可能是通过介导女性激素,以改变子宫肌细胞中细胞癌基因和肿瘤抑制基因的活性而促发子宫肌瘤[1].本研究应用半定量RT-PCR方法检测子宫肌瘤组织与正常子宫肌组织中胰岛素样生长因子-Ⅰ(IGF-Ⅰ)mRNA、胰岛素样生长因子-Ⅱ(IGF-Ⅱ)mRNA表达水平,并分析其表达与月经周期的关系,旨在探讨IGF-Ⅰ,IGF-Ⅱ与子宫肌瘤发生、发展的关系,以期进一步阐述子宫肌瘤的生长机制.     

PET/CT在评价及观察大鼠肺癌放疗效果中的应用

Objective The aim of this study was to understand the role of PET/CT in monitoring the therapeutic effect of radiotherapy (RT) on lung cancer with Wistar rats. Methods Thirty Wistar in-bred strain rats (6-8 weeks, weighed 180-280 g, female, ordinary) were made into Lewis pulmonary tumor model rats. 18F-fluorodeoxyglucose (FDG) PET/CT was performed when tumor reached 1.5-2.0 cm in greatest diameter (4-6 weeks) as a baseline. In order to get the optimal time point of PET/CT for moni-toring RT effect in rat cancer model, PET/CT was performed at the 3rd day, 1st, 2nd, 3rd, and 4th week after giving single dose of 5 Gy to each rat. Standardized uptake values (SUV) from FDG PET were measured and rats were sacrificed at different time point for validation. Besides, the expressions of glucose transport1 (Glut1) in tumor tissue were studied using immunohistochemistry. The level of tumor cell apoptosis, degra-dation, and necrosis were observed. SPSS 11.0 software was used for data analyses. Results A negative correlation of SUV uptake and time after RT and negative correlation of Glut1 expression and time after RT were observed in rat tumors, respectively. Positive correlation of SUV uptake and Glut1 expression in rat tumors was observed (Spearman rank correlation test, rs = 0.97, P < 0.01). Before RT, the SUV in rat tumor was 1.28 ± 0.31 and was decrease to 1. 00 ± 0.23 at the 3rd day and 0.18 ± 0. 10 at the 4th week after RT (F=15. 126, P<0.05). Before RT, the Glut1 in rat tumor was 0.2558 ±0.03 and was decrease to 0. 2320 ± 0. 01 at the 3rd day and 0. 1320±0.04 at the 4th week after RT. The amounts of tumor cell apopto-sis, degradation, and necrosis increased with time after RT. Conclusion Though FDG PET could monitor the therapeutic effect at the 3rd day after giving single dose of RT to rat lung tumor model, the optimal time was the the 4th week after treatment.     

伏九贴敷治疗肺系病证时机选择理论探要

伏九贴敷疗法是一种中医外治法,本文从运气学说及"春夏养阳、秋冬养阴"学说等角度诠释天人相应理论,并提出冬病夏治、夏病冬防的伏九贴敷疗法,是"天人相应"理论与"春夏养阳、秋冬养阴"学说的有机结合和具体体现.本文通过伏九贴敷疗法时机选择的中医理论探讨,将时间医学融入中医药外治法,旨在提高临床疗效,为中医药特色疗法临床实践开辟更加广阔的前景.     

白血病抑制因子反义寡核苷酸对髓母细胞体外生长的抑制作用

为进一步认识白血病抑制因子（LIF）对人髓母细胞瘤生长的生物学作用，我们使用LIF反义寡核苷酸，在髓母细胞内特异性阻断LIF基因表达并观察它对靶细胞的生物学效应。结果发现，被处理细胞的LIF表达降低至RT／PCR检出的阈值之下，抗LIF的免疫组织化学染色亦呈阴性，同时，这些细胞的生长速度明显减缓。相反，用与反义寡核苷酸互补的正义序列处理的细胞无论在基因表达／产生，还是在生长速度方面，均和正常培养细胞的细胞相似，本研究从而提示了一条以LIF为对象的髓母细胞瘤以及其它LIF生长依赖性肿瘤的基因和免疫治疗途径。     

PCA 在美容外科手术的应用

患者自控镇痛术(patient-controlled analgesia,PCA)治疗,使患者可以根据自己的镇痛需要,自我控制给药,将对镇痛用药的个体差异性降到最小程度,使阿片类药用药量大大减少,同时其剂量相关性不良反应的发生率降低.     

STD患者的行为特征及健康教育对策

长期以来 ,性传播疾病 (ＳＴＤ)一直是全球性的公共卫生问题和社会卫生问题 ,其中危害最严重的是没有有效疫苗和治疗方法的艾滋病 (ＡＩＤＳ) ,1 999年底全球报告的艾滋病患者已达 2 2 0万 ,估计艾滋病病毒ＨＩＶ感染人数为 336 0万人。 2 0 0 1年全国ＨＴＶ感染人数近 1 0 0万