缺血修饰白蛋白在射频消融术前后的变化及其临床意义

目的观察心律失常患者射频消融术所诱导的短暂心肌缺血中血清缺血修饰白蛋白、血浆心肌肌钙蛋白T及肌酸激酶同工酶的浓度变化及其临床意义。方法112例行射频消融术的心律失常患者（射频消融组）,分别测定术前和术后3、6h血清缺血修饰白蛋白、血浆心肌肌钙蛋白T及肌酸激酶同工酶的浓度,并与91例行冠状动脉造影证实冠状动脉正常患者（对照组）术前和术后3、6h的上述指标的检测结果进行对比分析。结果射频消融组患者的血清缺血修饰白蛋白术后3h[（80．62±7．53）U／ml]、6h[（76．37±9．21）U／ml]较术前[（45．42±7．00）U／ml]升高（P均〈0．05）,而对照组患者的缺血修饰白蛋白术前、术后比较差异无统计学意义（P〉0．05）。两组患者血浆心肌肌钙蛋白T及肌酸激酶同工酶术前及术后3、6h差异均无统计学意义（P均〉0．05）。结论缺血修饰白蛋白可在心肌发生缺血缺氧损伤的射频消融术后迅速升高,并早于心肌肌钙蛋白T。缺血修饰白蛋白是早期诊断心肌缺血的敏感生化指标。     

Molecular immune recognition of botulinum neurotoxin B. The light chain regions that bind human blocking antibodies from toxin-treated cervical dystonia patients. Antigenic structure of the entire BoNT/B molecule

We recently mapped the regions on the heavy (H) chain of botulinum neurotoxin, type B (BoNT/B) recognized by blocking antibodies (Abs) from cervical dystonia (CD) patients who develop immunoresistance during toxin treatment. Since blocking could also be effected by Abs directed against regions on the light (L) chain, we have mapped here the L chain, using the same 30 CD antisera. We synthesized, purified and characterized 32 19-residue L chain peptides that overlapped successively by 5 residues (peptide L32 overlapped with peptide N1 of the H chain by 12 residues). In a given patient, Abs against the L chain seemed less intense than those against H chain. Most sera recognized a limited set of L chain peptides. The levels of Abs against a given region varied with the patient, consistent with immune responses to each epitope being under separate MHC control. The peptides most frequently recognized were: L13, by 30 of 30 antisera (100%); L22, by 23 of 30 (76.67%); L19, by 15 of 30 (50.00%); L26, by 11 of 30 (36.70%); and L14, by 12 of 30 (40.00%). The activity of L14 probably derives from its overlap with L13. The levels of Ab binding decreased in the following order: L13 (residues 169-187), L22 (295-313), L19 (253-271), and L26 (351-369). Peptides L12 (155-173), L18 (239-257), L15 (197-215), L1 (1-19) and L23 (309-327) exhibited very low Ab binding. The remaining peptides had little or no Ab-binding activity. The antigenic regions are analyzed in terms of their three-dimensional locations and the enzyme active site. With the previous localization of the antigenic regions on the BoNT/B H chain, the human Ab recognition of the entire BoNT/B molecule is presented and compared to the recognition of BoNT/A by human blocking Abs.     

Oral treatment with Hev b 13 prevents experimental arthritis in mice

Hev b 13 is an allergenic esterase obtained from the rubber tree Hevea brasiliensis, which has been shown recently to induce human monocytes to release interleukin (IL)-10 in vitro, and to exert a potent anti-inflammatory effect in vivo. Moreover, Hev b 13 has been shown to reduce clinical signs of inflammation and also histological damage to the distal colon of mice with 2,4,6-trinitrobenze sulphonic acid (TNBS)-induced colitis after its oral administration. The aim of this study was to investigate the effect of Hev b 13 on human mononuclear cells, as well as its therapeutic use in the methylated bovine serum albumin (mBSA) model of antigen-induced arthritis. Five days before the intra-articular challenge, and daily thereafter for 8 days, Hev b 13 was administered by oral gavage. In mice treated with a dose of 0·5 mg/kg of Hev b 13, the severity of oedema, leucocyte infiltration, pannus formation and cartilage erosion were reduced significantly. These findings underscore the anti-inflammatory activity suggested previously for Hev b 13, an activity speculated to be related to its interaction with monocytes/macrophages and the consequent stimulation of IL-10 release and reduction of tumour necrosis factor (TNF) release. The study also opens a wide range of possible applications in the field of immune-mediated inflammatory diseases.     

缺血修饰自蛋自在诊断急性心肌缺血中的作用

目的探讨缺血修饰白蛋白（IMA）在诊断急性心肌缺血中的应用。方法采用血清蛋白钴结合试验检测急性心肌缺血患者的IMA水平。检测120例急性心肌缺血患者2h内及30例正常健康对照组的血清IMA水平,结合患者临床资料进行分析。结果各组冠心病患者SA、UA和AMI与对照组相比较,血清IMA水平显著升高,差异有统计学意义（P〈0．05）。对ST段变化进行比较,两组IMA的结果有差异,但差异无统计学意义（P〉0．05）。SA与UA两组患者ST段的压低或抬高对血清IMA的值没有影响。结论IMA对于急性心肌缺血具有早期诊断意义,并可评估心绞痛患者的缺血严重程度。     

人血清白蛋白?鄄粒细胞集落刺激因子融合蛋白对化疗大鼠的治疗作用

目的 探索重组人血清白蛋白?鄄粒细胞集落刺激因子融合蛋白（GW003)对环磷酰胺（CTX)化疗大鼠的治疗作用。方法 80只SD大鼠经CTX化疗后分别一次皮下注射生理盐水和不同剂量GW003（250、500和1500 μg/kg）,每日一次检测外周血细胞计数,药后第10天处死大鼠取胸骨行病理组织学观察。结果 SD大鼠腹腔注射CTX后外周血白细胞、中性粒细胞、淋巴细胞和血小板数均显著下降。GW003治疗可明显升高化疗大鼠外周血白细胞和中性粒细胞数的最低值并缩短其减少的持续时间。GW003对CTX化疗大鼠外周血淋巴细胞、红细胞和血小板数无明显影响。结论 GW003对CTX化疗大鼠外周血白细胞减少有明显的治疗作用。     

Characterization of the baicalein-bovine serum albumin complex without or with Cu2+ or Fe3+ by spectroscopic approaches

The binding of baicalein to bovine serum albumin (BSA) in the absence and presence of Cu²⁺ or Fe³⁺ in aqueous solution has been studied by fluorescence, synchronous fluorescence, ultraviolet-visible (UV-vis) spectra, circular dichroism (CD) and the three-dimensional (3D) fluorescence at pH 7.40. The decrease of the binding constant in the presence of Cu²⁺ or Fe³⁺ may result from the competition of the metal ions and baicalein binding to BSA. The effect of baicalein on the conformation of BSA was analyzed using UV, CD, fluorescence and three-dimensional (3D) fluorescence. These results indicate that the binding of baicalein to BSA causes apparent change in the secondary structure of BSA, but does not affect the polarity around the chromophore molecule.     

基于血清蛋白的沙蟾毒精与盐酸维拉帕米相互作用研究

目的：研究沙蟾毒精和盐酸维拉帕米与血清蛋白的结合及两者间的相互影响。方法：采用平衡透析法和高效液相色谱法测定两种药物与小牛血清蛋白的结合率。荧光光谱法研究沙蟾毒精与牛血清白蛋白和人血清白蛋白（human serumalbumin,HSA）的相互作用,并采用分子对接模拟药物相互作用模式。结果：0.1μg/mL沙蟾毒精与小牛血清蛋白结合率为（61.1±0.2）%,加入不同浓度盐酸维拉帕米后,沙蟾毒精的血清蛋白结合率显著下降,从（61.1±0.2）%下降到（36.9±3.4）%。测得盐酸维拉帕米（2μg/mL）的血清蛋白结合率为（87.5±0.5）%;当加入不同浓度沙蟾毒精后,盐酸维拉帕米血清蛋白结合率无显著变化。荧光光谱检测表明盐酸维拉帕米能够抑制沙蟾毒精与血清白蛋白的结合。分子对接结果表明,沙蟾毒精、盐酸维拉帕米竞争性结合HSA的位点Ⅰ,盐酸维拉帕米与位点Ⅰ的分子结合能力大于沙蟾毒精,置换沙蟾毒精与HSA在位点Ⅰ的结合。结论：盐酸维拉帕米能够抑制沙蟾毒精与血清蛋白及白蛋白的结合。     

2型糖尿病肾病患者外周血单个核细胞组织因子活性检测的意义

目的探讨2型糖尿病肾病患者外周血单个核细胞组织因子促凝活性(TF-PCA)的改变及其临床意义。方法 63名2型糖尿病患者按照尿白蛋白/肌酐比共分为三组:比值<30mg/g为正常蛋白尿组(n=22),30mg/g≤比值<300mg/g为微量白蛋白尿组(n=26),比值≥300mg/g为大量白蛋白尿组(n=15);同时选择21名健康体检者作为对照组。采用一期凝固法测定外周血单个核细胞TF-PCA。常规检测空腹血糖、糖化血红蛋白、超敏CRP(Hs-CRP)、尿酸(UA)、胱抑素C(CYSC),并进行相关分析。结果糖尿病组外周血TF-PCA随尿蛋白水平升高而升高,同时单个核细胞TF-PCA与空腹血糖、Hs-CRP、UA等呈正相关,相关系数分别为0.419、0.293、0.232(P<0.05)。结论 2型糖尿病患者外周血单个核细胞TF-PCA明显升高,且与多种因素相关,TF-PCA升高可能与DN的发病机制、病程进展相关。     

大黄蜇虫丸影响免疫性肝纤维化大鼠TIMP-1表达的动态观察

目的观察大黄蜇虫丸对免疫性肝纤维化大鼠TIMP-1表达影响的动态变化,探讨大黄蜇虫丸抗肝纤维化的作用机制。方法将120只健康雄性SD大鼠随机分为6组。即正常组,模型组,大黄蜇虫丸低剂量组,大黄蜇虫丸中剂量组,大黄蜇虫丸高剂量组,秋水仙碱组,各20只,除正常组外其它各组均注射人血清白蛋白制作免疫性肝纤维化大鼠模型,模型制作完成后,各组采用相应的药物连续干预4周,分别于药物治疗后1、2、3、4周末随机处死5只大鼠,用酶联免疫吸附法检测血清中基质金属蛋白酶抑制因子-1(TIMP-1)的含量变化。结果①模型组随着周次的延长血清TIMP-1同步增高,差异有统计学意义(P<0.01);②大黄蜇虫丸低剂量组、大黄蜇虫丸中剂量组、大黄蜇虫丸高剂量组随着周次的延长血清TIMP-1同步降低,差异有统计学意义(P<0.01),其中大黄蜇虫丸中剂量组优于其他两组(P<0.01);③大黄蜇虫丸有明显的抑制肝纤维化模型大鼠肝组织TIMP-1表达的作用,且抑制作用要优于秋水仙碱组(P<0.01)。结论大黄蜇虫丸可有效防治大鼠免疫性肝纤维化,其作用机制可能是通过抑制肝纤维化组织TIMP-1的表达而实现。     

不同ACEI类药物对糖尿病大鼠TGFβ1水平的影响

目的:观察洛汀新(盐酸贝那普利,Benazepril Hydrochloride Tablets)、依那普利(Enalapril)和卡托普利(Captopril)对糖尿病大鼠转化生长因子β1(transforming growth factor beta 1,TGFβ1)水平的影响.方珐:建立糖尿病大鼠模型,分为正常对...