电针改善硬膜外吗啡用于术后镇痛所引起的免疫抑制

为观察硬膜外吗啡和电针对术后患者免疫功能的影响，检测自然杀伤细胞（ＮＫｃｅｌｌ）活性和ＰＨＡ诱导白细胞介素２（ＩＬ－２）水平在单纯胆囊切除术患者术前和术后第１、３、７天的动态变化情况。结果吗啡组ＮＫ活性在术后第１、３、７天抑制，手术组仅在术后第１、３天出现抑制，而抑制率低于同天的吗啡组，电针可拮抗吗啡引起的ＮＫ活性抑制加深状况。在术后第１天，手术组和吗啡组ＩＬ－２水平均下降，吗啡＋电针组无明显变化，术后第７天吗啡＋电针组ＩＬ－２升高接近正常人水平。表明电针能改善硬膜外吗啡引起的免疫抑制，促进术后机体的恢复。硬膜外吗啡结合电针是值得推荐的术后镇痛方法。     

115 BRCA1基因与乳腺癌研究进展

乳腺癌易感基因BRCA1作为抑癌基因,其编码产物在细胞周期调控、DNA损伤修复以及肿瘤细胞的凋亡中扮演着重要角色.大量研究证明,BRCA1基因结构、功能异常与乳腺癌的发生发展有十分密切的联系.本文拟就BRCA1与乳腺癌相关研究的最新进展作一综述.     

Molecular analysis and polymorphism of the DLA-DQB genes

Abstract: Partial-length cDNA clones and full-length genomic clones corresponding to a complete canine DQB class It gene were isolated. Southern analyses suggested the presence of two DQB genes - one of which appeared to be a pseudogene lacking exon 2 called DQB2. The other DQB gene, called DQB1, was isolated from a genomic phage clone and contained six exons. The DQB1 clone was restriction mapped, and exon 2 was sequenced from 70 dogs. Twenty alleles were found. Most of the amino acid substitutions occurred at putative positions in the peptide binding site. Inheritance of these sequences showed Mendelian segregation with one or two alleles per dog. Cluster analysis of the nucleotide and predicted amino acid sequences subdivided the canine DQB1 alleles into four major allelic groups. The number of nonsynonymous changes was higher than the number of synonymous changes in the putative antigen recognition sites suggestive of positive selection.     

复方甘油注射液长期毒性试验

复方甘油注射液系新脱水药。为了确定该药反复使用有无蓄积性中毒作用及最大无作用量，作者以大鼠和狗进行为期２８天的长期毒性试验。两种动物皆设立生理盐水对照组，低剂量组（大鼠为１０ｍｌ／ｋｇ，狗为１．７ｍｌ／ｋｇ），高剂量组（大鼠为４５ｍｌ／ｋｇ，狗为１３ｍｌ／ｋｇ）。按规定剂量每天一次静注给药，连续２８天。结果表明，两种动物均未出现明显的机能性变化，以及特异性靶器官、靶细胞的损害。故认为大鼠和狗对受试物的最大无作用量分别大于４５ｍｌ／ｋｇ和１３ｍｌ／ｋｇ。     

Expression of Human Recombination Activating Genes (RAG-1 and RAG-2) in Lymphoma

Two recently discovered genes, the recombination activating genes 1 and 2 (RAG-1 and RAG-2), are necessary to perform variable (V), diversity (D), and joining (J) recombination. They synergistically activate VDJ recombination to generate immunocompetent lymphocytes. Disruption of either gene results in a maturation arrest at a very early B and T cell progenitor stage. Expression and downregulation of RAG's are closely associated with interleukin 7, sIgM and TCR-CD3 complex, respectively. Assessment of RAG mRNA expression is a valuable marker in identifying the genotypic maturation status of leukemias and lymphomas. Persistent RAG expression in otherwise mature lymphoid proliferations may explain puzzling biological and clinical observations such as multiple rearrangements in lymphomas with a mature phenotype. Lack of RAG expression in Hodgkin's disease with abundant Reed-Stern-berg cells is consistent with a mature phenotype of the latter. Availability of a anti-RAG-1 monoclonal antibody in the near future will facilitate RAG analysis of lymphomas.     

苯DNA加合物研究进展(综述)

苯ＤＮＡ加合物研究进展（综述）李桂兰，常平综述徐伯洪审校（中国预防医学科学院劳动卫生与职业病研究所，北京１０００５０）我国职业接触苯的工人约有５０万，长期接触苯可引起白血病。苯与其它实质性肿瘤的问题也有报道［１，２］。世界癌症研究机构（ＩＡＲＣ）１９...     

聚对苯乙炔（PPV）及其衍生物的研究进展

评价了ＰＰＶ系列衍生物的电学性质、非线性光学性质及其应用。     

Cytokine Production by Lymphocytes in Pregnancy

PROBLEM: In the presence of progesterone lymphocytes of pregnant women release a 34- kDa protein named the progesterone-induced blocking factor (PIBF). PIBF mediates the immunomodulatory and anti-abortive effects of progesterone and its presence is related to the outcome of pregnancy. PIBF induces production of Th2 type cytokines by activated lymphocytes. The in vivo relationship between PIBF- and cytokine production of pregnancy lymphocytes and the outcome of pregnancy was investigated. METHOD OF STUDY: Interleukin (IL)-12 and IL-10 production and PIBF expression in peripheral lymphocytes of 111 healthy pregnant women and 120 women at risk for premature pregnancy termination were detected by immunocytochemistry. RESULTS: We found increased IL-12 and low PIBF and IL-10 expression on lymphocytes of “risk” patients, and a high rate of IL-10 and PIBF positivity on lymphocytes from healthy pregnant women. The cytokine production pattern of the lymphocytes was related to the presence or absence of previous abortions as well as to the outcome of pregnancy. CONCLUSION: These data suggest the involvement of an altered cytokine production pattern in the immunologic effects of progesterone.     

中国人丙型肝炎病毒囊膜蛋白2HVR1 cDNA的序列分析

目的：了解中国ＨＣＶ流行株高变区１（ＨＶＲ１）的特点。方法：对来自山东（ＳＤ）、上海（ＳＨ）两地患者血清ＲＮＡ进行逆转录－巢式ＰＣＲ，扩增ＨＣＶ囊膜蛋白２基因片段，用ＰＣＲ直接测序法对该片段进行序列测定。结果：（１）扩增片段（命名为Ｐ３８８）对应于日本ＨＣＶ－Ｊ株序列核苷酸１４３９￣１８２６位（氨基酸位３７１￣４９８）；（２）中国人ＨＣＶ ＨＶＲ１位于氨基酸位３８４￣４１０，与发表的ＨＣＶⅡ型ＨＶ