神经生长因子对促进种植体骨结合的研究进展

［摘要］ 神经生长因子（nerve growth factor,NGF）广泛分布于中枢神经系统及周围神经系统中,具有促进血管生长、创伤愈合和神经系统发育以及骨形成的作用。影响种植体骨结合稳定性的因素很多,种植体表面的性质是影响种植体骨结合的主要因素。NGF在种植体骨结合过程中具有增强骨细胞活性,促进成骨细胞分化、矿化,以及促进周围神经、血管形成的作用,它能够有效地促进种植体周围骨愈合,从而缩短口腔种植体骨愈合时间。本文就神经生长因子促进种植体骨结合的研究进展进行综述。     

CXCL10 and its related key genes as potential biomarkers for psoriasis: Evidence from bioinformatics and real-time quantitative polymerase chain reaction

Although several studies have attempted to investigate the etiology of and mechanism underlying psoriasis, the precise molecular mechanism remains unclear. Our study aimed to explore the molecular mechanism underlying psoriasis based on bioinformatics.GSE30999, GSE34248, GSE41662, and GSE50790 datasets were obtained from the Gene Expression Omnibus database. The Gene Expression Omnibus profiles were integrated to obtain differentially expressed genes in R software. Then a series of analyses was performed, such as Gene Ontology annotation, Kyoto Encyclopedia of Genes and Genomes pathway analysis, protein-protein interaction network analysis, among others. The key genes were obtained by CytoHubba, and validated by real-time quantitative polymerase chain reaction.A total of 359 differentially expressed genes were identified between 270 paired lesional and non-lesional skin groups. The common enriched pathways were nucleotide-binding and oligomerization domain-like receptor signaling pathway, and cytokine-cytokine receptor interaction. Seven key genes were identified, including CXCL1, ISG15, CXCL10, STAT1, OASL, IFIT1, and IFIT3. These key genes were validated as upregulated in the 4 datasets and M5-induced HaCaT cells.Our study identified 7 key genes, namely CXCL1, ISG15, CXCL10, STAT1, OASL, IFIT1, and IFIT3, and 2 mostly enriched pathways (nucleotide-binding and oligomerization domain-like receptor signaling pathway, and cytokine-cytokine receptor interaction) involved in psoriatic pathogenesis. More importantly, CXCL1, ISG15, STAT1, OASL, IFIT1, IFIT3, and especially CXCL10 may be potential biomarkers. Therefore, our findings may bring a new perspective to the molecular mechanism underlying psoriasis and suggest potential biomarkers.     

基于生物信息学筛选脓毒症中性粒细胞活化的关键基因

目的基于生物信息学筛选脓毒症中性粒细胞活化的关键基因。 方法从GEO数据库中下载4个成人脓毒症芯片数据集(GSE69528、GSE28750、GSE57065、GSE95233),进行差异表达基因(DEG)筛选。基因本体论(GO)分析DEG,STRING数据库构建蛋白互作网络,Cytoscape软件提取关键基因,CIBERSORT算法估算数据集中的免疫细胞表达水平,ROC曲线评价关键基因对脓毒症患者的诊断价值。取儿童脓毒症相关芯片数据集(GSE66099)进行验证。 结果4个成人脓毒症数据集筛选出299个重叠DEG,其中112个基因上调,187个基因下调。GO主要富集于中性粒细胞活化、中性粒细胞脱颗粒、肽酶调节活性、糖胺聚糖结合、特殊颗粒、囊泡腔等。筛选出脓毒症中性粒细胞活化的关键基因10个,在脓毒症中表达水平均上调,ROC曲线下面积均大于0.7,具有较好的诊断价值。儿童脓毒症数据集验证结果与成人脓毒症数据集结果一致。 结论基于生物信息学方法筛选出脓毒症中性粒细胞活化相关的10个关键基因,为脓毒症的早期诊断、预后判断及治疗提供了潜在新靶点。     

膝关节OA关键基因及软骨组织免疫细胞浸润的生物信息学分析

目的基于生物信息学筛选膝关节骨关节炎(OA)的关键基因、信号通路,并分析软骨组织免疫细胞浸润情况。 方法从GEO数据库中下载来自正常人和OA患者的基因数据集,进行差异表达基因筛选,基因本体论、京都基因和基因组百科全书分析,构建蛋白互作网络,并对软骨相关数据集进行免疫细胞浸润分析。 结果筛选出与OA相关的12个关键基因及86条关键信号通路。免疫浸润结果显示,OA软骨组织与正常软骨比较,其活化CD8T记忆细胞、CD56⁺NK细胞、髓系来源抑制性细胞、1型辅助型T细胞4种免疫细胞含量差异有统计学意义(P＜0.05)。 结论筛选出12个关键基因和4种免疫细胞,其可能参与了OA的进展。     

中国车前草的形态鉴定

本文对我国产18种车前草的植物形态进行了鉴定研究,其中有6种在国内首次报道。文中附18种车前草植物图及形态鉴定检索表。     

浅谈针刺补泻操作的关键

通过对各种针刺补泻手法的分析,结合实验验证,在得气的基础上,针刺补泻操作的关键可能是其作用于躯体的趋势,即向(体)内趋势为主的操作手法为补,向(体)外趋势为主的操作手法为泻。     

品牌管理对医院重点学科建设的作用

在政府主导和市场选择等因素的共同作用下，医院开展品牌管理促进学科建设成为必然。以品牌战略理论为指导的医院品牌管理对重点学科建设具有促进作用。应从医院战略管理、学科建设组织体系与运行机制、医院品牌推广等方面进行重点学科建设。     

Effects of a Gonadotropin-releasing Hormone Agonist on Rat Ovarian Adenocarcinoma Cell Lines in vitro and in vivo

To evaluate the biologic effects of the gonadotropin-releasing hormone (GnRH) agonist buserelin on rat ovarian adenocarcinoma cells in vivo and in vitro , female Wistar rats with primary ovarian adenocarcinoma induced by 7, 12-dimethylbenz( a )anthracene (DMBA) and the DMBA-OC-1 cell line established from a DMBA-induced rat tumor were used in this study. In vivo , daily administration of buserelin significantly suppressed the release of follicle-stimulating hormone (FSH), luteinizing hormone (LH), and progesterone as compared with controls. Buserelin did not inhibit the growth of DMBA-induced tumors. However, histopathologically, there was increased central necrosis and a decrease in the number of neoplastic cells, with proliferation of connective tissue, in the group treated with buserelin. In vitro , FSH-induced proliferation of DMBA-OC-1 cells was suppressed by buserelin. Thus, this basic experimental study supports the potential use of a GnRH agonist to suppress the growth of ovarian cancer.     

Inhibitory Effects of Ginsenoside Rh2 on Tumor Growth in Nude Mice Bearing Human Ovarian Cancer Cells

Ginsenoside Rh₂ (Rh₂), isolated from an ethanol extract of the processed root of Panax ginseng CA Meyer, inhibits the growth of B16 melanoma cells. This study was designed to evaluate the ability of Rh₂ to inhibit growth of human ovarian cancer cells (HRA) in vitro and in nude mouse. Rh₂ inhibited proliferations of various established human ovarian cancer cell lines in a dose-dependent manner between 10 and 60 μM in vitro and induced apoptosis at around the IC₅₀ dose. When HRA cells were inoculated s.c. into the right flank of nude mice, all mice formed a palpable tumor within 14 days. Although i.p. administration of Rh₂ alone hardly inhibited the tumor growth, when Rh₂ was combined with cis-diamminedichloroplatinum(II) (CDDP) the tumor growth was significantly inhibited, compared to treatment with CDDP alone. When mice were treated p.o. with Rh₂ daily (but not weekly), the tumor growth was significantly (P<0.01) inhibited, compared to CDDP treatment alone. When Rh₂ was combined with CDDP, the degree of tumor growth retardation was not potentiated. The survival time was significantly (P<, we examined whether p.o. administration of Rh₂ has a dose-dependent inhibitory effect on the tumor growth. I.p. and weekly administration of CDDP had more potent antitumor activity in the order of 1 mg/kg, 2 mg/kg and 4 mg/kg, whereas p.o. and daily administration of Rh₂ (0.4 to 1.6 mg/kg) not only had antitumor activity comparable to that of 4 mg/kg CDDP, but also resulted in a significant increase of the survival. Doses of Rh₂ used in this study did not result in any adverse side-effects as confirmed by monitoring hematocrit values and body weight, unlike 4 mg/kg CDDP, which had severe side-effects. It is noteworthy that p.o. but not i.p. treatment with Rh₂ resulted in induction of apoptotic cells in the tumor in addition to augmentation of the natural killer activity in spleen cells from tumor-bearing nude mice. Thus, particularly in view of the toxicity of CDDP, Rh₂ alone would seem to warrant further evaluation for treatment of recurrent or refractory ovarian tumor.     

生物制品生产设备检查要点探析

近年来，在国内外生物制品生产现场检查中发现生产设备方面的问题较为突出。对生物制品生产设备的检查是基于药品生产质量管理规范（GMP）标准规定开展的，因此对无菌生物制品原液生产设备、制剂生产设备和一次性使用系统的检查要点进行总结和分析，并列举了国内外生物制品检查典型问题，探讨在各类检查和自查中如何提高对生物制品生产设备的检查实效，同时也为生物制品行业进一步加强生产设备的管理提供参考。