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M. D&#x;Alessandro L. Poli Q. Lai A. Gaeta C. Nazzari M. Garofalo F. Nudo F. Della Pietra A. Bachetoni V. Sargentini A. Angeloni P.B. Berloco R. Pretagostini 《Transplantation proceedings》2019,51(1):157-159
Background
BK virus (BKV)–associated nephropathy is definitely involved in allograft failure after kidney transplant. Thus, the need for an early control of viral reactivation in immunocompromised patients is well established. Determination of urinary release of decoy cells (DC) and BK viral load in plasma and urine by polymerase chain reaction (PCR) usually precedes renal biopsy. The aim of the study is to assess viral reactivation by BKV-DNA PCR and DC detection in urinary sediment using automated intelligent microscopy.Methods
Seventy-eight kidney transplant patients were analyzed for the presence of plasma BKV-DNA by quantitative TaqMan real-time PCR. Additionally, automated intelligent microscopy was used for urine sediment analysis, allowing to count cells with decoy feature, confirmed by phase contrast microscopic review.Results
Plasma BKV-DNA PCR was detected in 14 (17.9%) patients. DC were identified in 19 (24.3%) urine sediments by automated analyzers and confirmed by microscopic observation. Two patients were BKV-DNA–positive/DC-negative; conversely, 7 subjects were DC-positive/BKV-DNA–negative.Conclusions
Plasma quantification of BK viral load is currently the best noninvasive method for the detection of viral reactivation. Nevertheless, automated methods to screen for the presence of DC in urine could facilitate early BK virus replication diagnosis and patient follow-up by quantitative and visual results. 相似文献93.
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Ara Tekian Martin Borhani Sarette Tilton Eric Abasolo Yoon Soo Park 《American journal of surgery》2019,217(2):288-295
Background
This study examines the alignment of quantitative and qualitative assessment data in end-of-rotation evaluations using longitudinal cohorts of residents progressing throughout the five-year general surgery residency.Methods
Rotation evaluation data were extracted for 171 residents who trained between July 2011 and July 2016. Data included 6069 rotation evaluations forms completed by 38 faculty members and 164 peer-residents. Qualitative comments mapped to general surgery milestones were coded for positive/negative feedback and relevance.Results
Quantitative evaluation scores were significantly correlated with positive/negative feedback, r?=?0.52 and relevance, r?=??0.20, p?<?.001. Themes included feedback on leadership, teaching contribution, medical knowledge, work ethic, patient-care, and ability to work in a team-based setting. Faculty comments focused on technical and clinical abilities; comments from peers focused on professionalism and interpersonal relationships.Conclusions
We found differences in themes emphasized as residents progressed. These findings underscore improving our understanding of how faculty synthesize assessment data. 相似文献95.
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