二性霉素B脂质体的研究与应用

二性霉素Ｂ是目前治疗严重深部真菌病－隐球菌脑膜炎、系统性念珠菌病及侵袭型曲霉病等的最有效的抗真菌药物。鉴于其临床应用中毒副反应重，具有耐药倾向，将其静脉注射剂研制成脂质体，具有疗效高，毒副反应小的优点。     

糖尿病合并急性肾损伤相关心肾综合征发病机制的研究进展

围手术期糖尿病合并急性肾损伤相关心肾综合征的发病机制与肾素–血管紧张素–醛固酮系统、神经体液及环境等因素相关,但具体机制尚不明确。本文对糖尿病合并急性肾损伤相关心肾综合征发病机制的最新研究进展进行综述,以期为该疾病的临床诊疗提供理论依据。     

人体恶性实体瘤TIL增殖力、表型和杀伤力研究

在建立高活力肿瘤浸润淋巴细胞（ＴＩＬ）分离培养方法的基础上，对８３例恶性实体瘤ＴＩＬ的细胞增殖动力学、部份ＴＩＬ表型与杀伤力等作了较为系统的研究。结果提示，本实验室所建立分离培养的ＴＩＬ增殖能力较强且稳定，有６５％的增殖倍数大于１０００倍；３Ｈ－ＴｄＲ掺入高峰在培养的第４５～７５天；对其自身的原代肿瘤细胞杀伤力可以维持至５６天以上；对５６份经ｒＩＬ２诱导的ＴＩＬ（培养时间１７±５．２天）表型分析：ＣＤ３８０±２１％，ＣＤ４３７±２１％，ＣＤ８４４±１８％，ＨＬＡＤＲ６９±２４％，与ｒＩＬ２诱导前比较，ＣＤ３、ＣＤ４、ＣＤ８和ＨＬＡＤＲ均增多，尤以ＣＤ３与ＣＤ８表型增多明显。     

多巴胺D2受体基因启动子多态性与帕金森病的关联研究

目的 探讨中国汉族人群多巴胺D2受体基因启动子多态性在帕金森病（Parkinson's disease,PD)遗传易感性中的作用。方法 采用病例－对照关联分析，聚合酶链反应－限制性片段长度多态性方法分析了123例PD患者（PD组）与124名健康成人（对照组）多巴胺D2受体基因启动子多态性。结果 PD组－141△C等位基因频率为8．5％，对照组为11．7％；两组差异无显著性（P＞0．05）；中国汉族人PD组组和对照组－141△C等位基因频率明显高于意大利南部人群，差异有显著性（P＜0．05）。结论中国汉族人群多巴胺D2受体基因启动子多态性与PD的遗传易感性无关，该多态性有明显的种族差异。     

不同来源的TIL临床应用研究

以５１例晚期肿瘤患者作为研究对象，对其不同来源的肿瘤浸润淋巴细胞（ＴＩＬ）进行治疗研究，发现肿瘤组织来源的ＴＩＬ其有效率及一年生存率明显高于胸腹水及转移淋巴结的ＴＡＬ；其中肿瘤组织的ＴＩＬ，胸腹水、转移淋巴结的ＴＡＬ有效率分别为８０．９％、４０．０％、４６．６％；三者治疗肿瘤患者的一年生存率分别为８１．０％、４６．７％、６０．０％；另外发现胸腹水ＴＡＬ对实质性肿块治疗疗效较低，为４０％，其中静脉滴     

在生活的间隙中“深呼吸”

想想看:你最近是不是常常无缘无故感到疲劳,而这种疲劳休息也无济于事?你是不是会经常感冒,只要办公室有一个同事感冒,你就会马上"跟进"?还有,你是不是会不规律地头疼,从一间办公室匆匆忙忙走到另一间办公室,却忘了自己要去干什么?你失眠吗?你会无端烦恼,精神不振吗?     

Relaxin protects myocardial microvascular endothelial cells from hypoxia-reoxygenation injury北大核心

BACKGROUND: Relaxin can significantly improve cardiac and renal dysfuction caused by pathological factors, inhibit myocardial hypertrophy, have an anti-fibrosis effect, and improve ischemia-reperfusion injury. However, its protective mechanism against hypoxia-reoxygenation injury of endothelial cells remains unclear. OBJECTIVE: To investigate the protective mechanism of relaxin against hypoxia-reoxygenation injury of myocardial microvascular endothelial cells. METHODS: Mouse myocardial microvascular endothelial cell line (H5V cells) was used for the experiment. Cells were treated by three different interventions: in control group, cells were normally cultured for 33 hours; in model group, cells were treated by 6-hour hypoxia followed by 3-hour reoxygenation); and in relaxin group, 24 hours of routine culture (180 ng/mL relaxin), 6 hours of hypoxia and 3 hours of reoxygenation (180 ng/mL relaxin) were given to simulate myocardial hypoxia-reperfusion injury. Cell permeability and Caspase-3 activity were then detected. Levels of tumor necrosis factor-α, interleukin-1β and interleukin-6 in cell supernatants were detected by ELISA. Expressions of VE-cadherin, Akt, and GSK-3β at mRNA and protein levels were detected by RT-PCR and western blot, respectively. RESULTS AND CONCLUSION: Compared with the control group, the cell permeability and expression of caspase-3 increased significantly in the model group (P < 0.05). Compared with the model group, the cell permeability and expression of caspase-3 decreased in the relaxin group (P < 0.05). Moreover, the levels of tumor necrosis factor-α, interleukin-1β and interleukin-6 were elevated in the model group, while the levels were significantly decreased after relaxin treatment (all P < 0.05). There were no significantly changes in the mRNA and protein expressions of VE-cadherin, Akt1, and GSK-3β mRNA among three groups (all P > 0.05). Compared with the control group, the expression of phosphorylated VE-cadherin, Akt1 and GSK-3β were decreased in the model group (P < 0.05), and relaxin treatment reversed these changes to the control levels (P < 0.05). To conclude, relaxin treatment could enhance VE-cadherin expression, reduce hypoxiareoxygenation-induced microvascular endothelial cell damage, inhibit inflammatory cytokine release, and reduce cell apoptosis, which may be related to the activation of the Akt/GSK-3β signaling pathway. © 2023, Publishing House of Chinese Journal of Tissue Engineering Research. All rights reserved.     

深圳市民睡眠质量调查

睡眠障碍是一类常见病症。许多交通意外、生产事故均与睡眠障碍有直接关系 ,这已引起许多国家的重视。目前我国仅有少量职业人群的睡眠障碍流行病学调查报告[1 ] ,为此 ,作者首次在国内采用量表对深圳市民进行了睡眠质量调查 ,并作了测评分析。按“精神疾病流行病学调查手册”[2 ] ,提供的工具和方法 ,以当年深圳 10 3万户籍人口为调查本底 ,采用整群分层随机抽样方法 ,在对 15岁以上人口入户进行精神疾病流行学调查的同时 ,作睡眠质量调查。调查从 1996年 7月 1日开始至 12月 3 1日结束。采用匹兹堡睡眠质量指数表 (Pitts burghSleepQual…     

国人枕骨大孔及其周围某些结构的观察和测量

一、引言关于人颅枕骨大孔之形状大小及其周围的某些结构(包括髁管,舌下神经管,旁髁突及孔前缘的各种骨突)的变异,在解剖教科书中已有了记载;在欧美文献中许多学者已作过专题研究。虽然已有许多报导,但研究的多系外国人材料,而对中国人材料进行研究者还很少。经查仅有Wood-Jones就华北人及陆振山就华西人的颅骨进行     

花粉对抗体生成的影响

LACA雄性小鼠经腹腔注射花粉浸提液,其抗体生成受到一定的抑制,IgG、PFC抑制率为56.2～63.6％,血清抗体生成量的抑制车为42％,主要由花粉浸提液中MW>1000的组分昕致。LACA雄性小鼠经灌胃给予花粉浸提液,对lgG、PFC及血清抗体生成量均未见有明显影响。