ⅠL-33、ⅠL-35在晚期胃癌术后加味五苓散联合超声药物透入治疗中的临床意义

目的：分析加味五苓散联合超声药物透入在晚期胃癌患者术后恢复胃肠道功能中的价值。方法将68例行晚期胃癌术后患者随机分为试验组和对照组,2组综合治疗相同,试验组加用加味五苓散联合超声药物透入治疗,观察2组患者血清ⅠL-33、血清ⅠL-35、腹围变化、肠鸣音恢复、肛门排气排便及住院时间。结果试验组血清ⅠL-33、ⅠL-35较对照组明显下降（ P＜0.05）;2组腹围差在治疗后48 h、72 h、96 h及120 h时比较差异均有统计学意义（ P均＜0.05）;2组肠鸣音恢复、排气、排便时间比较差异均有统计学意义（ P均＜0.05）。结论加味五苓散联合超声药物透入治疗可促进胃癌患者术后肠鸣音恢复、加快胃肠功能、减轻腹胀;血清ⅠL-33、ⅠL-35可评估治疗效果。     

氢溴酸山莨菪碱在脓毒症/脓毒性休克治疗中的研究进展

脓毒症是一个高病死率的疾病,炎症因子风暴及微循环障碍导致多器官功能衰竭是脓毒症/脓毒性休克的重要病理生理过程。氢溴酸山莨菪碱可以保护内毒素所致的脏器损伤、抑制炎症反应、有效改善微循环障碍,在临床上主要应用于脓毒性休克的治疗。本文拟从脓毒症/脓毒性休克的治疗新理念、氢溴酸山莨菪碱在脓毒症/脓毒性休克治疗的研究进展等方面进行综述,为早期阻断与预防脓毒症/脓毒性休克提供新的思路。     

细菌生物膜在肿瘤生物学进程及治疗应用中的研究进展

细菌生物膜是细菌及其胞外聚合物形成的微生物群体,具有增强抗生素抵抗及抗宿主免疫等能力。肿瘤微环境中的细菌生物膜具有诱导炎症-癌症转化、促进癌细胞增殖转移和增强肿瘤耐药性等作用,影响着肿瘤的生物学进程。此外,细菌生物膜还被证实可协同抗癌药物抑制肿瘤细胞增殖、促进抗癌药物靶向定植及增强宿主免疫功能。本文就细菌生物膜在影响恶性肿瘤发生发展及抗肿瘤治疗中的研究进行综述。     

miR-21 targets Fas ligand-mediated apoptosis in breast cancer cell line MCF-7

Over-expression of Fas ligand （FasL） on tumor cell surface can induce the apoptosis of spe- cific activated tumor infiltrating lymphocytes （TILs） via the Fas/FasL pathway, leading to the formation of a site of immune privilege surrounding the tumor mass for escaping immune surveillance and pro- moting tumor proliferation, invasion and metastasis. The blocking effect of miR-21 on FasL-mediated apoptosis in breast cancers was investigated in this study. The expression levels of miR-21 and FasL in human breast carcinoma cell lines were detected by using RT-PCR and Western blotting. FasL as a tar- get gene of miR-21 was identified by Luciferase assay. The apoptosis of Jurkat T lymphocytes induced by MCF-7 cells was determined by flow cytometry. It was found that in four human breast cancer cell lines, FasL expression level in MCF-7 cells was the highest, while miR-21 was down-regulated the most notably. After miR-21 expression in MCF-7 cells was up-regulated, FasL was identified as a target gene of miR-21. When the effector/target （E/T） ratio of MCF-7 cells and Jurkat cells was 10：1, 5：1 and 1：1, the inhibitory rate of apoptosis of Jurkat T lymphocytes induced by MCF-7 cells was 95.81%, 93.16% and 91.94%, respectively. It is suggested that in breast cancers miR-21 expression is negatively associ- ated with FasL expression, and FasL is a target gene of miR-21, miR-21 targeting and regulating FasL-mediated apoptosis will bring us the possibility of a new tumor immunotherapy via breaking tu- mor immune privilege.     

Quercetin suppresses HeLa cells by blocking PI3K/Akt pathway

To explore the effect of quercetin on the proliferation and apoptosis of HeLa cells, HeLa cells were incubated with quercetin at different concentrations. Cell viability was evaluated by MTT assay, cell apoptosis was detected by Annexin- V/PI double labeled cytometry and DNA ladder assay. Cell cycle was flow cytometrically determined and the morphological changes of the cells were ob- served under a fluorescence microscope after Hoechst 33258 staining and the apoptosis-related pro- teins in the HeLa cells were assessed by Western blotting. The results showed that quercetin signifi- cantly inhibited the growth of HeLa cells and induced obvious apoptosis in vitro in a time- and dose-dependent manner. Moreover, quercetin induced apoptosis of HeLa cells in cell cycle-dependent manner because quercetin could induce arrest of HeLa cells at G0/G1 phase. Quercetin treatment down-regulated the expression of the PI3K and p-Akt. In addition, quercetin could down-regulate ex- pression of bcl-2, up-regulate Bax, but exerted no effect on the overall expression of Akt. We are led to conclude that quercetin induces apoptosis via PI3k/Akt pathways, and quercetin has potential to be used as an anti-tumor agent against human cervix oancer.     

人文护理在肿瘤患者临终关怀中的应用进展

本文就临终关怀中护理的内涵、特点,临终关怀对护理的要求,以及人文护理模式在临终关怀这一特殊医疗领域中的具体应用进行综述,在于探讨人文方面的护理方式运用于肿瘤病人临终关怀的可行性和有效性。     

阴道发育异常247例临床分析

目的:探讨阴道发育异常的临床特征和诊治情况。方法:回顾性分析2002年至2011年在我院就诊的247例阴道发育异常患者的临床资料。结果:247例阴道发育异常患者中先天性无阴道173例(70.04%),阴道闭锁65例(26.32%),阴道隔膜9例(3.64%)。临床表现主要为无月经来潮(96.4%)、腹痛(32.4%)和性生活困难(8.9%)。其中合并子宫畸形患者201例(81.4%),行肾脏B超184例中合并肾脏畸形患者19例(10.3%)。247例阴道发育异常患者中235例行手术治疗。结论:阴道发育异常以先天性无阴道所占比例最大,可伴有子宫发育畸形和泌尿系统畸形,伴发畸形的诊断有重要临床意义。阴道发育异常患者大多需手术治疗,具体术式根据病变类型决定。     

产科急症:产前出血-子痫-HELLP综合征

孕晚期,产时及产后的急性出血都需紧急处理,并在短时间内作出鉴别诊断,还需麻醉科及新生儿科配合。本文介绍诊断及处理应遵循的原则。     

腹股沟神经卡压征的应用解剖

目的：为腹股沟神经卡压征的防治提供解剖基础。方法：在50侧(25具)成年男性尸体标本上对腹股沟区皮神经行解剖学观测。结果：髂腹下神经皮支6％(3侧)，髂腹股沟神经皮支90％(45侧)，生殖股神经生殖支的皮支42％(21侧)，髂腹股沟神经与生殖股神经生殖支形成的吻合支12％(6侧)，生殖股神经股支的皮支8％(4侧)。结论：腹股沟区皮神经行程变异很大，解剖变异是导致神经卡压的主要原因。