Fc⁃gammaⅠ型受体在阿尔茨海默病患者和模型小鼠脑皮质的表达

目的 探讨高亲和力IgG受体Fc-gammaⅠ型(FcγRⅠ)在阿尔茨海默病(AD)脑皮层的表达变化。方法 选用正常人脑组织样本和神经病理诊断为AD患者脑组织样本，6月龄野生型小鼠和APP/PS1小鼠。免疫荧光和免疫蛋白印迹检测脑皮层FcγRⅠ的细胞定位和表达。结果 人和小鼠脑皮层的神经元均普遍表达FcγRⅠ。与健康人脑相比，AD患者脑皮层的FcγRⅠ表达显著降低(P<0.05)。与野生型小鼠脑相比，AD模型小鼠脑皮层的FcγRⅠ表达也呈现出显著降低(P<0.05)。结论 IgG高亲和力受体FcγRⅠ在AD患者脑和AD模型小鼠脑皮层的表达显著下降，可能与AD的神经病理机制相关。     

携RHD-CE(2-9)-D基因并产生抗-D、抗-E抗体1例

RHD-CE(2-9)-D属融合基因[1,2],为RHD基因的一种弱表现形式,常规血清学试验常将其鉴定为RhD–。据文献报道,Rh血型系统D抗原抗原性强于E抗原[3-6],理论上机体接受D抗原和E抗原免疫性刺激后产生的抗体常规应该是抗-D强于抗-E。然而,我们临床工作中遇到了抗-E(1∶512)强于抗-D(1∶64),与常规不符,探究详情报道如下。     

四月福建党史上的今天

1966年4月4日至11日中共福建省委“小三线”建设领导小组在福州召开“小三线”建设工作会议。2015年4月8日国务院印发通知,批准《中国(福建)自由贸易试验区总体方案》。1932年4月11日至12日毛泽东在龙岩主持会议,部署攻打漳州计划。4月20日,毛泽东率领工军东路军攻克漳州,巩固发展了闽西苏区,为第四次反“围剿”斗争做了物资准备。     

Shenling Baizhu Powder (参苓白术散) Ameliorates Pi (Spleen)-Deficiency-Induced Functional Diarrhea in Rats

Objective To explore the mechanism of Pi(Spleen)-deficiency-induced functional diarrhea(FD)model rats treated by Shenling Baizhu Powder(参苓白术散,SBP).Methods Thirty male Sprague-Dawley rats were randomly divided into 5 groups including control,model,low-,medium-,and high-dose SBP groups(SBPLDG,SBPMDG,SBPHDG),6 rats in each group,respectively.Pi-deficiency-induced FD rats model was developed through Radix et Rhizoma Rhei gavage for 7 days.After modeling,the rats were treated with 3 doses of SBP[0.93,1.86,and 3.72 g/(kg·d)],and the rats in the control and model groups were given pure water for 7 days.The diarrhea index was calculated.On the 7th and 14th days,the traveled distance of rat was measured by the open field test.Serum D-xylose content was determined by the phloroglucinol method and interleukin(IL)-10 and IL-17 levels were measured using an enzyme-linked immunosorbent assay kit.The content of Treg cells was determined by flow cytometry.Results Compared with the control group,the diarrhea index and IL-17 level in the model group were significantly higher and the total exercise distance and D-xylose content significantly decreased(P>0.05).The expression of IL-10 in the SBPHDG group was significantly up-regulated,and serum D-xylose level and Treg cells increased significantly compared with the model group(P>0.05).Conclusion High-dose SBP exhibited ameliorating effects against Pi-deficiency induced FD,which might be attributed to its modulations on intestinal absorption function as well as adaptive immunity in mesenteric lymph nodes of rat.     

Patient-derived non-small cell lung cancer xenograft mirrors complex tumor heterogeneity

Objective:Patient-derived xenograft(PDX)models have shown great promise in preclinical and translational applications,but their consistency with primary tumors in phenotypic,genetic,and pharmacodynamic heterogeneity has not been well-studied.This study aimed to establish a PDX repository for non-small cell lung cancer(NSCLC)and to further elucidate whether it could preserve the heterogeneity within and between tumors in patients.Methods:A total of 75 surgically resected NSCLC specimens were implanted into immunodeficient NOD/SCID mice.Based on the successful establishment of the NSCLC PDX model,we compared the expressions of vimentin,Ki67,EGFR,and PD-L1 proteins between cancer tissues and PDX models using hematoxylin and eosin staining and immunohistochemical staining.In addition,we detected whole gene expression profiling between primary tumors and PDX generations.We also performed whole exome sequencing(WES)analysis in 17 first generation xenografts to further assess whether PDXs retained the patient heterogeneities.Finally,paclitaxel,cisplatin,doxorubicin,atezolizumab,afatininb,and AZD4547 were used to evaluate the responses of PDX models to the standard-of-care agents.Results:A large collection of serially transplantable PDX models for NSCLC were successfully developed.The histology and pathological immunohistochemistry of PDX xenografts were consistent with the patients’tumor samples.WES and RNA-seq further confirmed that PDX accurately replicated the molecular heterogeneities of primary tumors.Similar to clinical patients,PDX models responded differentially to the standard-of-care treatment,including chemo-,targeted-and immuno-therapeutics.Conclusions:Our established PDX models of NSCLC faithfully reproduced the molecular,histopathological,and therapeutic characteristics,as well as the corresponding tumor heterogeneities,which provides a clinically relevant platform for drug screening,biomarker discovery,and translational research.     

~(60)Co-γ射线辐照对黄精中微生物及多糖的影响

目的研究~(60)Co-γ射线辐照对黄精中微生物数量及多糖含有量的影响。方法参照2015年版《中国药典》方法,检测需氧菌总数(TAMC)、霉菌和酵母菌总数(TYMC)、耐热菌数、大肠埃希菌;采用硫酸蒽酮比色法,检测波长582 nm,于辐照后0、1、3、6月进行相应检测。结果黄精经0.5～6.0 kGy ~(60)Co-γ射线辐照后,微生物数量显著减少,并随着辐照剂量增加而更明显;多糖含有量显著降低,也随着辐照剂量增加而更明显。结论 3.0～6.0 kGy ~(60)Co-γ射线辐照能有效杀灭黄精中微生物,但也可能使多糖含有量降低。     

股内侧肌悬吊髌骨单锚钉双束解剖重建内侧髌股韧带

[目的]介绍股内侧肌悬吊髌骨单锚钉双束解剖重建内侧髌股韧带(MPFL)治疗复发性髌骨脱位的技术方法。[方法]2013年1月～2016年6月收治的复发性髌骨脱位患者19例。手术以股骨内上髁、收肌结节与腓肠肌结节之间的凹陷作为MPFL股骨止点,建立直径6 mm、深40 mm骨隧道;髌骨内缘中点做髌骨侧止点,沿髌骨长轴建立约1 cm的骨槽,在中点平行于髌骨关节面打入1枚带双线4.5 mm锚钉,将移植物固定于骨槽并与周围软组织缝合。移植物近侧游离端穿过股内侧肌斜束肌腱,形成"悬吊"结构并自股骨侧切口下穿出,远侧游离端在深筋膜隧道自近端移植物同一位置穿出,远近端缝合为一股端,于骨道内口用可吸收界面螺钉固定。[结果]术后随访12～48个月。未发生感染、膝关节功能障碍、复发等相关并发症。末次随访时膝关节功能Lysholm、IKDC、Kujala和Tegner评分均较术前显著增加,差异有统计学意义(P0.05);末次随访时CA、Q角、PTA、PLSR均较术前显著减小,差异有统计学意义(P0.05)。[结论]股内侧肌悬吊髌骨拴桩双束解剖重建内侧髌股韧带切口小、复发率低,可恢复髌股轨迹的动力和静力稳定性,临床疗效满意。     

介入联合胫骨横向搬移治疗下肢缺血性疾病

[目的]比较介入治疗联合胫骨横向搬移与单纯介入手术治疗下肢缺血性疾病,包括血栓闭塞性脉管炎(TAO)、动脉硬化闭塞症(TSO)的临床效果。[方法]回顾性分析本院2015年4月～2017年4月收治的42例42肢下肢缺血性疾病患者,其中,介入-骨搬移组22例,应用介入治疗解决近端血管阻塞问题后采用胫骨横向搬移治疗;单纯介入组20例采用单纯介入治疗。采用视觉模拟疼痛评分(VAS)和生活质量综合评估(SF36)评价临床效果,测量第1足趾皮温、并行下肢血管CTA检查。[结果]两组患者均顺利手术,术中无严重并发症。两组术后随访时间12～23个月。虽然入院时、术后1个月两组间VAS评分、第1足趾皮温和SF36评分的差异无统计学意义(P0.05),但术后3、12个月时,介入-骨搬移组VAS评分显著低于单纯介入组,介入-骨搬移组第1足趾皮温显著高于单纯介入组,介入-骨搬移组SF36评分和生理机能(PF)、生理职能(RP)、躯体疼痛(BP)、健康状况(GH)亚评分显著优于单纯介入组,差异均有统计学意义(P0.05)。下肢动脉CTA显示介入-骨搬移组于术后3个月可见明显侧枝血管网形成,显著优于单纯介入组。[结论]介入治疗联合胫骨横向搬移可以重建下肢缺血性疾病患者远端肢体侧枝循环,远期疗效优于单纯介入治疗,可以更好地改善患者症状,提高生活质量。