The Cotard syndrome. Report of two patients: with a review of the extended spectrum of 'délire des négations'

The Cotard syndrome is characterized by the delusion where an individual insists that he has died or part of his body has decayed. Although described classically in schizophrenia and bipolar disorder, physical disorders including migraine, tumour and trauma have also been associated with the syndrome. Two new cases are described here, the one associated with arteriovenous malformations and the other with probable multiple sclerosis. The delusion has been embarrassing to each patient. Study of such cases may have wider implications for the understanding of the psychotic interpretation of body image, for example that occurring in anorexia nervosa.     

重楼甾体总皂苷对血小板聚集的直接诱导作用及初步机制研究

目的:确定重楼甾体总皂苷(TSSP)体外直接诱导血小板聚集作用并对其可能的机制进行探索.方法:比浊法测定血小板聚集,透射电镜观察血小板形态学改变.结果:体内外血小板聚集模型研究表明,TSSP体内给药能够增强ADP诱导血小板聚集.体外能够直接诱导血小板聚集,并呈剂量效应关系.电镜观察TSSP能够直接激活血小板引起变形释放等反应.肾上腺素能够增强TSSP诱导的血小板聚集,该增强作用能被酚妥拉明所拮抗.蛋白酪氨酸磷酸酶抑制剂过钒酸钠能够增强TSSP诱导血小板聚集.结论:本研究表明,TSSP中含有参与止血作用的药理活性成分.     

AM真菌对滇重楼光合参数及生理指标的影响

通过室温盆栽接种试验与室内分析相结合的方法,观察灭菌土壤条件下接种28种丛枝菌根(AM)真菌对滇重楼光合参数和生理生化指标的影响。结果表明,滇重楼与AM真菌形成了良好的共生关系。AM真菌诱导处理后,不同AM真菌对滇重楼叶片的光合参数和生理生化指标的影响有所差异,AM真菌的施用能够提高滇重楼叶肉细胞的光合作用强度,提高滇重楼叶片可溶性蛋白和可溶性糖含量及保护酶活性,增强了滇重楼抗不利环境的能力,促进了滇重楼的生长,而滇重楼与AM真菌之间存在一定的相互选择性。从综合接种效应来看,瑚状盾巨囊霉Racocetra coralloidea、美丽盾巨孢囊霉Scutellospora calospora、近明球囊霉Claroideoglomus claroideum、透明盾巨孢囊霉S. pellucida和明球囊霉Rhizophagus clarus是最适宜滇重楼大田栽培时推广应用的优良备选菌株。     

Immunofluorescence serology. A tool for prognosis of Q-fever

The indirect immunofluorescence antibody test is currently the method of choice for Q-fever laboratory diagnosis. It permits the detection of IgG-, IgM-, and IgA-specific antibodies against the two phases of Coxiella burnetii. Sera from 20 cases of C. burnetii infection have been examined. Only total IgG against phase II were detected in cryptic infections. In acute Q-fever cases, the appearance of total IgG antibodies against phase I was a sign of aggravation, while IgM titers remained low. In subacute cases of Q-fever, anti-phase-I IgG titers were equal to or higher than anti-phase-II titers, and IgM against both phases were produced over a long time. Particularly high IgM titers were found in cases of granulomatous hepatitis. IgA antibodies against phase I were found in cases of Q-fever endocarditis, although the two cases that died had few or no IgA antibodies, despite very high IgG and IgM titers.     

Incidence of varying factors on the immunochemical behavior of alpha 1-acid glycoprotein

Electroimmunodiffusion methods of Laurell and radial immunodiffusion method of Mancini are compared for the qualitative and quantitative analysis of native and desialylated alpha 1-acid glycoprotein. Samples are incubated under different conditions at decreasing pH (3.5 to 0.5 pH units), with increasing ionic strength and with neuraminidase during different time intervals. Results show a pronounced decrease in electrophoretic mobility of alpha 1-acid glycoprotein treated either with acidic reagents or with neuraminidase (ionic strength has no effect). Such a procedure might involve chemical or enzymatic hydrolysis by which sialyl residues are removed. This hydrolysis implicates lower results in the estimation of the desialylated glycoprotein by electroimmunodiffusion. On the other hand, the amounts of alpha 1-acid glycoprotein evaluated by radial immunodiffusion are not modified after incubation. This is expected since diffusion and antigenic properties are not related to the sialic acid content. The data suggest that radial immunodiffusion, less accurate and sensitive than electroimmunodiffusion, is nevertheless more adequate for estimating native and desialylated alpha 1-acid glycoprotein.