江苏社区人群亚临床甲状腺功能异常和血压关系的研究

通过对6044名江苏社区人群横断面流行病学调查发现,亚临床甲状腺功能异常(亚临床甲状腺功能亢进症/亚临床甲状腺功能减退症)与血压的升高无明显相关性.     

江苏社区人群亚临床甲状腺功能异常和血压关系的研究

通过对6044名江苏社区人群横断面流行病学调查发现,亚临床甲状腺功能异常(亚临床甲状腺功能亢进症/亚临床甲状腺功能减退症)与血压的升高无明显相关性.     

肾综合征出血热的诊断和治疗

肾综合征出血热 (HFRS)是我省常见病 ,其病情凶险 ,预后较差。因此 ,对本病进行早期诊断及合理治疗极为关键。现对 HFRS的诊断和治疗概述如下。1　 HFRS诊断1.1　流行病学资料　包括流行季节发病、生活在疫区或 3天至 2个月内到过疫区 ;与鼠类等宿主动物及其排泄物接触史、鼠咬伤史或食用鼠类污染食物史。1.2　临床特征　典型病例有发热、出血和肾损害三大主征 ,具有发热期、低血压休克期、少尿期、多尿期和恢复期五期经过。不典型者可有越期或前三期重叠。热退后症状加重有助于诊断 [1 ] 。1.3　实验室检查　血常规检查示早期白细胞计…     

大鼠肝细胞生长因子与肝再生增强因子融合基因真核表达质粒的构建及鉴定

Objective To construct and identify an eukaryotic expression plasmid containing rat hepatocyte growth factor(rHGF)gene and rat augmenter of liver regeneration(rALR)gene,so that to provide experimental basis for developing new treatments of hepatic fibrosis.Methods The gene fragments of rHGF and rALR were amplified from recombinant prokaryotic plasmid pUC18-rHGF and pUC18-rALR by polymerase chain reaction(PCR),respectively,then were spliced by overlap extension PCR with a linker,and the fusion gene rHGF-linker-rALR was constructed.The fusion gene was directionally inserted into the eukaryotic expression plasmid pcDNA3.1 between restriction sites of Kpn Ⅰ and Xba Ⅰ to construct the recombinant eukaryotic expression plasmid pcDNA3.1-rHGF-linker-rALR,and the new constructed recombinant plasmid was identified by double restriction digestion and DNA sequencing.Results DNA fragments of 2200 bp and 400 bp were observed after the electrophoresis of products amplified from recombinant prokaryotic plasmid pUC18-rHGF and pUC18-rALR,respectively,which was consistent with the theoretical value.The electrophoresis of fusion gene rHGF-linker-rALR obtained by overlap extension PCR technique showed only a 2 600 bp DNA fragment,which was in accordance with the expected value.Electrophoresis of products of pcDNA3.1-rHGF-linker-rALR digested with Kpn Ⅰ and Xba Ⅰ showed two DNA fragments with 2600 bp and 5400 bp,which were both consistent with the expected value.The sequences were confirmed correctly by DNA sequencing.Conclusion The recombinant eukaryotic expression plasmid pcDNA3.1-rHGF-linker-rALR is successfully constructed,which provides experimental basis for developing gene therapy of hepatic fibrosis.     

老年人发热病因及临床特征分析

目的分析老年人发热性疾病的病因与临床特征。方法收集温州医学院附属第一医院2001-01~2004-04以发热为首发临床表现住院、资料完整且符合发热诊断标准的患者87例,进行回顾性分析。结果87例患者中,感染性疾病44例,结缔组织病4例,恶性肿瘤9例,其他疾病5例,诊断不明25例。感染性疾病中结核病占6例(13·6%)。B超、X线胸片、CT、MRI、骨髓穿刺、组织活检检查的确诊率分别为21·1%、16·9%、63·0%、0、16·7%、57·1%。结论老年人发热性疾病由于症状不典型,诊断较困难。感染性疾病仍然是老年人主要的发热原因。详细的有针对性的病史询问、查体及常规实验室检查(特别是血沉)对发热性疾病的诊断很重要,早期、合理使用影像学检查技术及必要的侵入性检查对疾病诊断有一定帮助。     

椎间孔镜治疗腰椎间盘突出症的近期疗效观察

<正>经皮椎间孔镜技术(PTED)是一种新型的微创治疗方法,可治疗经保守治疗无效的腰椎间盘突出症。我院已引进了该项技术,并在6个月内开展了35例手术,全部得到了回访,近期疗效满意,现总结报告如下。1资料与方法 1.1临床资料:大同市第五人民医院于2013年1至7月应用PTED治疗腰椎间盘突出症患者共35例,其中男性19例,女性16例,年龄25⁶3岁,平均(36±14)岁。病程为1663岁,平均(36±14)岁。病程为16²3个月,平均(21±4)     

“刀光剑影”中走出来的16岁发明家徐紫宸

16岁的她,看上去是一个普通的高中小女生,戴着一副眼镜,斯斯文文的样子.站在第八届中国青少年科技创新奖颁奖台上,她激动地说：“创新来自兴趣,创新源于梦想,那是支撑我的科学创新之路的不竭动力.”徐紫宸,RoboCup青少年机器人世界杯铜奖、重庆青少年科技创新市长奖等奖项获得者.