全文获取类型
收费全文 | 4365篇 |
免费 | 1074篇 |
国内免费 | 427篇 |
专业分类
耳鼻咽喉 | 15篇 |
儿科学 | 31篇 |
妇产科学 | 68篇 |
基础医学 | 874篇 |
口腔科学 | 99篇 |
临床医学 | 464篇 |
内科学 | 479篇 |
皮肤病学 | 42篇 |
神经病学 | 148篇 |
特种医学 | 77篇 |
外国民族医学 | 1篇 |
外科学 | 259篇 |
综合类 | 867篇 |
预防医学 | 94篇 |
眼科学 | 100篇 |
药学 | 408篇 |
中国医学 | 72篇 |
肿瘤学 | 1768篇 |
出版年
2024年 | 27篇 |
2023年 | 287篇 |
2022年 | 480篇 |
2021年 | 770篇 |
2020年 | 697篇 |
2019年 | 480篇 |
2018年 | 330篇 |
2017年 | 345篇 |
2016年 | 364篇 |
2015年 | 600篇 |
2014年 | 474篇 |
2013年 | 300篇 |
2012年 | 205篇 |
2011年 | 194篇 |
2010年 | 105篇 |
2009年 | 60篇 |
2008年 | 26篇 |
2007年 | 8篇 |
2006年 | 1篇 |
2005年 | 4篇 |
2004年 | 1篇 |
2003年 | 2篇 |
2001年 | 1篇 |
1998年 | 1篇 |
1995年 | 1篇 |
1994年 | 2篇 |
1992年 | 1篇 |
1989年 | 3篇 |
1988年 | 1篇 |
1985年 | 9篇 |
1984年 | 21篇 |
1983年 | 15篇 |
1982年 | 13篇 |
1981年 | 11篇 |
1980年 | 5篇 |
1979年 | 3篇 |
1978年 | 8篇 |
1977年 | 1篇 |
1976年 | 2篇 |
1975年 | 6篇 |
1974年 | 1篇 |
1973年 | 1篇 |
排序方式: 共有5866条查询结果,搜索用时 93 毫秒
41.
Colorectal cancer (CRC) is one of the most common malignancy cancers in the world. Aberrant microRNA expression is involved in human diseases including cancer. In the present study, we investigated the miR-892a's role in CRC cell proliferation. We found that miR-892a was frequently upregulated in human colorectal cancer tissues and cell lines compared with the matched tumor adjacent tissues and normal colonic cell line FHC. Overexpression of miR-892a promoted cell proliferation and colony formation of CRC. Bioinformatics analysis further revealed PPP2R2A was identified as a potential miR-892a. Overexpression of miR-892a-in SW480 cells reduced PPP2R2A protein expression. Subsequently, data from luciferase reporter assays showed that PPP2R2A 3′-untranslated region (3′-UTR) carried the directly binding site of miR-892a. Furthermore, siRNA-mediated silencing of PPP2R2A blocked the inhibitory effect of miR-892a-in on CRC cell growth. In sum, our data provided compelling evidence that overexpression of miR-892a may provide a selective growth promotion for CRC cells by direct suppression of PPP2R2A expression. 相似文献
42.
《Molecular immunology》2015,66(2):293-301
Much evidence demonstrates that microglia mediated inflammatory responses play an important role in brain injury in ischemia. miRNA is the important factor in regulation of inflammation. However, the effect of miRNA in microglia mediated inflammatory responses has not been well studied. In the study, we demonstrate that miR-203 negatively regulates ischemia induced microglia activation by targeting MyD88, an important adapter protein involved in most Toll-like receptors (TLRs) and interleukin-1 receptor (IL-1R) pathways. Through negative feedback, enforced expression of miR-203 or MyD88 siRNA silencing inhibits downstream NF-κβ signaling and microglia activation, thereby alleviating neuronal injury. These findings reveal that miR-203 represents a novel target regulating neuroinflammation and brain injury, thus offering a new therapeutical strategy for cerebral hypoxic diseases. 相似文献
43.
44.
Yunpeng Sun Wenjun Yang Yunfeng Shan Qiyu Zhang Huanhuan Wu 《Cancer biology & therapy》2019,20(6):729-739
Pancreatic ductal adenocarcinoma (PDAC) remains a leading cause of cancer-related death due to the failure of traditional therapies. In the present study, we attempted to construct a lncRNA-miRNA-mRNA network which may modulate PDAC cell proliferation and Gemcitabine-induced cell apoptosis starting from CDK14, a new member of the CDK family and an oncogene in many cancers. Based on TCGA data, a significant positive correlation was observed between lncRNA MSC-AS1 and CDK14. Moreover, MSC-AS1 expression was upregulated in PDAC tissues. Higher MSC-AS1 expression was correlated with poorer prognosis in patients with PDAC. MSC-AS1 knockdown in Panc-1 and BxPC-3 cells significantly inhibited the cell proliferation. Moreover, miR-29b-3p, which has been reported to act as a tumor suppressor, was predicted to bind to both MSC-AS1 and CDK14. Contrary to MSC-AS1, higher miR-29b-3p expression was correlated to better prognosis in patients with PDAC. In both PDAC cell lines, miR-29b-3p negatively regulated MSC-AS1 and CDK14. As confirmed using luciferase reporter gene and RIP assays, MSC-AS1 served as a ceRNA for miR-29b-3p to counteract miR-29b-mediated CDK14 repression. MSC-AS1 knockdown inhibited CDK14 protein levels and PDAC proliferation and enhanced gemcitabine-induced cell death and apoptosis while miR-29b-3p inhibition exerted an opposing effect; the effect of MSC-AS1 knockdown was partially attenuated by miR-29b-3p inhibition. Taken together, we demonstrated that MSC-AS1/miR-29b-3p axis modulates the cell proliferation and GEM-induced cell apoptosis in PDAC cell lines through CDK14. We provided a novel experimental basis for PDAC treatment from the perspective of lncRNA-miRNA-mRNA network. 相似文献
45.
Li Jiang Chang-Liang Wu Ji-Zhou Wu Xia Yang Han-lin Wang Guo-Jian Li 《Pathology, research and practice》2019,215(1):68-81
The clinical role and potential molecular mechanisms of microRNA-449c-5p (miR-449c-5p) in hepatocellular carcinoma (HCC) tissues remains unclear. Combining multiple bioinformatic tools, we studied the miR-449c-5p expression levels in HCC tissues and explored possible target genes and related signaling pathways. First, miR-449c-5p expression data from microarrays provided by publicly available sources were mined and analyzed using various meta-analysis methods. Next, genes that were downregulated after miR-449c-5p mimic transfection into HCC cells were identified, and in silico methods were used to predict potential target genes. Several bioinformatic assessments were also performed to evaluate the possible signaling pathways of miR-449c-5p in HCC. Five microarrays were included in the current study, including GSE98269, GSE64632, GSE74618, GSE40744 and GSE57555. The standard mean difference was 0.44 (0.07–0.80), and the area under the curve was 0.68 (0.63–0.72), as assessed by meta-analyses, which consistently indicated the upregulation of miR-449c-5p in HCC tissues. A total of 2244 genes were downregulated after miR-449c-5p mimic transfection into an HCC cell line, while 5217 target genes were predicted by in silico methods. The overlap of these two gene pools led to a final group of 428 potential target genes of miR-449c-5p. These 428 potential target genes were primarily enriched in the homologous recombination pathway, which includes DNA Polymerase Delta 3 (POLD3). Data mining with Oncomine and the Human Protein Atlas showed a decreasing trend in POLD3 mRNA and protein levels in HCC tissue samples. This evidence suggests that miR-449c-5p could play an essential role in HCC through various pathways and that POLD3 could be a potential miR-449c-5p target. However, these in silico findings should be validated with further experiments. 相似文献
46.
Xiaolan Huang Liping She Xiangmin Luo Shuzhen Huang Jinxiang Wu 《Pathology, research and practice》2019,215(5):918-923
Polycystic ovary syndrome (PCOS) is one of the most complex and common reproductive and endocrinologic disorders in the child-bearing age of women. Recently, miR-222 were reported to be associated with the etiology of PCOS. However, the function of miR-222 during the pathogenesis of PCOS remains unclear. In the present study, we aimed to investigate the role of miR-222 in PCOS. Firstly, miR-222 expression was examined by quantitative real-time PCR (qRT-PCR) in PCOS. The effects of miR-222 on proliferation, apoptosis and cell cycle in KGN cells were analyzed by CCK-8 assay and flow cytometry analysis, respectively. In addition, bioinformatics analysis was used to predict the target genes of miR-222, and dual-luciferase reporter assay was applied to verified the interaction between miR-222 and p27 Kip1 in KGN cells. Moreover, the expressions of p27 Kip1 in KGN cells treated with miR-222 mimics or miR-222 inhibitor were evaluated by qRT-PCR and western blot assays. The results showed that the expression of miR-222 was remarkably upregulated in PCOS tissues compared with corresponding normal tissues. In the gain-of-function and loss-of-function assays, we revealed that miR-222 mimics significantly promoted cell proliferation, while miR-222 inhibitor induced cell apoptosis and cell cycle arrested. Furthermore, p27 Kip1 was identified as a target gene of miR-222, and could be negatively regulated by miR-222 mimics in KGN cells. In conclusion, our findings suggested that miR-222 may promote the progression of PCOS by targeting p27 Kip1. 相似文献
47.
48.
49.
《中国现代医生》2020,58(2):34-37+41
目的研究中缝核miR-16以及5-羟色胺转运体(serotonin transporter,SERT)的表达在失眠症发病机制中的作用。方法 20只雌性SD大鼠,随机分成对照组和失眠组。失眠组大鼠接受腹腔注射对氯苯丙氨酸(pchlorophenalanine,PCPA)350 mg/(kg·d),连续3 d,对照组给予等量生理盐水。取中缝核组织,测定其miR-16、SERT m RNA与SERT蛋白水平,进行注射前后的组间比较。结果失眠组大鼠中缝核miR-16相对水平(1.23±0.33)与对照组(0.71±0.25)比较明显升高,差异具有统计学意义(t=6.68,P=0.000);而失眠组SERT mRNA相对水平(0.68±0.14)以及SERT蛋白相对水平(0.28±0.11)显著低于相应的对照组(1.06±0.30,0.57±0.15)(t=3.94,P=0.004;t=4.69,P=0.002)。结论中缝核miR-16以及SERT的异常表达,可能参与了失眠症的发病机制。 相似文献
50.
目的:研究miR-194、EZH2对甲状腺癌细胞增殖、迁移和侵袭的影响,并探讨其作用机制。方法:采用qRT-PCR法检测甲状腺癌组织、癌旁正常组织及甲状腺癌细胞和正常甲状腺上皮细胞中miR-194和EZH2的mRNA表达;将miR-194(转染miR-194 mimics)、miR-NC(未转染细胞)、inhibitor-NC(转染空inhibitor)、miR-194 inhibitor(转染miR-194 inhibitor)、si-EZH2(转染si-EZH2)、miR-194+Vector(miR-194 mimics和pcDNA 3.1共转染)、miR-194+EZH2(miR-194 mimics和pcDNA 3.1-EZH2共转染)均以脂质体法转染到SW579、IHH-4细胞;Western blot检测细胞中EZH2的蛋白表达;MTT法检测细胞的增殖;Transwell检测细胞的迁移和侵袭;双荧光素酶报告基因检测实验检测细胞荧光素酶活性。结果:与正常组相比,甲状腺癌组EZH2的表达显著升高,miR-194的表达显著降低;与人正常甲状腺上皮细胞相比,甲状腺癌细胞中EZH2表达显著升高,miR-194的表达显著降低,且过表达miR-194和沉默EZH2均可抑制甲状腺癌细胞增殖、迁移和侵袭。EZH2为miR-194的靶标,且EZH2可逆转过表达miR-194对甲状腺癌细胞增殖、迁移和侵袭的抑制作用。结论:miR-194可抑制甲状腺癌细胞的增殖、迁移和侵袭,可能与靶向EZH2有关,将可为miR-194靶向治疗甲状腺癌提供依据。 相似文献